The distributions of HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 allele and haplotype at high-resolution level in Zhejiang Han population of China

2018 ◽  
Vol 46 (1) ◽  
pp. 7-16 ◽  
Author(s):  
Nanying Chen ◽  
Wei Wang ◽  
Fang Wang ◽  
Lina Dong ◽  
Shuoxian Zhao ◽  
...  
Keyword(s):  
High Resolution ◽  
Han Population ◽  
Resolution Level ◽  
Dqb1 Allele

Fiber optic multipoint high-resolution level sensor for biomedical applications

1995 ◽  
Author(s):  
Sergei N. Khotiaintsev ◽  
Victor de Leon Paredes ◽  
Esteban Molina-Flores ◽  
A. Zemliak ◽  
V. Svirid ◽  
...  
Keyword(s):  
High Resolution ◽  
Biomedical Applications ◽  
Fiber Optic ◽  
Level Sensor ◽  
Resolution Level

High-resolution donor-recipient HLA matching contributes to the success of unrelated donor marrow transplantation

Blood ◽  
2007 ◽  
Vol 110 (13) ◽  
pp. 4576-4583 ◽  
Author(s):  
Stephanie J. Lee ◽  
John Klein ◽  
Michael Haagenson ◽  
Lee Ann Baxter-Lowe ◽  
Dennis L. Confer ◽  
...  
Keyword(s):  
High Resolution ◽  
Human Leukocyte ◽  
The United States ◽  
Disease Stage ◽  
Unrelated Donor ◽  
Hla Matching ◽  
Leukocyte Antigen ◽  
Donor Factors ◽  
Resolution Level ◽  
Program Data

The relative importance of various human leukocyte antigen (HLA) loci and the resolution level at which they are matched has not been fully defined for unrelated donor transplantation. To address this question, National Marrow Donor Program data from 3857 transplantations performed from 1988 to 2003 in the United States were analyzed. Patient-donor pairs were fully typed for HLA-A, -B, -C, -DRB1, -DQB1, -DQA1, -DPB1, and -DPA1 alleles. High-resolution DNA matching for HLA-A, -B, -C, and -DRB1 (8/8 match) was the minimum level of matching associated with the highest survival. A single mismatch detected by low- or high-resolution DNA testing at HLA-A, -B, -C or -DRB1 (7/8 match) was associated with higher mortality (relative risk, 1.25; 95% CI, 1.13-1.38; P < .001) and 1-year survival of 43% compared with 52% for 8/8 matched pairs. Single mismatches at HLA-B or HLA-C appear better tolerated than mismatches at HLA-A or HLA-DRB1. Mismatching at 2 or more loci compounded the risk. Mismatching at HLA-DP or -DQ loci and donor factors other than HLA type were not associated with survival. In multivariate modeling, patient age, race, disease stage, and cytomegalovirus status were as predictive of survival as donor HLA matching. High-resolution DNA matching for HLA-A, -B, -C, and -DRB1 alleles is associated with higher rates of survival.

117-P: Comparison of High-Resolution HLA-A, B, Cw, DRB1, DQB1 Allele Distribution of Chinese Han With Other Five Ethic Groups

2010 ◽  
Vol 71 ◽  
pp. S92
Author(s):  
Yang Li ◽  
Jun He ◽  
Xiao Jing Bao ◽  
Qiao Cheng Qiu ◽  
Xiao Ni Yuan
Keyword(s):  
High Resolution ◽  
Chinese Han ◽  
Allele Distribution ◽  
Dqb1 Allele

scholarly journals The role of HLA-typing in transplantation of hematopoietic stem cells

2017 ◽  
Vol 5 (2) ◽  
pp. 159-153
Author(s):  
V. Khomenko
Keyword(s):  
High Resolution ◽  
Molecular Genetic ◽  
Human Leukocyte ◽  
Cost Effective ◽  
Hla Typing ◽  
Potential Donor ◽  
Hematopoietic Stem ◽  
Literary Sources ◽  
Resolution Level ◽  
Typing Methods

The system of human leukocyte antigen (HLA) and HLA-typing were used to match a potential donor with a recipient for allogeneic hematopoietic stem cell transplantation (HSCT). The HLA matching between donor and recipient is key role in allogeneic HSCT. The mismatch of HLA can cause graft rejection, graft-versus-host disease and decrease survival in patients receiving grafts from both related and unrelated donors. The adverse HLA effect on the outcome depends on the total number of mismatched alleles/loci and the resolution level of the mismatch (antigen or allele level).Thus, the final choice of compatible donor-recipient pairs should be based on high resolution molecular-genetic methods of HLA-typing. Serologic and molecular genetic methods of low resolution HLA-typing, which are cheaper than HLA-typing high-resolution, should be used for donor screening studies. HSCT from a fully compatible donor, matched high-resolution HLA-typing methods gives better results than from partially compatible. In some clinical circumstances, a partially compatible donor may be as effective as fully compatible. The selection of such a donor, taking into account the controversy of data from various literary sources, should be based on own research and experience. Creation and development of a Ukrainian database of donors with the HLA-haplotype specific to the indigenous population will make search of matching pairs of donor recipients more effective and cost-effective.

HLA-A, -B, -C, -DRB1 and -DQB1 allele and haplotype frequencies of 1463 umbilical cord blood units typed in high resolution from Bogotá, Colombia

2019 ◽  
Vol 80 (7) ◽  
pp. 425-426 ◽  
Author(s):  
Iván Aurelio Páez-Gutiérrez ◽  
David Guillermo Hernández-Mejía ◽  
Diana Vanegas ◽  
Bernardo Camacho-Rodríguez ◽  
Ana María Perdomo-Arciniegas
Keyword(s):  
High Resolution ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Haplotype Frequencies ◽  
Dqb1 Allele

HLA-DRB1, -DRB3, -DRB4 and -DRB5 genotyping at a super-high resolution level by long range PCR and high-throughput sequencing

2013 ◽  
Vol 83 (1) ◽  
pp. 10-16 ◽  
Author(s):  
Y. Ozaki ◽  
S. Suzuki ◽  
A. Shigenari ◽  
Y. Okudaira ◽  
E. Kikkawa ◽  
...  
Keyword(s):  
High Resolution ◽  
Long Range ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Resolution Level ◽  
Long Range Pcr

High Resolution Shadowing of DNA

1972 ◽  
Vol 30 ◽  
pp. 310-311
Author(s):  
R. Abermann ◽  
M. M. Salpeter
Keyword(s):  
Electron Beam ◽  
High Resolution ◽  
Cytochrome C ◽  
Test Specimen ◽  
Electron Beam Evaporation ◽  
Absolute Ethanol ◽  
Dna Molecules ◽  
Step Process ◽  
Carbon Coated ◽  
Resolution Level

High resolution shadowing by electron beam evaporation was used to visualize DNA molecules. Its applicability to the study of macromolecules at this resolution level was previously demonstrated on monomeric and dimeric tRNA particles.To utilize the potential of the high resolution shadowing for DNA, a mounting procedure was employed which provided a smooth substrate to which the naked molecules could be bound. This consisted of a two step process, modified from Kleinschmidt Lang, and Harford and Beer. When a 50μl droplet of cytochrome c (20 μg/ml in 0.2M Na acetate at pH 5) was allowed to stand on teflon for 20 minutes, a monolayer of protein formed on its surface. The film was picked up onto carbon coated grids, washed in ethanol and air dried. As test specimen, we used DNA from SV4O. The protein coated grids were floated for 30 minutes on buffer droplets containing 0.1-0.5 μg DNA/ml 10-3SSC and the molecules were bound to the protein monolayers by diffusion. Grids were then washed successively in 50% and absolute ethanol and air dried.

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