Helicobacter pylori induced reactive oxygen Species: A new and developing platform for detection

Helicobacter ◽  
2021 ◽  
Author(s):  
Utkarsh Jain ◽  
Kirti Saxena ◽  
Nidhi Chauhan
Keyword(s):  
Reactive Oxygen Species ◽  
Helicobacter Pylori ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Developing Platform

scholarly journals The Helicobacter pylori CZB Cytoplasmic Chemoreceptor TlpD Forms an Autonomous Polar Chemotaxis Signaling Complex That Mediates a Tactic Response to Oxidative Stress

2016 ◽  
Vol 198 (11) ◽  
pp. 1563-1575 ◽  
Author(s):  
Kieran D. Collins ◽  
Tessa M. Andermann ◽  
Jenny Draper ◽  
Lisa Sanders ◽  
Susan M. Williams ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Helicobacter Pylori ◽  
Molecular Mechanisms ◽  
Reactive Oxygen ◽  
Host Cells ◽  
Oxygen Species ◽  
Content Type ◽  
Signaling Complex ◽  
H Pylori

ABSTRACTCytoplasmic chemoreceptors are widespread among prokaryotes but are far less understood than transmembrane chemoreceptors, despite being implicated in many processes. One such cytoplasmic chemoreceptor isHelicobacter pyloriTlpD, which is required for stomach colonization and drives a chemotaxis response to cellular energy levels. Neither the signals sensed by TlpD nor its molecular mechanisms of action are known. We report here that TlpD functions independently of the other chemoreceptors. When TlpD is the sole chemoreceptor, it is able to localize to the pole and recruits CheW, CheA, and at least two CheV proteins to this location. It loses the normal membrane association that appears to be driven by interactions with other chemoreceptors and with CheW, CheV1, and CheA. These results suggest that TlpD can form an autonomous signaling unit. We further determined that TlpD mediates a repellent chemotaxis response to conditions that promote oxidative stress, including being in the presence of iron, hydrogen peroxide, paraquat, and metronidazole. Last, we found that all testedH. pyloristrains express TlpD, whereas other chemoreceptors were present to various degrees. Our data suggest a model in which TlpD coordinates a signaling complex that responds to oxidative stress and may allowH. pylorito avoid areas of the stomach with high concentrations of reactive oxygen species.IMPORTANCEHelicobacter pylorisenses its environment with proteins called chemoreceptors. Chemoreceptors integrate this sensory information to affect flagellum-based motility in a process called chemotaxis. Chemotaxis is employed during infection and presumably aidsH. pyloriin encountering and colonizing preferred niches. A cytoplasmic chemoreceptor named TlpD is particularly important in this process, and we report here that this chemoreceptor is able to operate independently of other chemoreceptors to organize a chemotaxis signaling complex and mediate a repellent response to oxidative stress conditions.H. pyloriencounters and must cope with oxidative stress during infection due to oxygen and reactive oxygen species produced by host cells. TlpD's repellent response may allow the bacteria to escape niches experiencing inflammation and elevated reactive oxygen species (ROS) production.

Production of Chemokines and Reactive Oxygen Species by Human Neutrophils Stimulated by Helicobacter pylori

Helicobacter ◽  
2002 ◽  
Vol 7 (3) ◽  
pp. 170-174 ◽  
Author(s):  
Tadashi Shimoyama ◽  
Shinsaku Fukuda ◽  
Qiang Liu ◽  
Shigeyuki Nakaji ◽  
Yoshihiro Fukuda ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Helicobacter Pylori ◽  
Reactive Oxygen ◽  
Human Neutrophils ◽  
Oxygen Species

Impact of reactive oxygen species generation on Helicobacter pylori-related extragastric diseases: a hypothesis

2017 ◽  
Vol 51 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Jannis Kountouras ◽  
Marina Boziki ◽  
Stergios A. Polyzos ◽  
Panagiotis Katsinelos ◽  
Emmanouel Gavalas ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Helicobacter Pylori ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Oxygen Species
Sign in / Sign up

Export Citation Format

Share Document