Discovery of an NRF1-specific inducer from a large-scale chemical library using a direct NRF1-protein monitoring system

2015 ◽  
Vol 20 (7) ◽  
pp. 563-577 ◽  
Author(s):  
Tadayuki Tsujita ◽  
Liam Baird ◽  
Yuki Furusawa ◽  
Fumiki Katsuoka ◽  
Yoshika Hou ◽  
...  
Keyword(s):  
Monitoring System ◽  
Large Scale ◽  
Chemical Library

scholarly journals Identification of a juvenile-hormone signaling inhibitor via high-throughput screening of a chemical library

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Takumi Kayukawa ◽  
Kenjiro Furuta ◽  
Keisuke Nagamine ◽  
Tetsuro Shinoda ◽  
Kiyoaki Yonesu ◽  
...  
Keyword(s):  
Juvenile Hormone ◽  
Cell Line ◽  
Signaling Pathway ◽  
High Throughput ◽  
High Throughput Screening ◽  
Large Scale ◽  
Ex Vivo ◽  
Agricultural Field ◽  
Chemical Library ◽  
Field Development

Abstract Insecticide resistance has recently become a serious problem in the agricultural field. Development of insecticides with new mechanisms of action is essential to overcome this limitation. Juvenile hormone (JH) is an insect-specific hormone that plays key roles in maintaining the larval stage of insects. Hence, JH signaling pathway is considered a suitable target in the development of novel insecticides; however, only a few JH signaling inhibitors (JHSIs) have been reported, and no practical JHSIs have been developed. Here, we established a high-throughput screening (HTS) system for exploration of novel JHSIs using a Bombyx mori cell line (BmN_JF&AR cells) and carried out a large-scale screening in this cell line using a chemical library. The four-step HTS yielded 69 compounds as candidate JHSIs. Topical application of JHSI48 to B. mori larvae caused precocious metamorphosis. In ex vivo culture of the epidermis, JHSI48 suppressed the expression of the Krüppel homolog 1 gene, which is directly activated by JH-liganded receptor. Moreover, JHSI48 caused a parallel rightward shift in the JH response curve, suggesting that JHSI48 possesses a competitive antagonist-like activity. Thus, large-scale HTS using chemical libraries may have applications in development of future insecticides targeting the JH signaling pathway.

scholarly journals Service-driven inter-domain QoS monitoring system for large-scale IP and DVB networks

2006 ◽  
Vol 29 (10) ◽  
pp. 1687-1695 ◽  
Author(s):  
A. Mehaoua ◽  
T. Ahmed ◽  
H. Asgari ◽  
M. Sidibe ◽  
A. Nafaa ◽  
...  
Keyword(s):  
Monitoring System ◽  
Large Scale ◽  
Qos Monitoring

Mobile Agent-Based Computing Resource and Usage Monitoring at Large Scale Computer Centers

2011 ◽  
Author(s):  
Zhixin Tie ◽  
David Ko ◽  
Harry H. Cheng
Keyword(s):  
Monitoring System ◽  
Mobile Agent ◽  
Computer Games ◽  
Data Exchange ◽  
Large Scale ◽  
Agent Based ◽  
Computer Center ◽  
Control Command ◽  
Computer Centers ◽  
Computer Resources

Mobile agent technology has become an important approach for the design and development of distributed systems. However, there is little research regarding the monitoring of computer resources and usage at large scale distributed computer centers. This paper presents a mobile agent-based system called the Mobile Agent Based Computer Monitoring System (MABCMS) that supports the dynamic sending and executing of control command, dynamic data exchange, and dynamic deployment of mobile code in C/C++. Based on the Mobile-C library, agents can call low level functions in binary dynamic or static libraries, and thus can monitor computer resources and usage conveniently and efficiently. Two experimental applications have been designed using the MABCMS. The experiments were conducted in a university computer center with hundreds of computer workstations and 15 server machines. The first experiment uses the MABCMS to detect improper usage of the computer workstations, such as playing computer games. The second experimental application uses the MABCMS to detect system resources such as available hard disk space. The experiments show that the mobile agent based monitoring system is an effective method for detecting and interacting with students playing computer games and a practical way to monitor computer resources in large scale distributed computer centers.

Monitoring of a Grid Storage Virtualization Service

2013 ◽  
Vol 5 (1) ◽  
pp. 53-69
Author(s):  
Jacques Jorda ◽  
Aurélien Ortiz ◽  
Abdelaziz M’zoughi ◽  
Salam Traboulsi
Keyword(s):  
Monitoring System ◽  
Data Storage ◽  
Large Scale ◽  
Distributed Storage ◽  
Storage System ◽  
Data Access ◽  
Data Placement ◽  
Workload Prediction ◽  
Storage Virtualization

Grid computing is commonly used for large scale application requiring huge computation capabilities. In such distributed architectures, the data storage on the distributed storage resources must be handled by a dedicated storage system to ensure the required quality of service. In order to simplify the data placement on nodes and to increase the performance of applications, a storage virtualization layer can be used. This layer can be a single parallel filesystem (like GPFS) or a more complex middleware. The latter is preferred as it allows the data placement on the nodes to be tuned to increase both the reliability and the performance of data access. Thus, in such a middleware, a dedicated monitoring system must be used to ensure optimal performance. In this paper, the authors briefly introduce the Visage middleware – a middleware for storage virtualization. They present the most broadly used grid monitoring systems, and explain why they are not adequate for virtualized storage monitoring. The authors then present the architecture of their monitoring system dedicated to storage virtualization. We introduce the workload prediction model used to define the best node for data placement, and show on a simple experiment its accuracy.

scholarly journals High-Content Phenotypic Profiling in Esophageal Adenocarcinoma Identifies Selectively Active Pharmacological Classes of Drugs for Repurposing and Chemical Starting Points for Novel Drug Discovery

2020 ◽  
Vol 25 (7) ◽  
pp. 770-782 ◽  
Author(s):  
Rebecca E. Hughes ◽  
Richard J. R. Elliott ◽  
Alison F. Munro ◽  
Ashraff Makda ◽  
J. Robert O’Neill ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Esophageal Adenocarcinoma ◽  
Cell Lines ◽  
Cancer Biology ◽  
Large Scale ◽  
Chemical Library ◽  
Screening Platform ◽  
Poor Outcomes ◽  
Cell Phenotypes ◽  
Selective Activity

Esophageal adenocarcinoma (EAC) is a highly heterogeneous disease, dominated by large-scale genomic rearrangements and copy number alterations. Such characteristics have hampered conventional target-directed drug discovery and personalized medicine strategies, contributing to poor outcomes for patients. We describe the application of a high-content Cell Painting assay to profile the phenotypic response of 19,555 compounds across a panel of six EAC cell lines and two tissue-matched control lines. We built an automated high-content image analysis pipeline to identify compounds that selectively modified the phenotype of EAC cell lines. We further trained a machine-learning model to predict the mechanism of action of EAC selective compounds using phenotypic fingerprints from a library of reference compounds. We identified a number of phenotypic clusters enriched with similar pharmacological classes, including methotrexate and three other antimetabolites that are highly selective for EAC cell lines. We further identify a small number of hits from our diverse chemical library that show potent and selective activity for EAC cell lines and that do not cluster with the reference library of compounds, indicating they may be selectively targeting novel esophageal cancer biology. Overall, our results demonstrate that our EAC phenotypic screening platform can identify existing pharmacologic classes and novel compounds with selective activity for EAC cell phenotypes.

A large-scale customer-accessible energy monitoring system

2016 ◽  
Author(s):  
Rafael Nilson Rodrigues ◽  
Juliano Kasmirski Zatta ◽  
Jonas Vieira de Souza ◽  
Anna Luiza Espindola ◽  
Eduardo Galera de Carvalho
Keyword(s):  
Monitoring System ◽  
Large Scale ◽  
Energy Monitoring
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