Ultra-deep sequencing analysis of resistance-associated variants during retreatment with simeprevir-based triple therapy after failure of telaprevir-based triple therapy in patients with genotype 1 hepatitis C virus infection

2016 ◽  
Vol 47 (8) ◽  
pp. 773-782 ◽  
Author(s):  
Naoki Morishita ◽  
Naoki Hiramatsu ◽  
Tsugiko Oze ◽  
Ayako Urabe ◽  
Yuki Tahata ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Triple Therapy ◽  
Deep Sequencing ◽  
Sequencing Analysis ◽  
Hepatitis C Virus Infection ◽  
Genotype 1 ◽  
Deep Sequencing Analysis

scholarly journals The reduction of miR146b-5p in monocytes and T cells could contribute to the immunopathogenesis of hepatitis C virus infection

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yasuteru Kondo ◽  
Takayuki Kogure ◽  
Masashi Ninomiya ◽  
Ryo Fukuda ◽  
Norikazu Monma ◽  
...  
Keyword(s):  
T Cells ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Clinical Samples ◽  
Sequencing Analysis ◽  
Hepatitis C Virus Infection ◽  
Deep Sequencing Analysis ◽  
Deep Sequence Analysis

Abstract It has been reported that various kinds of miRNAs could affect the pathogenesis of hepatitis C virus infection. Recently, our group reported that deep-sequencing analysis was useful to detect disease-specific miRNAs. The aim of this study is to identify the HCV-specific miRNAs that could contribute to the immunopathogenesis of HCV by using clinical samples and in vitro analysis. Five miRNAs (hsa-miR181a-2-3p, hsa-miR-374a-3p, hsa-miR374a-5p, hsa-miR-204-5p and hsa-miR146b-5p) were shown to be significantly downregulated in CH-C by deep sequence analysis. The average ratio (PBMCs miRNAs/serum miRNAs) of hsa-miR146b-5p was highest among all the miRNAs. Moreover, serum hsa-miR146b-5p was significantly down-regulated in CH-C patients in comparison to CH-B patients and healthy subjects. The expression of hsa-miR146b-5p in CD3+ T cells and CD14+ monocytes of CH-C patients was significantly lower than that of the other groups. The hsa-miR146b-5p expression in CD14+ monocytes of SVR patients treated with Peg-IFN/RBV was significantly higher than in those of non-SVR patients treated with Peg IFN/RBV. However, the hsa-miR146b-5p expression in CD14+ monocytes of SVR patients treated with DCV and ASV was comparable to that in monocytes of non-SVR patients treated with DCV and ASV. Moreover, the expression levels of hsa-miR146b-5p in CD14+ monocytes were significantly increased after achieving SVR and 1(OH)Vitamin D3 treatment. Further, the expression of HCV-Core could suppress miR146b-5p expression in immune cells and affect the expression of various kinds of cytokines by affecting the NF-κB signaling. In conclusion, the reduction of miR146b-5p in monocytes and T cells could contribute to the immunopathogenesis of hepatitis C virus infection.

Safety of Glecaprevir/Pibrentasvir in Adults with Chronic Genotype 1-6 Hepatitis C Virus Infection: An Integrated Analysis

2017 ◽  
Vol 152 (5) ◽  
pp. S1089 ◽  
Author(s):  
Jean-Francois DuFour ◽  
Eli Zuckerman ◽  
Neddie Zadeikis ◽  
Christophe Hezode ◽  
Seung Woon Paik ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Integrated Analysis ◽  
Hepatitis C Virus Infection ◽  
Genotype 1

scholarly journals Effectiveness of Telaprevir and Boceprevir Triple Therapy for Patients with Hepatitis C Virus Infection in a Large Integrated Care Setting

2014 ◽  
Vol 59 (12) ◽  
pp. 3043-3052 ◽  
Author(s):  
Jennifer C. Price ◽  
Rosemary C. Murphy ◽  
Valentina A. Shvachko ◽  
Mary Pat Pauly ◽  
M. Michele Manos
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Integrated Care ◽  
Triple Therapy ◽  
Care Setting ◽  
Hepatitis C Virus Infection

Sofosbuvir Plus Velpatasvir Combination Therapy for Treatment-Experienced Patients With Genotype 1 or 3 Hepatitis C Virus Infection

2015 ◽  
Vol 163 (11) ◽  
pp. 809 ◽  
Author(s):  
Stephen Pianko ◽  
Steven L. Flamm ◽  
Mitchell L. Shiffman ◽  
Sonal Kumar ◽  
Simone I. Strasser ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Combination Therapy ◽  
Hepatitis C Virus Infection ◽  
Genotype 1
Sign in / Sign up

Export Citation Format

Share Document