In vitro skin model for characterization of sunscreen substantivity upon perspiration

2021 ◽  
Author(s):  
Fatemeh Keshavarzi ◽  
Nina Østergaard Knudsen ◽  
Jonathan R. Brewer ◽  
Morten F. Ebbesen ◽  
Niloufarsadat Mirmahdi Komjani ◽  
...  
Keyword(s):  
Skin Model ◽  
In Vitro Skin Model

Characterization of a new full thickness in vitro skin model

2021 ◽  
Author(s):  
Christelle Plaza ◽  
Celine Meyrignac ◽  
Jean Marie Botto ◽  
Christophe Capallere
Keyword(s):  
Full Thickness ◽  
Skin Model ◽  
In Vitro Skin Model

Fabrication and Characterization of in vitro 2D Skin Model - An attempt to establish Scaffold for Tissue Engineering

2021 ◽  
Author(s):  
Rajesh Pandiyan ◽  
Abimanyu Sugumaran ◽  
Sumathi Samiappan ◽  
Parameshwaran Sengottaiyan ◽  
Sivasankaran Ayyaru ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Skin Model ◽  
Fabrication And Characterization

scholarly journals Preliminary Study of In Vitro Three-Dimensional Skin Model Using an Ovine Collagen Type I Sponge Seeded with Co-Culture Skin Cells: Submerged versus Air-Liquid Interface Conditions

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2784
Author(s):  
Mh Busra Fauzi ◽  
Zahra Rashidbenam ◽  
Aminuddin Bin Saim ◽  
Ruszymah Binti Hj Idrus
Keyword(s):  
Collagen Type ◽  
Three Dimensional ◽  
Collagen Type I ◽  
Liquid Interface ◽  
Type I ◽  
Skin Model ◽  
Skin Cells ◽  
Air Liquid Interface ◽  
In Vitro Skin Model

Three-dimensional (3D) in vitro skin models have been widely used for cosmeceutical and pharmaceutical applications aiming to reduce animal use in experiment. This study investigate capability of ovine tendon collagen type I (OTC-I) sponge suitable platform for a 3D in vitro skin model using co-cultured skin cells (CC) containing human epidermal keratinocytes (HEK) and human dermal fibroblasts (HDF) under submerged (SM) and air-liquid interface (ALI) conditions. Briefly, the extracted OTC-I was freeze-dried and crosslinked with genipin (OTC-I_GNP) and carbodiimide (OTC-I_EDC). The gross appearance, physico-chemical characteristics, biocompatibility and growth profile of seeded skin cells were assessed. The light brown and white appearance for the OTC-I_GNP scaffold and other groups were observed, respectively. The OTC-I_GNP scaffold demonstrated the highest swelling ratio (~1885%) and water uptake (94.96 ± 0.14%). The Fourier transformation infrared demonstrated amide A, B and I, II and III which represent collagen type I. The microstructure of all fabricated sponges presented a similar surface roughness with the presence of visible collagen fibers and a heterogenous porous structure. The OTC-I_EDC scaffold was more toxic and showed the lowest cell attachment and proliferation as compared to other groups. The micrographic evaluation revealed that CC potentially formed the epidermal- and dermal-like layers in both SM and ALI that prominently observed with OTC-I_GNP compared to others. In conclusion, these results suggest that OTC_GNP could be used as a 3D in vitro skin model under ALI microenvironment.

Can the prevalidated in vitro skin model phototoxicity test be upgraded to quantify phototoxic potency of topical phototoxins?

2007 ◽  
Vol 172 ◽  
pp. S81-S82 ◽  
Author(s):  
Helena Kandárová ◽  
Kristína Kejlová ◽  
Dagmar Jírová ◽  
Hana Bendová ◽  
Julian Tharmann ◽  
...  
Keyword(s):  
Skin Model ◽  
In Vitro Skin Model

Characterization of Rabbit Ear Skin as a Skin Model for in vitro Transdermal Permeation Experiments: Histology, Lipid Composition and Permeability

2008 ◽  
Vol 21 (4) ◽  
pp. 218-226 ◽  
Author(s):  
S. Nicoli ◽  
C. Padula ◽  
V. Aversa ◽  
B. Vietti ◽  
P.W. Wertz ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Skin Model ◽  
Transdermal Permeation ◽  
Rabbit Ear

Construction of 3D multicellular microfluidic chip for an in vitro skin model

2017 ◽  
Vol 19 (2) ◽  
Author(s):  
Sojin Lee ◽  
Seon-Pil Jin ◽  
Yeon Kyung Kim ◽  
Gun Yong Sung ◽  
Jin Ho Chung ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Skin Model ◽  
In Vitro Skin Model
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