Identification, characterization and inhibition of Geotrichum pseudocandidum spoilage microbe in Gastrodia elata tuber

2021 ◽  
Author(s):  
Haiyan Sun ◽  
Xiao Liu ◽  
Jinjin Pei ◽  
Danqing Hao ◽  
Xinsheng Li ◽  
...  
Keyword(s):  
Gastrodia Elata

scholarly journals Structural Characterization of a Polysaccharide from Gastrodia elata and Its Bioactivity on Gut Microbiota

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4443
Author(s):  
Jiangyan Huo ◽  
Min Lei ◽  
Feifei Li ◽  
Jinjun Hou ◽  
Zijia Zhang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Citric Acid ◽  
Gut Microbiota ◽  
Structural Characterization ◽  
Hot Water ◽  
Nmr Analysis ◽  
Gastrodia Elata ◽  
Hot Water Extraction ◽  
Hydroxybenzyl Alcohol

A novel homogeneous polysaccharide named GEP-1 was isolated and purified from Gastrodia elata (G. elata) by hot-water extraction, ethanol precipitation, and membrane separator. GEP-1, which has a molecular weight of 20.1 kDa, contains a polysaccharide framework comprised of only glucose. Methylation and NMR analysis showed that GEP-1 contained 1,3,6-linked-α-Glcp, 1,4-linked-α-Glcp, 1,4-linked-β-Glcp and 1,4,6-linked-α-Glcp. Interestingly, GEP-1 contained citric acid and repeating p-hydroxybenzyl alcohol as one branch. Furthermore, a bioactivity test showed that GEP-1 could significantly promote the growth of Akkermansia muciniphila (A. muciniphila) and Lacticaseibacillus paracasei (L.paracasei) strains. These results implied that GEP-1 might be useful for human by modulating gut microbiota.

scholarly journals Transcriptome Reveals Roles of Lignin-Modifying Enzymes and Abscisic Acid in the Symbiosis of Mycena and Gastrodia elata

2021 ◽  
Vol 22 (12) ◽  
pp. 6557
Author(s):  
Li-Ying Ren ◽  
Heng Zhao ◽  
Xiao-Ling Liu ◽  
Tong-Kai Zong ◽  
Min Qiao ◽  
...  
Keyword(s):  
Biological Activity ◽  
Abscisic Acid ◽  
Lignin Degradation ◽  
Molecular Mechanisms ◽  
Receptor Protein ◽  
Gastrodia Elata ◽  
Upregulated Genes ◽  
Aba Biosynthesis ◽  
Seed Coats

Gastrodia elata is a well-known medicinal and heterotrophic orchid. Its germination, limited by the impermeability of seed coat lignin and inhibition by abscisic acid (ABA), is triggered by symbiosis with fungi such as Mycena spp. However, the molecular mechanisms of lignin degradation by Mycena and ABA biosynthesis and signaling in G. elata remain unclear. In order to gain insights into these two processes, this study analyzed the transcriptomes of these organisms during their dynamic symbiosis. Among the 25 lignin-modifying enzyme genes in Mycena, two ligninolytic class II peroxidases and two laccases were significantly upregulated, most likely enabling Mycena hyphae to break through the lignin seed coats of G. elata. Genes related to reduced virulence and loss of pathogenicity in Mycena accounted for more than half of annotated genes, presumably contributing to symbiosis. After coculture, upregulated genes outnumbered downregulated genes in G. elata seeds, suggesting slightly increased biological activity, while Mycena hyphae had fewer upregulated than downregulated genes, indicating decreased biological activity. ABA biosynthesis in G. elata was reduced by the downregulated expression of 9-cis-epoxycarotenoid dioxygenase (NCED-2), and ABA signaling was blocked by the downregulated expression of a receptor protein (PYL12-like). This is the first report to describe the role of NCED-2 and PYL12-like in breaking G. elata seed dormancy by reducing the synthesis and blocking the signaling of the germination inhibitor ABA. This study provides a theoretical basis for screening germination fungi to identify effective symbionts and for reducing ABA inhibition of G. elata seed germination.

scholarly journals First Report of Soft Rot Associated With Trichoderma hamatum in Gastrodia elata

Plant Disease ◽  
2017 ◽  
Vol 101 (6) ◽  
pp. 1048-1048 ◽  
Author(s):  
M. Han ◽  
M. N. Choi ◽  
H. R. Lee ◽  
E. J. Park
Keyword(s):  
Soft Rot ◽  
Gastrodia Elata ◽  
First Report ◽  
Trichoderma Hamatum

2,4-Bis(4-hydroxybenzyl) phenol from Gastrodia elata

1995 ◽  
Vol 39 (5) ◽  
pp. 1247-1248 ◽  
Author(s):  
N Noda
Keyword(s):  
Gastrodia Elata

scholarly journals Transcriptomic analyses reveal clathrin-mediated endocytosis involved in symbiotic seed germination of Gastrodia elata

2017 ◽  
Vol 58 (1) ◽  
Author(s):  
Xu Zeng ◽  
Yuanyuan Li ◽  
Hong Ling ◽  
Sisi Liu ◽  
Mengmeng Liu ◽  
...  
Keyword(s):  
Seed Germination ◽  
Gastrodia Elata ◽  
Symbiotic Seed Germination

New insights into the brain protein metabolism of Gastrodia elata-treated rats by quantitative proteomics

2012 ◽  
Vol 75 (8) ◽  
pp. 2468-2479 ◽  
Author(s):  
Arulmani Manavalan ◽  
Lin Feng ◽  
Siu Kwan Sze ◽  
Jiang-Miao Hu ◽  
Klaus Heese
Keyword(s):  
Protein Metabolism ◽  
Quantitative Proteomics ◽  
Brain Protein ◽  
Gastrodia Elata ◽  
The Brain
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