Detection ofHelicobacter pyloriin stool samples of young children using real-time polymerase chain reaction

Helicobacter ◽  
2017 ◽  
Vol 23 (1) ◽  
pp. e12450 ◽  
Author(s):  
Gany Beer-Davidson ◽  
Musa Hindiyeh ◽  
Khitam Muhsen
Keyword(s):  
Polymerase Chain Reaction ◽  
Young Children ◽  
Real Time ◽  
Chain Reaction ◽  
Stool Samples ◽  
Polymerase Chain

Real-time polymerase chain reaction for detection of Isospora belli in stool samples

2008 ◽  
Vol 61 (3) ◽  
pp. 280-283 ◽  
Author(s):  
Robert-Jan ten Hove ◽  
Lisette van Lieshout ◽  
Eric A.T. Brienen ◽  
M. Arantza Perez ◽  
Jaco J. Verweij
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chain Reaction ◽  
Stool Samples ◽  
Polymerase Chain

scholarly journals Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil

2012 ◽  
Vol 107 (4) ◽  
pp. 476-479 ◽  
Author(s):  
Roberta Flávia Ribeiro Rolando ◽  
Sidnei da Silva ◽  
Regina Helena Saramago Peralta ◽  
Alexandre Januário da Silva ◽  
Flavia de Souza Cunha ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Rio De Janeiro ◽  
Chain Reaction ◽  
Stool Samples ◽  
Polymerase Chain

scholarly journals Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Kaewkanya Nakjarung ◽  
Ladaporn Bodhidatta ◽  
Pimmnapar Neesanant ◽  
Paphavee Lertsethtakarn ◽  
Orntipa Sethabutr ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Young Children ◽  
Real Time ◽  
Pediatric Hospital ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Phnom Penh ◽  
Stool Samples ◽  
Polymerase Chain ◽  
Predominant Strain

This study investigated the genetic diversity of noroviruses identified from a previous surveillance study conducted at the National Pediatric Hospital in Phnom Penh, Cambodia, from 2004 to 2006. In the previous study, 926 stool samples were collected from children aged 3–60 months with acute diarrhea (cases) and without diarrhea (controls) with reported 6.7% of cases and 3.2% of controls being positive for norovirus. The initial norovirus diagnostic assay was performed with real-time reverse transcription-polymerase chain reaction (real-time RT PCR) which also distinguished between genogroups I and II (GI and GII). Norovirus infection was most commonly detected in children aged 12–23 months in both cases and controls. Norovirus Genotyping Tool and phylogenetic analysis of partial sequences of the 3′ end of the RNA-dependent RNA Polymerase (RdRp) and the capsid domain region were employed to assign genotypes of the norovirus strains. GII.4 was the most predominant capsid genotype detected at 39.5% followed by GII.6 at 14.9%. The GII.4 Hunter 2004 variant was the predominant strain detected. Six RdRP/capsid recombinants including GII.P7/GII.6, GII.P7/GII.14, GII.P7/GII.20, GII.P12/GII.13, GII.P17/GII.16, and GII.P21/GII.3 were also identified. This study of norovirus infection in young children in Cambodia suggests genetic diversity of norovirus as reported worldwide.

scholarly journals Evaluation of immunochromatographic tests for the rapid detection of the emerging GII.17 norovirus in stool samples, January 2016

2016 ◽  
Vol 21 (4) ◽  
Author(s):  
Lucie Théry ◽  
Maxime Bidalot ◽  
Pierre Pothier ◽  
Katia Ambert-Balay
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Virus Titre ◽  
Chain Reaction ◽  
Diagnostic Assays ◽  
Stool Samples ◽  
Polymerase Chain

A novel GII.17 norovirus emerged in Asia in the winter of 2014/15. A worldwide spread is conceivable and norovirus diagnostic assays need to be evaluated to investigate if they adequately detect this emerging genotype. Seven immunochromatographic kits commercially available in Europe were evaluated on ten stool samples where GII.17 virus had been quantified by real-time reverse transcription-polymerase chain reaction. All the kits detected GII.17 with various sensitivities, partly depending on the virus titre.

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