Development of a spectroscopic method to determine the content of free radical scavenging compounds and oxidation products in thermally oxidised oils

2016 ◽  
Vol 51 (11) ◽  
pp. 2424-2432 ◽  
Author(s):  
JuHee Song ◽  
Eun Yeong Jang ◽  
Mi-Ja Kim ◽  
Young-Jun Kim ◽  
JaeHwan Lee
Keyword(s):  
Free Radical ◽  
Spectroscopic Method ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Oxidation Products ◽  
Radical Scavenging Compounds

scholarly journals Antioxidant Property of Aerial Parts and Root ofPhyllanthus fraternusWebster, an Important Medicinal Plant

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Richa Upadhyay ◽  
Jitendra Kumar Chaurasia ◽  
Kavindra Nath Tiwari ◽  
Karuna Singh
Keyword(s):  
Free Radical ◽  
Aerial Part ◽  
Radical Scavenging Activity ◽  
Spectroscopic Method ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Aerial Parts

In present study free radical scavenging potential of aerial parts and root ofPhyllanthus fraternuswas investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC50of aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis ofFe3+-Fe2+transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential. Thus,P. fraternusextract can be used as potent natural antioxidant.

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