scholarly journals Massively parallel sequencing analysis of benign melanocytic naevi

2019 ◽  
Vol 75 (1) ◽  
pp. 29-38 ◽  
Author(s):  
John R Lozada ◽  
Felipe C Geyer ◽  
Pier Selenica ◽  
David Brown ◽  
Barbara Alemar ◽  
...  
Keyword(s):  
Massively Parallel Sequencing ◽  
Massively Parallel ◽  
Sequencing Analysis ◽  
Parallel Sequencing ◽  
Melanocytic Naevi

scholarly journals Estimating included animal species in mixed crude drugs derived from animals using massively parallel sequencing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hiroaki Nakanishi ◽  
Katsumi Yoneyama ◽  
Masaaki Hara ◽  
Aya Takada ◽  
Kazuyuki Saito
Keyword(s):  
Animal Species ◽  
Massively Parallel Sequencing ◽  
Massively Parallel ◽  
12S Rrna ◽  
Sequencing Analysis ◽  
Cytochrome Oxidase Subunit ◽  
Parallel Sequencing ◽  
Mitochondrial 12S Rrna ◽  
Crude Drugs ◽  
Mixed Crude

AbstractWe developed a method that can detect each animal species of origin for crude drugs derived from multiple animal species based on massively parallel sequencing analysis of mitochondrial genes. The crude drugs derived from animals investigated in this study were Cervi Parvum Cornu and Trogopterorum feces, which are derived from a mix of different animal species, two chopped cicada sloughs, and two commercial Kampo drugs. The mitochondrial 12S rRNA, 16S rRNA, and cytochrome oxidase subunit I gene regions were amplified and sequenced using MiSeq. The ratios of haplotype to total number of sequences reads were calculated after sequence extraction and trimming. Haplotypes that exceeded the threshold were defined as positive haplotypes, which were compared with all available sequences using BLAST. In the Cervi Parvum Cornu and Trogopterorum feces samples, the haplotype ratios corresponded roughly to the mixture ratios, although there was a slight difference from mixture ratios depending on the gene examined. This method could also roughly estimate the compositions of chopped cicada sloughs and Kampo drugs. This analysis, whereby the sequences of several genes are elucidated, is better for identifying the included animal species. This method should be useful for quality control of crude drugs and Kampo drugs.

A microhaplotypes panel for massively parallel sequencing analysis of DNA mixtures

2019 ◽  
Vol 40 ◽  
pp. 140-149 ◽  
Author(s):  
Peng Chen ◽  
Chuwei Deng ◽  
Zheng Li ◽  
Yan Pu ◽  
Jiawen Yang ◽  
...  
Keyword(s):  
Massively Parallel Sequencing ◽  
Massively Parallel ◽  
Sequencing Analysis ◽  
Dna Mixtures ◽  
Parallel Sequencing
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