1 H NMR and Raman spectroscopy of oils and extracts obtained from organic and conventional goji berries: yield, fatty acids, carotenoids and biological activities

2018 ◽  
Vol 54 (1) ◽  
pp. 282-290 ◽  
Author(s):  
Alessandra Cristina Pedro ◽  
Fabiane Bach ◽  
Ana Paula Stafussa ◽  
Leociley Rocha Alencar Menezes ◽  
Andersson Barison ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Raman Spectroscopy ◽  
Biological Activities ◽  
H Nmr ◽  
Goji Berries

scholarly journals Plant Extract Valorization of Melissa officinalis L. for Agroindustrial Purposes through Their Biochemical Properties and Biological Activities

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Mouna Souihi ◽  
Rayda Ben Ayed ◽  
Imen Trabelsi ◽  
Marwa Khammassi ◽  
Nadia Ben Brahim ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Biological Activities ◽  
Biochemical Properties ◽  
Major Constituent ◽  
Allelopathic Effect ◽  
Melissa Officinalis ◽  
Lemon Balm ◽  
The North ◽  
Germacrene D ◽  
Leaf Essential Oil

Lemon balm (Melissa officinalis L.) is one of the rare medicinal plants in Tunisia. It was found only in two sites in the north of Tunisia with a small number of plants. The study of germination under the NaCl and PEG effect showed that Tunisian lemon balm seeds were sensitive to saline and osmotic stress. Morphological and biochemical characterizations of Tunisian M. officinalis were performed. Results showed that the Tunisian populations presented plants with long, broad leaves and weak branching. The major constituent in leaf essential oil was germacrene-D with a percentage ranging from 29.17 to 24.6%, and the major fatty acids were polyunsaturated fatty acids, linoleic acid, ranging from 73.93 to 66.74%. The phenolic content of M. officinalis extract varied significantly among origins which could explain the high variation in antiradical scavenging activity. The evaluation of allelopathic activities showed that the extract of the lemon balm leaves presented an allelopathic effect with the majority of the tested seeds.

Synthesis and characterization of a new series of thiadiazole derivatives as potential anticancer agents

2020 ◽  
Vol 26 (1) ◽  
pp. 6-13 ◽  
Author(s):  
Ulviye Acar Çevik ◽  
Derya Osmaniye ◽  
Serkan Levent ◽  
Begüm Nurpelin Sağlik ◽  
Betül Kaya Çavuşoğlu ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Biological Activities ◽  
Cancer Cell Line ◽  
Chemotherapeutic Agents ◽  
Anticancer Activities ◽  
Normal Tissues ◽  
H Nmr ◽  
Wide Range ◽  
Thiadiazole Derivatives ◽  
Low Efficiency

AbstractCancer is one of the most common causes of death in the world. Despite the importance of combating cancer in healthcare systems and research centers, toxicity in normal tissues and the low efficiency of anticancer drugs are major problems in chemotherapy. Nowadays the aim of many medical research projects is to discover new safer and more effective anticancer agents. 1,3,4-Thiadiazole compounds are important fragments in medicinal chemistry because of their wide range of biological activities, including anticancer activities. The aim of this study was to determine the capacity of newly synthesized 1,3,4-thiadiazole compounds as chemotherapeutic agents. The structures of the obtained compounds were elucidated using 1H-NMR, 13C-NMR and mass spectrometry. Although the thiadiazole derivatives did not prove to be significantly cytotoxic to the tumour tissue cultures, compound 4i showed activity against the C6 rat brain cancer cell line (IC50 0.097 mM) at the tested concentrations.

scholarly journals An Alternative Approach for the Synthesis of Sulfoquinovosyldiacylglycerol

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4275
Author(s):  
Tobias Sitz ◽  
Hendrik Domey ◽  
Judith Fischer ◽  
Sascha Rohn
Keyword(s):  
Fatty Acids ◽  
Functional Foods ◽  
Unsaturated Fatty Acids ◽  
Biological Activities ◽  
Saturated Fatty Acids ◽  
Diverse Range ◽  
Natural Sources ◽  
Model Studies ◽  
Alternative Approach ◽  
Increasing Demand

Sulfoquinovosyldiacylglycerol (SQDG) is a glycolipid ubiquitously found in photosynthetically active organisms. It has attracted much attention in recent years due to its biological activities. Similarly, the increasing demand for vegan and functional foods has led to a growing interest in micronutrients such as sulfolipids and their physiological influence on human health. To study this influence, reference materials are needed for developing new analytical methods and providing enough material for model studies on the biological activity. However, the availability of these materials is limited by the difficulty to isolate and purify sulfolipids from natural sources and the unavailability of chemical standards on the market. Consequently, an alternative synthetic route for the comprehensive preparation of sulfolipids was established. Here, the synthesis of a sulfolipid with two identical saturated fatty acids is described exemplarily. The method opens possibilities for the preparation of a diverse range of interesting derivatives with different saturated and unsaturated fatty acids.

scholarly journals Synthesis, Characterization, and Agricultural Biological Activities of 5-Fluoro-2-hydroxy Butyrophenone

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Fengli Xin ◽  
Chunhua Du ◽  
Gongjia Lan ◽  
Zhuanping Wu
Keyword(s):  
Lactuca Sativa ◽  
Biological Activities ◽  
Raw Material ◽  
Synthetic Approach ◽  
Target Compound ◽  
Echinochloa Crusgalli ◽  
Agricultural Plant ◽  
H Nmr ◽  
Valsa Mali ◽  
C Nmr

A novel synthetic approach towards 5-fluoro-2-hydroxy butyrophenone is reported. Using 4-fluorophenol as a raw material, the processes of etherification protection, Friedel-Crafts acylation and demethylation provide the target compound under mild conditions. The structure was characterized by the melting point and IR, MS,1H-NMR, and13C-NMR spectroscopy. The bioassay results indicate that the target compound exhibits potent antifungal activities againstValsa mali,Coniella dipodiella, and other agricultural plant fungi. The target compound also shows potent herbicidal activities forLactuca sativa, a dicotyledon, andEchinochloa crus-galli, a monocotyledon. The toxicity regression C50values of the compound againstValsa mali,Coniothyrium diplodiella,Lactuca sativaseedling, andEchinochloa crusgalliseedling were calculated by SPSS. The Hormesis effect for roots ofEchinochloa crusgalliwas confirmed.

scholarly journals Determination of Cyclopropane Fatty Acids in Food of Animal Origin by 1H NMR

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Veronica Lolli ◽  
Angela Marseglia ◽  
Gerardo Palla ◽  
Emanuela Zanardi ◽  
Augusta Caligiani
Keyword(s):  
Fatty Acids ◽  
Dairy Products ◽  
Cyclopropane Ring ◽  
Food Sources ◽  
Animal Origin ◽  
Animal Fats ◽  
H Nmr ◽  
Cyclopropane Fatty Acids ◽  
Food Of Animal Origin ◽  
Full Scan

Cyclopropane fatty acids (CPFAs) are unusual fatty acids of microbial origin, recently detected in milk and dairy products. CPFAs have been demonstrated to be interesting molecular markers for authentication of dairy products obtained without ensiled feeds. Moreover, they can also be recognized as a new secondary component of human diet. Information is lacking on the presence of cyclic fatty acids in other food sources. Cyclopropane fatty acids have been detected by GC-MS analysis in cheese and other animal fats in concentration ranging from 200 to 1000 mg/kg fat, but in some cases, the complex fatty acid profile and the possible presence of interfering peaks make the separation not straightforward and the quantification uneasy. Therefore, a new reliable 1H NMR method was developed to detect and measure CPFA content in different foods of animal origin, based on the detection of the characteristic signals of cyclopropane ring. The 1H NMR (600 MHz) method showed detection limits comparable with those of full scan GC-MS, and it allowed the identification and quantitation of the cyclopropane fatty acids in different foods.

Über Inhaltsstoffe von Euphorbiaceae, 8. Mitt. [1]. Triterpenester und Triterpenalkohole aus Euphorbia biglandulosa Desf. / Constituents of Euphorbiaceae, 8. Comm. [1]. Triterpen Esters and Triterpen Alcohols from Euphorbia biglandulosa Desf.

1985 ◽  
Vol 40 (4) ◽  
pp. 553-555 ◽  
Author(s):  
Gioacchino Falsone ◽  
Claudia Schneider
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
H Nmr ◽  
Acetic Acid Esters ◽  
Acid Esters

Fatty acids and acetic acid esters of lanosterol and cycloartenol and triterpens alcohols were isolated from the latex of Euphorbia biglandulosa Desf. The triterpenesters were identified as (E,Z)-2,4-decadienoyllanosterol (1a), (E,Z)-2,4-octadienoyllanosterol (1b), (E,Z)-2,4-oc- tadienoylcycloartenol (2a), (2E,4Z)-2,4,6-decatrienoyllanosterol (1c), (2E,4Z)-2,4,6-deca- trienoylcycloartenol (2b), lanosterylacetat (1d) and cycloartenylacetat (2c) by IR-, 1H-NMR- and MS-spectroscopy and hydrolysis. The triterpens alcohols were identified as butyrospermol, cyclo­artenol, 24-methylencycloartanol and obtusifoliol.

scholarly journals Role of caffeic and chlorogenic acid in the modulation of cellular fatty acid uptake

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Mirko Marino ◽  
Massimiliano Tucci ◽  
Valentina Taverniti ◽  
Patrizia Riso ◽  
Marisa Porrini ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Chlorogenic Acid ◽  
Phenolic Acids ◽  
Lipid Accumulation ◽  
Biological Activities ◽  
Fatty Acid Uptake ◽  
Cellular Fatty Acid ◽  
Bioactive Molecules ◽  
Acid Uptake

AbstractPolyphenols are bioactive molecules widely distributed in numerous foods such as fruits, vegetables, tea, coffee, cocoa and beverages. Their main classification include flavonoids (i.e. flavonols, flavones, flavanones, flavanols, anthocyanins, and isoflavones), non-flavonoids (i.e. lignans and stilbens) and phenolic acids (i.e. hydroxycinnamic and hydroxybenzoic acids)(1). Caffeic acid (CA) and chlorogenic acid (CGA; an ester of CA and quinic acid) are the major representatives of hydroxycinnamic acids. Accumulating evidence has demonstrated that CA and CGA may exert different biological activities, including antioxidant, anti-inflammatory, antidiabetic, and antihypertensive(2). Despite these promising and diverse anti-atherosclerotic actions, investigations addressing the effect of CA and CGA on atherogenesis are scarce.The present study evaluated the capacity of CA and CGA to reduce lipid accumulation in macrophages derived from monocytic THP-1 cells. THP-1-derived macrophages were incubated with fatty acids (500 μM oleic/palmitic acid, 2:1 ratio) and different concentrations (from 0.03 to 3 μM) of CA and CGA, alone or in combination. Lipid accumulation was quantified spectrophotometrically (excitation: 544 nm, emission: 590 nm) with the fluorescent dye, Nile red. The fold increase compared to the control (without fatty acids) was calculated. In addition, the expression of several transcription factors including peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein (CEBP), as potential mechanisms involved in the regulation of lipid accumulation, was evaluated by real time PCR.Analysis of variance (ANOVA) was used to assess the effect of the different concentrations of CA and CGA on lipid accumulation in THP-1 macrophages following stimulation with FA.The preliminary results obtained have shown a significant increase in lipid accumulation following fatty acid exposure (p < 0.0001). Incubation with CA and CGA did not reduce lipid accumulation in THP-1 derived macrophages, while the combination of CA + CGA at 0.03, 0.3 and 3 μM (p < 0.01) decreased cellular fatty acid uptake at all concentrations tested by -28%, -32%, -23%, respectively. An apparent modulation of the transcriptional activity of PPARγ, but not CEBP, was observed following the combination of phenolic acids.In conclusion, the incubation of CA + CGA at physiologically relevant concentrations, but not the single compounds, seem to reduce the uptake of fatty acids in THP-1-derived macrophages. Further experiments are ongoing in order to confirm the findings obtained and to better identify the mechanisms of action involved in the reduction of lipid accumulation as a key phenomenon of atherogenesis.

scholarly journals Characterization and Biological Activities of Seed Oil Extracted from Berberis dasystachya Maxim. by the Supercritical Carbon Dioxide Extraction Method

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1836
Author(s):  
Lijuan Han ◽  
Qingqing Han ◽  
Yongjing Yang ◽  
Honglun Wang ◽  
ShuLin Wang ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Supercritical Carbon Dioxide ◽  
Extraction Method ◽  
Unsaturated Fatty Acids ◽  
Biological Activities ◽  
Maximum Yield ◽  
Dependent Manner ◽  
Supercritical Carbon

Characterization of the structure and pharmacological activity of Berberis dasystachya Maxim., a traditional Tibetan medicinal and edible fruit, has not yet been reported. In this study, central composite design (CCD) combined with response surface methodology (RSM) was applied to optimize the extraction conditions of B. dasystachya oil (BDSO) using the supercritical carbon dioxide (SC-CO2) extraction method, and the results were compared with those obtained by the petroleum ether extraction (PEE) method. The chemical characteristics of BDSO were analyzed, and its antioxidant activity and in vitro cellular viability were studied by DPPH, ABTS, reducing power assay, and MTT assay. The results showed that the maximum yield of 12.54 ± 0.56 g/100 g was obtained at the optimal extraction conditions, which were: pressure, 25.00 MPa; temperature 59.03 °C; and CO2 flow rate, 2.25 SL/min. The Gas chromatography (GC) analysis results showed that BDSO extracted by the SC-CO2 method had higher contents of unsaturated fatty acids (85.62%) and polyunsaturated fatty acids (57.90%) than that extracted by the PEE method. The gas chromatography used in conjunction with ion mobility spectrometry (GC–IMS) results showed that the main volatile compounds in BDSO were aldehydes and esters. BDSO also exhibited antioxidant ability in a dose-dependent manner. Moreover, normal and cancer cells incubated with BDSO had survival rates of more than 85%, which indicates that BDSO is not cytotoxic. Based on these results, the BDSO extracted by the SC-CO2 method could potentially be used in other applications, e.g., those that involve using berries of B. dasystachya.

scholarly journals Ratiometric analysis using Raman spectroscopy as a powerful predictor of structural properties of fatty acids

2018 ◽  
Vol 5 (12) ◽  
pp. 181483 ◽  
Author(s):  
Lauren E. Jamieson ◽  
Angela Li ◽  
Karen Faulds ◽  
Duncan Graham
Keyword(s):  
Fatty Acids ◽  
Raman Spectroscopy ◽  
Biological Sample ◽  
Ex Vivo ◽  
Degree Of Saturation ◽  
Specific Information ◽  
Analytical Technique ◽  
Starting Point

Raman spectroscopy has been used extensively for the analysis of biological samples in vitro , ex vivo and in vivo . While important progress has been made towards using this analytical technique in clinical applications, there is a limit to how much chemically specific information can be extracted from a spectrum of a biological sample, which consists of multiple overlapping peaks from a large number of species in any particular sample. In an attempt to elucidate more specific information regarding individual biochemical species, as opposed to very broad assignments by species class, we propose a bottom-up approach beginning with a detailed analysis of pure biochemical components. Here, we demonstrate a simple ratiometric approach applied to fatty acids, a subsection of the lipid class, to allow the key structural features, in particular degree of saturation and chain length, to be predicted. This is proposed as a starting point for allowing more chemically and species-specific information to be elucidated from the highly multiplexed spectrum of multiple overlapping signals found in a real biological sample. The power of simple ratiometric analysis is also demonstrated by comparing the prediction of degree of unsaturation in food oil samples using ratiometric and multivariate analysis techniques which could be used for food oil authentication.

Synthesis and Characterization of a New Coumarin-1,2,3-Triazole Diad

2014 ◽  
Vol 618 ◽  
pp. 170-174
Author(s):  
Hong Qi Li ◽  
Xiu Juan Tian ◽  
Zhen Chen ◽  
Ding Xiao ◽  
Ning Fang ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Biological Activities ◽  
Antitubercular Activity ◽  
Synthesis And Characterization ◽  
Target Compound ◽  
H Nmr ◽  
Mild Conditions ◽  
Ideal Approach ◽  
C Nmr

A new coumarin-1,2,3-triazole diad was designed and synthesized by Pechmann-Duisberg reaction and click chemistry. The three-step synthetic route to the target compound afforded an overall yield of 56%. The structures of the synthesized compounds were characterized by 1H NMR, 13C NMR, IR and mass spectrum. The click chemistry provided an ideal approach for synthesis under mild conditions of coumarin-1,2,3-triazole diads which may exhibit excellent biological activities such as antitubercular activity.

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