scholarly journals ZomB is essential for chemotaxis of Vibrio alginolyticus by the rotational direction control of the polar flagellar motor

2021 ◽  
Author(s):  
Norihiro Takekawa ◽  
Tatsuro Nishikino ◽  
Kiyoshiro Hori ◽  
Seiji Kojima ◽  
Katsumi Imada ◽  
...  
Keyword(s):  
Vibrio Alginolyticus ◽  
Flagellar Motor ◽  
Direction Control ◽  
Rotational Direction

scholarly journals ZomB is essential for chemotaxis of Vibrio alginolyticus by the rotational direction control of the polar flagellar motor

2021 ◽  
Author(s):  
Norihiro Takekawa ◽  
Tatsuro Nishikino ◽  
Kiyoshiro Hori ◽  
Seiji Kojima ◽  
Michio Homma
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Transmembrane Protein ◽  
Shewanella Putrefaciens ◽  
Vibrio Alginolyticus ◽  
Flagellar Motor ◽  
Signaling Protein ◽  
Direction Control ◽  
Flagellar Rotation ◽  
Rotational Direction

Bacteria exhibit chemotaxis by controlling flagellar rotation to move toward preferred places or away from non-preferred places. The change in rotation is triggered by the binding of the chemotaxis signaling protein CheY to the C-ring in the flagellar motor. Some specific bacteria, including Vibrio spp. and Shewanella spp. have a single transmembrane protein called ZomB. ZomB is essential for controlling the flagellar rotational direction in Shewanella putrefaciens and Vibrio parahaemolyticus. In this study, we confirmed that the zomB deletion results only in the counterclockwise (CCW) rotation of the motor in Vibrio alginolyticus as previously reported in other bacteria. We found that ZomB is not required for the clockwise (CW) rotation-fixing phenotype caused by mutations in fliG and fliM, and that ZomB is essential for CW rotation induced by overproduction of CheY. Purified ZomB proteins form multimers, indicating that ZomB functions as a complex. ZomB may interact with a protein involved in the flagellar rotation, stator proteins or rotor proteins. We found that ZomB is a new player in chemotaxis and is required for the rotational control in addition to CheY in Vibrio alginolyticus.

scholarly journals The flagellar motor of Vibrio alginolyticus undergoes major structural remodeling during rotational switching

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Brittany L Carroll ◽  
Tatsuro Nishikino ◽  
Wangbiao Guo ◽  
Shiwei Zhu ◽  
Seiji Kojima ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Electron Tomography ◽  
Protein Composition ◽  
Vibrio Alginolyticus ◽  
Flagellar Motor ◽  
Conformational Rearrangement ◽  
Structural Remodeling ◽  
Cryo Electron Tomography ◽  
Bacterial Flagellar Motor ◽  
Rotational Direction

The bacterial flagellar motor switches rotational direction between counterclockwise (CCW) and clockwise (CW) to direct the migration of the cell. The cytoplasmic ring (C-ring) of the motor, which is composed of FliG, FliM, and FliN, is known for controlling the rotational sense of the flagellum. However, the mechanism underlying rotational switching remains elusive. Here, we deployed cryo-electron tomography to visualize the C-ring in two rotational biased mutants in Vibrio alginolyticus. We determined the C-ring molecular architectures, providing novel insights into the mechanism of rotational switching. We report that the C-ring maintained 34-fold symmetry in both rotational senses, and the protein composition remained constant. The two structures show FliG conformational changes elicit a large conformational rearrangement of the rotor complex that coincides with rotational switching of the flagellum. FliM and FliN form a stable spiral-shaped base of the C-ring, likely stabilizing the C-ring during the conformational remodeling.

scholarly journals 3P177 Pressure-velocity relationship of sodium-driven flagellar motor of Vibrio alginolyticus(Molecular motors,Oral Presentations)

2007 ◽  
Vol 47 (supplement) ◽  
pp. S247
Author(s):  
Yoshiki Shimoda ◽  
Masayoshi Nishiyama ◽  
Seiji Kojima ◽  
Michio Homma ◽  
Yoshihumi Kimura ◽  
...  
Keyword(s):  
Molecular Motors ◽  
Vibrio Alginolyticus ◽  
Flagellar Motor ◽  
Velocity Relationship ◽  
Relationship Of ◽  
Oral Presentations

Torque–speed Relationship of the Na+-driven Flagellar Motor of Vibrio alginolyticus

2003 ◽  
Vol 327 (5) ◽  
pp. 1043-1051 ◽  
Author(s):  
Yoshiyuki Sowa ◽  
Hiroyuki Hotta ◽  
Michio Homma ◽  
Akihiko Ishijima
Keyword(s):  
Vibrio Alginolyticus ◽  
Flagellar Motor ◽  
Relationship Of

Intelligent rotational direction control of passive robotic joint with embedded sensors

2013 ◽  
Vol 40 (4) ◽  
pp. 1265-1273 ◽  
Author(s):  
Dalibor Petković ◽  
Mirna Issa ◽  
Nenad D. Pavlović ◽  
Lena Zentner
Keyword(s):  
Embedded Sensors ◽  
Direction Control ◽  
Rotational Direction
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