Antihypertensive properties of aqueous extracts of vegetable leaf-fortified bread after oral administration to spontaneously hypertensive rats

2018 ◽  
Vol 53 (7) ◽  
pp. 1705-1716 ◽  
Author(s):  
Adeola M. Alashi ◽  
Kehinde A. Taiwo ◽  
Durodoluwa Oyedele ◽  
Odunayo C. Adebooye ◽  
Rotimi E. Aluko
Keyword(s):  
Oral Administration ◽  
Spontaneously Hypertensive Rats ◽  
Aqueous Extracts ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Antihypertensive Properties

scholarly journals Evidence that Nitric Oxide is Involved in the Blood Pressure Lowering Effect of the Peptide AVFQHNCQE in Spontaneously Hypertensive Rats

Nutrients ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 225 ◽  
Author(s):  
Anna Mas-Capdevila ◽  
Lisard Iglesias-Carres ◽  
Anna Arola-Arnal ◽  
Gerard Aragonès ◽  
Amaya Aleixandre ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Oral Administration ◽  
Spontaneously Hypertensive Rats ◽  
Protein Hydrolysate ◽  
Antioxidant Properties ◽  
Hypertensive Rats ◽  
Blood Pressure Lowering Effect ◽  
Spontaneously Hypertensive ◽  
Lowering Effect

AVFQHNCQE is an antihypertensive nonapeptide obtained from a chicken foot protein hydrolysate. The present study aims to investigate the mechanisms involved in its blood pressure (BP)-lowering effect. Male (17–20 weeks old) spontaneously hypertensive rats (SHR) were used in this study. Rats were divided into two groups and orally administered water or 10 mg/kg body weight (bw) AVFQHNCQE. One hour post-administration, animals of both groups were intra-peritoneally treated with 1 mL of saline or with 1 mL of saline containing 30 mg/kg bw Nω-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) synthesis, or with 1 mL of saline containing 5 mg/kg bw indomethacin, which is an inhibitor of prostacyclin synthesis (n = 6 per group). Systolic BP was recorded before oral administration and six hours after oral administration. In an additional experiment, SHR were administered water or 10 mg/kg bw AVFQHNCQE (n = 6 per group) and sacrificed six hours post-administration to study the mechanisms underlying the peptide anti-hypertensive effect. Moreover, the relaxation caused by AVFQHNCQE in isolated aortic rings from Sprague-Dawley rats was evaluated. The BP-lowering effect of the peptide was not changed after indomethacin administration but was completely abolished by L-NAME, which demonstrates that its anti-hypertensive effect is mediated by changes in endothelium-derived NO availability. In addition, AVFQHNCQE administration downregulated aortic gene expression of the vasoconstrictor factor endothelin-1 and the endothelial major free radical producer NADPH. Moreover, while no changes in plasma ACE activity were observed after its administration, liver GSH levels were higher in the peptide-treated group than in the water group, which demonstrates that AVFQHNCQE presents antioxidant properties.

Fermented rice bran extract improves blood pressure and glucose in stroke-prone spontaneously hypertensive rats

2019 ◽  
Vol 49 (5) ◽  
pp. 844-853 ◽  
Author(s):  
Ardiansyah ◽  
Wahyudi David ◽  
Dody Dwi Handoko ◽  
Bram Kusbiantoro ◽  
Slamet Budijanto ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Oral Administration ◽  
Rice Bran ◽  
Spontaneously Hypertensive Rats ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Hypertensive Rats ◽  
Content Type ◽  
Spontaneously Hypertensive

PurposeThe purpose of this study was to evaluate thein vitroandin vivoeffects of three varieties of Indonesian fermented rice bran (RB) (Inpari 6, Inpari 30 and Inpara 1).Design/methodology/approachThree types of RB were fermented usingRhizopus oligosporus. The total phenolic content (TPC) was determined using the Folin–Ciocalteau method, and antioxidant activity was analyzed by measuring the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity (RSA). Forin vivoanalyses, one week after acclimatization, stroke-prone spontaneously hypertensive rats (n= 4, 12 weeks of age) were divided into two groups and orally administered fermented RB (40 mg/kg body weight) or distilled water as a control after 16 h of fasting. Blood pressure (BP) was measured before and 2, 4 and 6 h after administration with a BP monitor without warming. Six days later, the rats were subject to the same procedure and sacrificed after 6 h of oral administration. Blood was collected and the plasma was separated to measure nitric oxide, glucose and insulin levels.FindingsThe highest TPC and RSA of fermented RB were obtained from Inpari 30 after incubation for 72 h (260.33 ± 0.39 mg GAE/100 g dry basis and 83.71 ± 0.61 per cent), respectively. Furthermore, single oral administration of fermented RB improved BP (p< 0.05) and glucose metabolism after 6 h of administration (p< 0.05).Originality/valueThis is the first study to evaluate the effects of fermented RB on improving high BP and glucose profiles by using a spontaneously hypertensive animal model.

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