Serum Gas6 and Axl as non‐invasive biomarkers of advanced histological stage in primary biliary cholangitis

2020 ◽  
Vol 50 (12) ◽  
pp. 1337-1346
Author(s):  
Manabu Hayashi ◽  
Kazumichi Abe ◽  
Masashi Fujita ◽  
Atsushi Takahashi ◽  
Yuko Hashimoto ◽  
...  
Keyword(s):  
Primary Biliary Cholangitis ◽  
Non Invasive ◽  
Histological Stage

NON-INVASIVE DIAGNOSIS AND FOLLOW-UP OF PRIMARY BILIARY CHOLANGITIS

2021 ◽  
pp. 101770
Author(s):  
Christophe Corpechot ◽  
Alexandra Heurgue ◽  
Marie Decraecker ◽  
Florence Tanne ◽  
Pascal Potier ◽  
...  
Keyword(s):  
Primary Biliary Cholangitis ◽  
Non Invasive

Establishing Reliability Criteria for Liver ElastPQ Shear Wave Elastography (ElastPQ-SWE): Comparison Between 10, 5 and 3 Measurements

2019 ◽  
Author(s):  
Davide Roccarina ◽  
Laura Iogna Prat ◽  
Elena Buzzetti ◽  
Marta Guerrero Misas ◽  
Francesco Marcello Aricó ◽  
...  
Keyword(s):  
Liver Disease ◽  
Diagnostic Performance ◽  
Liver Stiffness ◽  
Quality Criterion ◽  
Quality Criteria ◽  
Shear Wave Elastography ◽  
Primary Biliary Cholangitis ◽  
Alcoholic Fatty Liver ◽  
Non Invasive ◽  
The Difference

Abstract Purpose ElastPQ is a new elastography technique for non-invasive liver fibrosis staging. However, it does not have validated reliability criteria. We tested the reliability of a different number of measurements in patients with chronic liver disease and explored whether the application of quality criteria improves the diagnostic performance. Materials and Methods All patients underwent liver stiffness assessment (LSM) with ElastPQ and Fibroscan (F-TE). The mean, median, standard deviation (SD) and interquartile range (IQR) of 10, 5 and 3 measurements were retrospectively collected for each patient and compared to each other. Liver histology was available in a subset of patients. Results Overall, 400 patients met the inclusion criteria. Non-alcoholic fatty liver disease (NAFLD) was the most represented etiology (75 %), followed by primary sclerosing cholangitis (PSC), primary biliary cholangitis (PBC) and autoimmune hepatitis (AIH). The correlation of medians was significantly better between 10 and 5 measurements than between 10 and 3. The difference of medians was significant only in the comparison between 10 and 3 measurements. The correlation between ElastPQ and F-TE was equally good for 10 and 5 measurements and significantly improved after an IQR/median ≤ 30 % was applied. The diagnostic performance of ElastPQ was better with the median value of 10 and 5 measurements and improved if LSM values were obtained with IQR/M ≤ 30 %. Conclusion The median value of 5 valid LSMs suffices for the reliable estimation of liver stiffness using ElastPQ. The quality criterion of IQR/M ≤ 30 % should also be followed when using this technique.

Mo1447 - Bile Duct Damage and Cirrhosis in Primary Biliary Cholangitis: An Investigation of Non-Invasive Biomarkers

2018 ◽  
Vol 154 (6) ◽  
pp. S-1209
Author(s):  
Natasha Kamal ◽  
Pallavi Surana ◽  
Mazen Noureddin ◽  
David Kleiner ◽  
Jay Hoofnagle ◽  
...  
Keyword(s):  
Bile Duct ◽  
Primary Biliary Cholangitis ◽  
Non Invasive ◽  
Bile Duct Damage

Minimal hepatic encephalopathy may be present despite the absence of non-invasive and elastography evidence of cirrhosis in patients with primary biliary cholangitis

2021 ◽  
Vol 66 (2) ◽  
pp. 293-301
Author(s):  
Dagmara Bogdanowska – Charkiewicz ◽  
Pawel Rogalski ◽  
Jacek Janica ◽  
Magdalena Rogalska – Plonska ◽  
Elzbieta Aleksiejuk ◽  
...  
Keyword(s):  
Hepatic Encephalopathy ◽  
Minimal Hepatic Encephalopathy ◽  
Primary Biliary Cholangitis ◽  
Non Invasive

Combination of transient elastography with serum-based non-invasive tests improves prediction of liver fibrosis in patients with chronic hepatitis C: a prospective cohort study

2015 ◽  
Vol 22 (2) ◽  
pp. 77-84
Author(s):  
Romanas Zykus ◽  
Laimas Jonaitis ◽  
Vitalija Petrenkienė ◽  
Inga Gudinavičienė ◽  
Limas Kupčinskas
Keyword(s):  
Liver Fibrosis ◽  
Transient Elastography ◽  
Curve Analysis ◽  
Roc Curve Analysis ◽  
Cutoff Value ◽  
Non Invasive ◽  
Metavir Score ◽  
Combined Use ◽  
Chronic Hcv ◽  
Histological Stage

The work was carried out at the Lithuanian University of Health Sciences Hospital Kaunas Clinics. Background. To date, there is not enough data to conclude whether the combination of different non-invasive liver fibrosis tests could improve the accuracy in prediction of liver fibrosis. The aim of this study was to assess correlation between transient elastography (TE), aspartate aminotransferase to platelet ratio index (APRI), fibrosis 4 score (FIB4) and histological stage of fibrosis (F). Materials and methods. In this prospective study the correlation of TE, APRI and FIB4 with the stage of fibrosis was assessed in 140 patients with chronic HCV hepatitis. TE, APRI and FIB4 were measured the same day before biopsy. Fibrosis was evaluated using the METAVIR score. Cut-off values were established by applying the ROC curve analysis. All non-invasive tests were combined into pairs in order to evaluate the accuracy of fibrosis prediction. Results. The stage of fibrosis correlated with TE (R-0.74), FIB4 (R-0.67) and APRI (R-0.58). To detect F4 TE cut-off value 12.1 kPa had 93.8% sensitivity and 85% specificity; APRI cut-off value 1.42 (84.4/81.1) and FIB4 cutoff value 2.89 (84.4/84.0) were established. To determine F ≥ 3 – 10.3 kPa (91.1/83.9), 1.28 (77.8/78.5), 2.28 (84.4/81.7); F ≥ 2 8.5 kPa (80.9/74.3), 1.12 (72.1/78.6), 1.63 (82.4/75.7); F  ≥  1 5.35  kPa (85.4/100), 0.45 (89.2/87.5), 0.89 (87.7/75). Significant increase of accuracy was observed in TE/APRI (p – 0.008) and FIB4/APRI (p – 0.02) groups to predict F ≥ 1, and TE/FIB4 to predict F ≥ 2 (p – 0.04) and F ≥ 1 (p – 0.04). Conclusions. Combined use of TE/APRI, FIB4/APRI increased the accuracy to predict F ≥ 1, and TE/FIB4 combination increased the accuracy to predict F ≥ 2 and F ≥ 1.

scholarly journals Evaluation of circulating cell-free DNA in cholestatic liver disease using liver-specific methylation markers

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sohan Punia ◽  
Brian D. Juran ◽  
Ahmad H. Ali ◽  
Erik M. Schlicht ◽  
Raymond M. Moore ◽  
...  
Keyword(s):  
Cell Death ◽  
Liver Disease ◽  
Liver Diseases ◽  
Methylation Status ◽  
Primary Biliary Cholangitis ◽  
Specific Cell ◽  
Cholestatic Liver Disease ◽  
Cell Free Dna ◽  
Non Invasive ◽  
Free Dna

Abstract Background Quantification of circulating organ-specific cell-free DNA (cfDNA) provides a sensitive measure of ongoing cell death that could benefit evaluation of the cholestatic liver diseases primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC), which lack reliable non-invasive biomarkers. Our goal in this pilot study was to determine whether liver-specific cfDNA levels are increased in PBC and PSC patients relative to controls and in advanced versus early disease, to evaluate their potential as novel disease biomarkers. Methods Peripheral blood derived bisulfite-treated DNA was PCR amplified from patients with PBC (n = 48), PSC (n = 48) and controls (n = 96) to evaluate methylation status at 16 CpG sites reported to be specifically unmethylated in liver tissue near the genes IGF2R, ITIH4 and VTN. Amplicons were used to prepare paired end libraries which were sequenced on a MiSeq sequencer. Trimmed reads were aligned and used to determine unmethylation ratios and to calculate concentration of liver-specific cfDNA. Comparisons between groups were performed using the two-tailed Mann–Whitney Test and relationships between variables were evaluated using Pearson’s Correlation. Results Levels of liver-specific cfDNA, as measured at the 3 genetic loci, were increased in PBC and PSC patients relative to controls and in late-stage relative to early-stage patients. As well, cfDNA levels were correlated with levels of alkaline phosphatase, a commonly used biochemical test to evaluate disease severity in liver disease, in patients, but not in controls. Conclusions cfDNA offers promise as a non-invasive liquid-biopsy to evaluate liver-specific cell-death in patients with cholestatic liver diseases.

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