Effect of chloride ions in Tris buffer solution on bioactive glass apatite mineralization

2017 ◽  
Vol 8 (4) ◽  
pp. 438-449 ◽  
Author(s):  
Gloria Kirste ◽  
Juliane Brandt-Slowik ◽  
Christian Bocker ◽  
Michael Steinert ◽  
Reinhard Geiss ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Chloride Ions ◽  
Tris Buffer ◽  
Buffer Solution ◽  
Tris Buffer Solution

scholarly journals A rapidly-dissolving silica-silver bioactive glass for cariostatic applications

2021 ◽  
Author(s):  
Saad Arshad
Keyword(s):  
Oral Cavity ◽  
Antibacterial Effect ◽  
Surface Hardness ◽  
Tris Buffer ◽  
Bacterial Strains ◽  
Buffer Solution ◽  
Solution Ph ◽  
Before And After ◽  
Knoop Microhardness ◽  
Tris Buffer Solution

In this study, bioactive glasses were designed for the purposes of reducing the incidence of dental caries and lesion formation by supplying the teeth with therapeutic ions which may support remineralization and provide an antibacterial effect against oral cavity bacteria. Three glasses were synthesised through the melt quench method: Si-Control (SiO2-CaO-P2O5-Na2O), Si-02 and Si-05, where 0.2% and 0.5% Ag2O were substituted, respectively, for SiO2 in the control glass. The glasses were then ground, characterized and dissolved in tris buffer solution (pH=7.30) for 6, 12 and 24 hours, with the pH rise of the solution being recorded (7.48 for Si-Control, and 7.66 for both Si-02 and Si-05 after 24 hours) and the ions that were released into the tris buffer solution quantified. Samples of each glass were subsequently embedded into non-fluoridated toothpaste and samples of the paste were used to brush resin-mounted lamb molars after a 1.0M HCl overnight demineralization challenge. Knoop microhardness measurements were recorded before and after brushing to determine the presence of remineralization on the surface of the teeth (Percent Surface Hardness Loss of 37%, 35% and 34% for Si-Control, Si-02 and Si-05 respectively after 24 hours). Four oral cavity bacterial strains were isolated through swabs of the inner cheek, gums and teeth surfaces of three volunteers, and placed on agar discs. 0.5g of each glass were placed onto the discs and the resultant inhibition zones were measured after 6, 12 and 24 hours. Si-05 consistently performed better than Si-02 on all strains and timeframes, while Si-Control exhibited no antibacterial effect at any time point.

scholarly journals A rapidly-dissolving silica-silver bioactive glass for cariostatic applications

2021 ◽  
Author(s):  
Saad Arshad
Keyword(s):  
Oral Cavity ◽  
Antibacterial Effect ◽  
Surface Hardness ◽  
Tris Buffer ◽  
Bacterial Strains ◽  
Buffer Solution ◽  
Solution Ph ◽  
Before And After ◽  
Knoop Microhardness ◽  
Tris Buffer Solution

In this study, bioactive glasses were designed for the purposes of reducing the incidence of dental caries and lesion formation by supplying the teeth with therapeutic ions which may support remineralization and provide an antibacterial effect against oral cavity bacteria. Three glasses were synthesised through the melt quench method: Si-Control (SiO2-CaO-P2O5-Na2O), Si-02 and Si-05, where 0.2% and 0.5% Ag2O were substituted, respectively, for SiO2 in the control glass. The glasses were then ground, characterized and dissolved in tris buffer solution (pH=7.30) for 6, 12 and 24 hours, with the pH rise of the solution being recorded (7.48 for Si-Control, and 7.66 for both Si-02 and Si-05 after 24 hours) and the ions that were released into the tris buffer solution quantified. Samples of each glass were subsequently embedded into non-fluoridated toothpaste and samples of the paste were used to brush resin-mounted lamb molars after a 1.0M HCl overnight demineralization challenge. Knoop microhardness measurements were recorded before and after brushing to determine the presence of remineralization on the surface of the teeth (Percent Surface Hardness Loss of 37%, 35% and 34% for Si-Control, Si-02 and Si-05 respectively after 24 hours). Four oral cavity bacterial strains were isolated through swabs of the inner cheek, gums and teeth surfaces of three volunteers, and placed on agar discs. 0.5g of each glass were placed onto the discs and the resultant inhibition zones were measured after 6, 12 and 24 hours. Si-05 consistently performed better than Si-02 on all strains and timeframes, while Si-Control exhibited no antibacterial effect at any time point.

scholarly journals Novel fluoride and strontium-containing bioactive glasses for dental varnishes-design and bioactivity in Tris buffer solution

2019 ◽  
Vol 503-504 ◽  
pp. 120-130 ◽  
Author(s):  
Thaer Jaber Al-Khafaji ◽  
Ferranti Wong ◽  
Padhraig S. Fleming ◽  
Natalia Karpukhina ◽  
Robert Hill
Keyword(s):  
Tris Buffer ◽  
Buffer Solution ◽  
Bioactive Glasses ◽  
Tris Buffer Solution

Resurveying the Tris buffer solution: The specific interaction between tris(hydroxymethyl)aminomethane and lysozyme

2008 ◽  
Vol 378 (2) ◽  
pp. 144-150 ◽  
Author(s):  
Li Quan ◽  
Dengguo Wei ◽  
Xiaolu Jiang ◽  
Yang Liu ◽  
Zhiyu Li ◽  
...  
Keyword(s):  
Specific Interaction ◽  
Tris Buffer ◽  
Buffer Solution ◽  
Tris Buffer Solution

scholarly journals Ion release from SrO-CaO-TiO2-P2O5 glasses in Tris buffer solution

2009 ◽  
Vol 117 (1369) ◽  
pp. 935-938 ◽  
Author(s):  
Sungho LEE ◽  
Akiko OBATA ◽  
Toshihiro KASUGA
Keyword(s):  
Tris Buffer ◽  
Ion Release ◽  
Buffer Solution ◽  
Tris Buffer Solution

Thermal Study of Lysozyme Binding with β-Cyclodextrin

2011 ◽  
Vol 110-116 ◽  
pp. 1966-1969 ◽  
Author(s):  
Gholamreza Rezaei Behbehani ◽  
Lyla Barzegar
Keyword(s):  
Isothermal Titration Calorimetry ◽  
Thermal Study ◽  
Tris Buffer ◽  
Titration Calorimetry ◽  
Solvation Model ◽  
Buffer Solution ◽  
Tris Buffer Solution

Effects of β-cyclodextrin, βCD, on refolding of lysozyme was investigated at pH 12 employing isothermal titration calorimetry (ITC) at 300K in 30mM Tris buffer solution. βCD was employed as an anti-aggregation agent and the heats obtained for lysozyme+βCD interactions are reported and analyzed in terms of the extended solvation model. It was indicated that there are two sets of identical and non-cooperative sites for βCD.

In Vitro Degradation Studies of Calcium Polyphosphate Ceramics Prepared by Controlled Degree of Polymerization and Crystallization

2005 ◽  
Vol 288-289 ◽  
pp. 553-556 ◽  
Author(s):  
Kai Qiu ◽  
Chang Xiu Wang ◽  
Qi Zhang ◽  
Xin Chen ◽  
Hongfa Su
Keyword(s):  
Weight Loss ◽  
Citric Acid ◽  
Degree Of Polymerization ◽  
Tris Buffer ◽  
Polycondensation Reaction ◽  
Buffer Solution ◽  
In Vitro Degradation ◽  
Calcium Polyphosphate ◽  
Tris Buffer Solution

Porous calcium polyphosphate(CPP) have been shown to promise for tissue engineered implant application. The process from Ca(H2PO4)2 to CPP, as a polycondensation reaction, has been researched to evaluate the number average degree of polymerization. CPP with different degree of polymerization were prepared by controlling the calcining time. Amorphous and different crystalline CPP were prepared by the quenching from the melt and crystallization of amorphous CPP. Two specimens were soaked into citric acid and tris-buffer solution for 1 to 30 days. The weight loss of CPP with different degree of polymerizations and crystal types are different. With the increasing of degree of polymerization, the weight loss during the degradation is decreasing, contrarily the strength of CPP is increasing. The degradation velocity of amorphous CPP, α-CPP,β-CPP and γ-CPP with the same degree of polymerization decreased in turn at the same period. The full weight loss period of CPP can be changed between 17 days and more than 1 year. The degradation and deposition was faster in the citric acid than the tris-buffer solution.

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