Ionic liquid as an effective solvent for cell wall deconstructing through astaxanthin extraction from Haematococcus pluvialis

2018 ◽  
Vol 54 (2) ◽  
pp. 583-590 ◽  
Author(s):  
Zhi-Wei Liu ◽  
Zhou Yue ◽  
Xin-An Zeng ◽  
Jun-Hu Cheng ◽  
Rana Muhammad Aadil
Keyword(s):  
Cell Wall ◽  
Ionic Liquid ◽  
Haematococcus Pluvialis ◽  
Astaxanthin Extraction

Novel astaxanthin extraction from Haematococcus pluvialis using cell permeabilising ionic liquids

2016 ◽  
Vol 18 (5) ◽  
pp. 1261-1267 ◽  
Author(s):  
Rupali K. Desai ◽  
Mathieu Streefland ◽  
Rene H. Wijffels ◽  
Michel H. M. Eppink
Keyword(s):  
Cell Wall ◽  
Ionic Liquid ◽  
Ionic Liquids ◽  
Ethyl Acetate ◽  
Haematococcus Pluvialis ◽  
Liquid Solution ◽  
Mechanical Disruption ◽  
Pre Treatment ◽  
Ionic Liquid Solution ◽  
Astaxanthin Extraction

Pre-treatment of H. pluvialis with an aqueous ionic liquid solution permeabilises the cell wall and astaxanthin can be subsequently extracted with ethyl acetate without mechanical disruption.

scholarly journals Techno-economic analysis of a new downstream process for the production of astaxanthin from the microalgae Haematococcus pluvialis

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Andreas Bauer ◽  
Mirjana Minceva
Keyword(s):  
Cell Wall ◽  
Economic Analysis ◽  
Ethyl Acetate ◽  
Net Present Value ◽  
Haematococcus Pluvialis ◽  
Light Stress ◽  
Downstream Process ◽  
Cell Wall Disruption ◽  
Cyst Cells ◽  
Astaxanthin Extraction

AbstractThe biotechnological production of the carotenoid astaxanthin is done with the microalgae Haematococcus pluvialis (H. pluvialis). Under nutrient deficiency and light stress, H. pluvialis accumulates astaxanthin intracellularly and forms a resistant cyst cell wall that impedes direct astaxanthin extraction. Therefore, a complex downstream process is required, including centrifugation, mechanical cell wall disruption, drying, and supercritical extraction of astaxanthin with CO2. In this work, an alternative downstream process based on the direct extraction of astaxanthin from the algal broth into ethyl acetate using a centrifugal partition extractor (CPE) was developed. A mechanical cell wall disruption or germination of the cysts was carried out to make astaxanthin accessible to the solvent. Zoospores containing astaxanthin are released when growth conditions are applied to cyst cells, from which astaxanthin can directly be extracted into ethyl acetate. Energy-intensive unit operations such as spray-drying and extraction with supercritical CO2 can be replaced by directly extracting astaxanthin into ethyl acetate. Extraction yields of 85% were reached, and 3.5 g of oleoresin could be extracted from 7.85 g homogenised H. pluvialis biomass using a CPE unit with 244 mL column volume. A techno-economic analysis was done for a hypothetical H. pluvialis production facility with an annual biomass output of 8910 kg. Four downstream scenarios were examined, comparing the novel process of astaxanthin extraction from homogenised cyst cells and germinated zoospores via CPE extraction with the conventional industrial process using in-house or supercritical CO2 extraction via an external service provider. After 10 years of operation, the highest net present value (NPV) was determined for the CPE extraction from germinated zoospores.

scholarly journals Relationship between sugarcane culm and leaf biomass composition and saccharification efficiency

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
K. Hodgson-Kratky ◽  
G. Papa ◽  
A. Rodriguez ◽  
V. Stavila ◽  
B. Simmons ◽  
...  
Keyword(s):  
Cell Wall ◽  
Ionic Liquid ◽  
Lignocellulosic Biomass ◽  
Plant Cell Wall ◽  
Biomass Composition ◽  
Leaf Biomass ◽  
Dilute Acid ◽  
Sugarcane Varieties ◽  
Saccharification Efficiency ◽  
G Ratio

Abstract Background Lignocellulosic biomass is recognized as a promising renewable feedstock for the production of biofuels. However, current methods for converting biomass into fermentable sugars are considered too expensive and inefficient due to the recalcitrance of the secondary cell wall. Biomass composition can be modified to create varieties that are efficiently broken down to release cell wall sugars. This study focused on identifying the key biomass components influencing plant cell wall recalcitrance that can be targeted for selection in sugarcane, an important and abundant source of biomass. Results Biomass composition and the amount of glucan converted into glucose after saccharification were measured in leaf and culm tissues from seven sugarcane genotypes varying in fiber composition after no pretreatment and dilute acid, hydrothermal and ionic liquid pretreatments. In extractives-free sugarcane leaf and culm tissue, glucan, xylan, acid-insoluble lignin (AIL) and acid-soluble lignin (ASL) ranged from 20 to 32%, 15% to 21%, 14% to 20% and 2% to 4%, respectively. The ratio of syringyl (S) to guaiacyl (G) content in the lignin ranged from 1.5 to 2.2 in the culm and from 0.65 to 1.1 in the leaf. Hydrothermal and dilute acid pretreatments predominantly reduced xylan content, while the ionic liquid (IL) pretreatment targeted AIL reduction. The amount of glucan converted into glucose after 26 h of pre-saccharification was highest after IL pretreatment (42% in culm and 63.5% in leaf) compared to the other pretreatments. Additionally, glucan conversion in leaf tissues was approximately 1.5-fold of that in culm tissues. Percent glucan conversion varied between genotypes but there was no genotype that was superior to all others across the pretreatment groups. Path analysis revealed that S/G ratio, AIL and xylan had the strongest negative associations with percent glucan conversion, while ASL and glucan content had strong positive influences. Conclusion To improve saccharification efficiency of lignocellulosic biomass, breeders should focus on reducing S/G ratio, xylan and AIL content and increasing ASL and glucan content. This will be key for the development of sugarcane varieties for bioenergy uses.

scholarly journals Astaxanthin extraction from Haematococcus pluvialis using CO2-expanded ethanol

2014 ◽  
Vol 92 ◽  
pp. 75-83 ◽  
Author(s):  
Fabián A. Reyes ◽  
José A. Mendiola ◽  
Elena Ibañez ◽  
José M. del Valle
Keyword(s):  
Haematococcus Pluvialis ◽  
Astaxanthin Extraction
Sign in / Sign up

Export Citation Format

Share Document