Liver tissue metabolic profiling and pathways of non-alcoholic steatohepatitis in rats

2017 ◽  
Vol 47 (13) ◽  
pp. 1484-1493 ◽  
Author(s):  
Suwen Qi ◽  
Si Huang ◽  
Xin Chen ◽  
Qin Huo ◽  
Ni Xie ◽  
...  
Keyword(s):  
Metabolic Profiling ◽  
Liver Tissue ◽  
Alcoholic Steatohepatitis ◽  
Tissue Metabolic Profiling

scholarly journals Soybean oil attenuates the onset of non-alcoholic steatohepatitis and insulin resistance

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Victor Sanchez ◽  
Anna Janina Engstler ◽  
Annette Brandt ◽  
Cheng Jun Jin ◽  
Dragana Rajcic ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Soybean Oil ◽  
Mrna Expression ◽  
Liver Tissue ◽  
Metabolic Diseases ◽  
Insulin Receptors ◽  
Signaling Cascade ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Steatohepatitis ◽  
Tlr4 Mrna

AbstractBackground and Aims:General overnutrition, and a diet rich in fat and sugar are among the key risk factors for the development of metabolic diseases including diabetes type 2 and non-alcoholic fatty liver diseases (NAFLD). While being among the most commonly consumed oils world-wide, the effects of soybean oil on the development of metabolic diseases are yet not understood. Indeed, existing data is in part contradictory. Based on this background, the aim of the present study was to investigate the effects of soybean oil consumption on the development of non-alcoholic steatohepatitis (NASH), the more progressed stage of NAFLD, and insulin resistance.Methods:Female C57BL/6J mice were fed either a liquid standard control diet (C) or a liquid fat-, fructose- and cholesterol-rich diet (FFC, 25E% butterfat, 50% (wt/wt) fructose, 0.155% (wt/wt) cholesterol) or a FFC supplemented with soybean oil (FFC + S, 21E% butterfat + 4E% soy oil), with FFC and FFC + S-fed groups being pair fed for 13 weeks to induce steatohepatitis. After 7 and 12 weeks of feeding, a glucose tolerance test was performed. Indicators of liver damage, inflammation, intestinal barrier function and markers of insulin signaling cascade were measured in intestinal, liver and muscle tissue.Results:As expected FFC-fed mice developed early signs of NASH and insulin resistance. Despite similar caloric intake and body weight gain, development of NASH and insulin resistance were significantly attenuated in FFC + S-fed mice when compared to FFC-fed animals. Indeed, signs of hepatic inflammation e.g. number of inflammatory foci and neutrophils as well as activity of transaminase in plasma were almost at the level of control in FFC + S-fed mice. Prevalence of macrovesicular steatosis being the predominant type of fat accumulation found in FFC-fed animals was also markedly lower in FFC + S-fed mice. Furthermore, while in muscle and liver tissue of FFC-fed mice insulin receptors mRNA expression was significantly different from C-fed animals, insulin receptors expression in FFC + S-fed mice was almost at the level of controls. The protective effects of soybean oil supplementation on the development of metabolic alterations were associated with a protection against the induction of TLR4 mRNA expression and dependent signaling molecules in liver tissue.Conclusion:Taking together, our results indicate that supplementation with soybean oil may attenuate the development of diet-induced NASH and insulin resistance in mice and that this may be related to alteration of TLR4-dependent signaling cascade in liver tissue. (Funded in parts by UFOP e.V.)

Phenotyping murine models of non-alcoholic fatty liver disease through metabolic profiling of intact liver tissue

2009 ◽  
Vol 116 (5) ◽  
pp. 403-413 ◽  
Author(s):  
Jeremy F. L. Cobbold ◽  
Quentin M. Anstee ◽  
Robert D. Goldin ◽  
Horace R. T. Williams ◽  
Helen C. Matthews ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Metabolic Profiling ◽  
Liver Tissue ◽  
Fatty Liver Disease ◽  
Magic Angle ◽  
Murine Models ◽  
Alcoholic Fatty Liver ◽  
The Metabolic Syndrome ◽  
Alcoholic Fatty Liver Disease

NAFLD (non-alcoholic fatty liver disease) is a common cause of chronic liver disease associated with the metabolic syndrome. Effective techniques are needed to investigate the potential of animal models of NAFLD. The present study aimed to characterize murine models of NAFLD by metabolic profiling of intact liver tissue. Mice of three strains (BALB/c, C3H and the novel mutant, Gena/263) were fed a control or high-fat diet. Biometric, biochemical and histological analysis demonstrated a spectrum of NAFLD from normal liver to steatohepatitis. Metabolic profiling of intact liver tissue, using 1H MAS (proton magic angle spinning) MRS (magnetic resonance spectroscopy), showed an increase in the total lipid-to-water ratio, a decrease in polyunsaturation indices and a decrease in total choline with increasing disease severity. Principal components analysis and partial least-squares discriminant analysis showed separation of each model from its control and of each model from the total dataset. Class membership from the whole dataset was predicted with 100% accuracy in six out of eight models. Those models with steatosis discriminated from those with steatohepatitis with 100% accuracy. The separation of histologically defined steatohepatitis from simple steatosis is clinically important. Indices derived from 1H MAS MRS studies may inform subsequent in vivo MRS studies at lower field strengths.

scholarly journals Tissue metabolic profiling of lymph node metastasis of colorectal cancer assessed by 1H NMR

2016 ◽  
Vol 36 (6) ◽  
pp. 3436-3448 ◽  
Author(s):  
Hailong Zhang ◽  
Liang Qiao ◽  
Xiaopeng Li ◽  
Yang Wan ◽  
Li Yang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
1H Nmr ◽  
Metabolic Profiling ◽  
Node Metastasis ◽  
Tissue Metabolic Profiling

Tissue metabolic profiling reveals major metabolic alteration in colorectal cancer

2021 ◽  
Author(s):  
Yao Shen ◽  
Mao Sun ◽  
Jun Zhu ◽  
Mengying Wei ◽  
Huichen Li ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mass Spectrometry ◽  
Metabolic Profiling ◽  
Tissue Samples ◽  
Metabolic Alteration ◽  
Tissue Metabolic Profiling

Novel insights into metabolism profiling of colorectal cancer by mass spectrometry-based metabolomics conducted on tissue samples.

Sign in / Sign up

Export Citation Format

Share Document