Effects of adding acetic acid and molasses on fermentation quality and aerobic stability of total mixed ration silage prepared with hulless barley straw in Tibet

2014 ◽  
Vol 60 (4) ◽  
pp. 206-213 ◽  
Author(s):  
Xiaoyan Qiu ◽  
Gang Guo ◽  
Xianjun Yuan ◽  
Tao Shao
Keyword(s):  
Acetic Acid ◽  
Barley Straw ◽  
Hulless Barley ◽  
Total Mixed Ration ◽  
Aerobic Stability ◽  
Fermentation Quality

scholarly journals Evaluating fermentation quality, in vitro digestibility and aerobic stability of a total mixed ration ensiled with different additives on Tibet plateau

2021 ◽  
Vol 34 (2) ◽  
pp. 223-232
Author(s):  
Zhihao Dong ◽  
Siran Wang ◽  
Jie Zhao ◽  
Junfeng Li ◽  
Qinhua Liu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Tibetan Plateau ◽  
Propionic Acid ◽  
Utilization Efficiency ◽  
Tibet Plateau ◽  
In Vitro Digestibility ◽  
Total Mixed Ration ◽  
Aerobic Stability ◽  
Fermentation Quality

Objective: To investigate the improvement in utilization efficiency of total mixed ration (TMR) on Tibetan plateau, TMR were ensiled with different additives.Methods: A total of 150 experimental silos were prepared in a completely randomized design to evaluate the six treatments: i) control (without additive), ii) <i>Lactobacillus buchneri</i> (<i>L. buchneri</i>), iii) acetic acid, iv) propionic acid, v) 1,2-propanediol; and vi) 1-propanol. After 90 days of ensiling, silos were opened for fermentation quality and <i>in vitro</i> analysis, and then subjected to an aerobic stability test for 14 days.Results: Treating with <i>L. buchneri</i>, acetic acid, 1,2-propanediol and 1-propanol decreased propionic acid contents and yeast number, whereas increased (p<0.05) pH, acetic acid and ethanol contents in the fermented TMR. Despite increased dry matter (DM) loss in the TMRs treated with 1,2-propanediol and 1-pronanol, additives did not affect (p>0.05) all <i>in vitro</i> parameters including gas production at 24 h (GP<sub>24</sub>), GP rate constant, potential GP, <i>in vitro</i> DM digestibility and <i>in vitro</i> neutral detergent fibre digestibility. All additives improved the aerobic stability of ensiled TMR to different extents. Specially, aerobic stability of the ensiled TMR were substantially improved by <i>L. buchneri</i>, acetic acid, 1,2-propanediol, and 1-propanol, indicated by stable pH and lactic acid content during the aerobic stability test.Conclusion: <i>L. buchneri</i>, acetic acid, 1,2-propanediol, and 1-propanol had no adverse effect on <i>in vitro</i> digestibility, while ensiling TMR with the additives produced more acetic acid and ethanol, subsequently resulting in improvement of aerobic stability. There is a potential for some fermentation boosting additives to enhance aerobic stability of fermented TMR on Tibetan plateau.

scholarly journals Silage Fermentation, Bacterial Community, and Aerobic Stability of Total Mixed Ration Containing Wet Corn Gluten Feed and Corn Stover Prepared with Different Additives

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1775
Author(s):  
Guangning Zhang ◽  
Xinpeng Fang ◽  
Guanzhi Feng ◽  
Yang Li ◽  
Yonggen Zhang
Keyword(s):  
Lactic Acid ◽  
Bacterial Community ◽  
Corn Stover ◽  
Total Mixed Ration ◽  
Aerobic Stability ◽  
Corn Gluten ◽  
Corn Gluten Feed ◽  
Fermentation Quality ◽  
Wet Corn Gluten Feed ◽  
Gluten Feed

The objective of this study was to investigate the effects of different additives on the fermentation quality, bacterial community, and aerobic stability of total mixed ration (TMR) silage containing wet corn gluten feed (WCGF) and corn stover. The TMR was ensiled with four treatments: (1) no additive (control); (2) lactic acid bacteria (LAB); (3) fibrolytic enzyme (EN); (4) LAB + EN. The EN and LAB + EN decreased the neutral detergent fiber and acid detergent fiber contents. Additives led to a higher lactic acid (LA) content (p < 0.0001) compared to control at all ensiling times. Silages inoculated with LAB and LAB + EN had higher dry matter (p = 0.0007), LA (p < 0.0001) and acetic acid (AA) contents (p < 0.0001) compared to control. The LAB and LAB + EN had significantly lowest ammonia nitrogen among the treatments, while no significant difference occurred after days 7 of ensiling. Silages treated with LAB and LAB + EN had a higher LAB count (p < 0.0001) and a lower pH, yeast, and mold counts compared to other silages. The LAB and LAB + EN greatly increased the portions of Firmicutes and Lactobacillus (p < 0.0001, and p < 0.0001, respectively) and reduced undesirable bacteria. Inoculation with LAB + EN and LAB improved aerobic stability of TMR silages indicated by higher and more stable LA and AA contents, smaller rise in pH, and yeast count than other silages. The LAB + EN and LAB reduced microbial diversity and improved the fermentation quality and aerobic stability of TMR silage containing WCGF and corn stover.

Fermentation quality, in vitro digestibility and aerobic stability of total mixed ration silages prepared with whole-plant corn (Zea mays L.) and hulless barley (Hordeum vulgare L.) straw

2018 ◽  
Vol 58 (10) ◽  
pp. 1860 ◽  
Author(s):  
XianJun Yuan ◽  
AiYou Wen ◽  
Jian Wang ◽  
JunFeng Li ◽  
Seare T. Desta ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Ammonia Nitrogen ◽  
Stability Test ◽  
Small Scale ◽  
In Vitro Digestibility ◽  
Total Mixed Ration ◽  
Aerobic Stability ◽  
Fermentation Quality

This study was carried out to assess the effects of adding Lactobacillus plantarum, molasses or/and ethanol on the fermentation quality, in vitro digestibility and aerobic stability of total mixed ration (TMR) silage, which is well accepted in small-scale dairy farms in Tibet. Total mixed ration were ensiled in laboratory silos (1 L) and treated with (1) no additive (Control), (2) ethanol (E, 25 ml/kg fresh weight (FW)), (3) molasses (M, 30 g/kg FW); (4) Lactobacillus plantarum (L, 106cfu/g FW); (5) ethanol + molasses (EM); and (6) ethanol + Lactobacillus plantarum (EL). After 45 days of ensiling, six silos per treatment were opened for the fermentation quality and in vitro digestibility analyses, whereas 18 silos were used for the aerobic stability test for the following 9 days. All TMR silages were well preserved with dominant lactic acid (LA), low pH and ammonia nitrogen, and negligible propionic and butyric acid. The L and EL silages had the lowest pH and highest LA concentrations. The addition of ethanol did not inhibit silage fermentation as there were no significant differences for the pH, LA, acetic acid, negligible propionic acid or ammonia nitrogen content, lactic acid bacteria and yeast counts between Control and the E silage. During the aerobic stability test, pH increased by 1.39, 1.67, 1.69 and 0.74 for the Control, M, L and EM silages, but only 0.40 and 0.34 for E and EL silages, respectively. Upon exposure to air, the LA concentration in the L silage was evidently (P < 0.05) decreased, whereas LA concentration in the EL silage remained the highest value after the third day of aerobic exposure. Mean populations of aerobic bacteria and yeast in the E and EL silages were lower (P < 0.05) than those of the Control. These findings suggested that L. plantarum is effective in improving fermentation quality of TMR silages. Although the addition of ethanol in our study did not depress the fermentation of the TMR silages, it showed potential to inhibit the aerobic spoilage of TMR silages, either alone or in combination with the L. plantarum. It is concluded that L. plantarum combined with ethanol not only ensures better fermentation but also could improve aerobic stability.

scholarly journals The effect silage aditives supplementation on dynamic fermentation process, quality and aerobic stability of corn silage

2008 ◽  
Vol 56 (1) ◽  
pp. 157-164
Author(s):  
Václav Pyrochta ◽  
Libor Kalhotka ◽  
Petr Doležal
Keyword(s):  
Acetic Acid ◽  
Lactobacillus Plantarum ◽  
Acid Content ◽  
Corn Silage ◽  
Bacterial Strains ◽  
Total Acid ◽  
Bacterial Inoculants ◽  
Aerobic Stability ◽  
Fermentation Quality

In the experiment, the effect of additives supplementation on the fermentation quality of corn silage was examined, compared with the untreated control (K). The aditive „A“ contained bacterial component of (Propionibactrium acidipropionici – MA126/4U 3*1010 and Lactobacillus plantarum – MA18/5U). The effective substances of bacterial inoculants „B“, selected were bacterial strains of (Lactobacillus casei ssp. rhamnosus LC – 705 DSM 7061 4*1011, Propionibacterium freudenreichii spp. shermanii JS DSM 6067 2-4*1011). There were used as effective substances of bacterial inoculants „C“ lactic bacteria and enzyme (Lactobacillus plantarum CCM 3769 1.67*1010, Lactococcus lactis CCM 4754 1.67*1010, Enterococcus faecium CCM 6226 1.67*1010, Pediococcus pentosaceus CCM 3770 1,67*1010, cellulase, hemicellulase, sodium benzoate). They were applied in the dose of prescript by producer. At conservations with all aditivum were statistically significant (P < 0.01) increase of lactic acid formation from 55.31±9.72 g/kg DM of control silage to 59.60±10.84 g/kg DM aditivum „A“, 59.36±10.04 g/ kg DM aditivum „B“ rather to 60.74±9.90 g/kg DM aditivum „C“. Aditives „A“ and „B“ were statistically significant (P < 0.01) increase propoinic acid and total fermentation acid content in silages occured. The fermentation characteristics in the microbial aditivum silages by us were more favourable. The date of fermentation was statistically significant (P < 0.01) increase the contents of acetic acid from 45.49±2.83 g/kg DM of 4st day to 63.07±4.25 g/kg DM of 32ndday rather to 67.70±2.94 g/kg DM of 64st day. There were statistically significant (P < 0.01) increase contents of acetic acid and total acid content. The date of fermentation was statistically significant (P < 0.01) degressive of pH.

Evaluation of Fermentation and Aerobic Stability of Total Mixed Ration Silage Containing Wet Brewers’ Grains and Corn Straw

2011 ◽  
Vol 347-353 ◽  
pp. 189-192
Author(s):  
Hui Li Wang ◽  
Qi Zhong Sun ◽  
Fu Yu Yang ◽  
Chun Cheng Xu
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Propionic Acid ◽  
Air Temperature ◽  
Heat Production ◽  
Lactic Acid Concentration ◽  
Corn Straw ◽  
Total Mixed Ration ◽  
Aerobic Stability ◽  
Fermentation Quality

This experiment was conducted to evaluate the effect of ensiling on fermentation quality and aerobic stability of a total mixed ration (TMR) containing wet brewers’ grains and corn straw. During the ensiling period, pH fell dramatically from 6.00 to 3.92 at the initial 3 days, then it maintained relatively stable. Lactic acid concentration firstly increased rapidly then it became slowly to reach 3.21% at day 28 post-ensiling. No propionic acid or butyric acid was observed throughout the ensiling. When exposed to air, the temperature of TMR increased quickly to reach a maximum of about 45°C at the 6-day, then it tended to decline until day 9. Later, it had another relative low peak at the 10-day, then it dropped slowly to be equal to air temperature. For TMR silage, no heat production or mold were detected in the entire period. In addition, during the days of exposure, the pH for TMR varied from 6.0-8.7, while TMR silages had no significant differences (3.86 to 3.87). The number of lactic acid bacteria (LAB) for the TMR decreased from the initial 3.2×103cfu g-1to below detectable levels and yeast counts increased by 1000 times. However, the TMR silage had no significant change in LAB and yeast counts. These results indicated that the TMR silage showed great quality and aerobic stability. Overall, fermentation plays an important part in helping total mixed ration silage forming a good aerobic stability.

scholarly journals Improvement of Fermentation Quality in the Fermented Total Mixed Ration with Oat Silage

2021 ◽  
Vol 9 (2) ◽  
pp. 420
Author(s):  
Hong Yang ◽  
Bing Wang ◽  
Qing Zhang ◽  
Hui Cheng ◽  
Zhu Yu
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Bacterial Community ◽  
Ammonia Nitrogen ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Nonprotein Nitrogen ◽  
Total Mixed Ration ◽  
Spoilage Yeast ◽  
Fermentation Quality

The use of the fermented total mixed ration (FTMR) is a promising approach for the preservation of homogeneous feed, but changes during fermentation and links with the bacterial community of FTMR are not fully understood. This study investigated the effects of adding oat silage (OS) to the fermented total mixed ration (FTMR) in terms of fermentation, chemical composition, and the bacterial community. The fermentation quality of FTMR with 22% OS was greatly improved, as demonstrated by decreases in the butyric acid concentration, a lower lactic acid/acetic acid ratio, a larger population of lactic acid bacteria (LAB), and quicker spoilage yeast death. Further examination of the effects of various ensiling days on nutritive values showed stable crude protein and nonprotein nitrogen (NPN) contents. The concentrations of acetic acid, propionic acid, and ammonia–nitrogen (NH3–N) were increased following all FTMR treatments after 15 d, while the concentration of water-soluble carbohydrates (WSC) was decreased. More heterofermentative LAB, such as Lentilactobacillus buchneri, Lentilactobacillus brevis, and Companilactobacillus versmoldensis were found after adding 11% and 22% OS. Moreover, the addition of 22% OS caused a marked increase in both bacterial richness and diversity, dominated by the Lactobacillus genus complex. Among species of the Lactobacillus genus complex, the occurrence of Loigolactobacillus coryniformis was positively correlated with lactic acid, NPN, and NH3–N concentrations, suggesting its potential role in altering the fermentation profiles.

scholarly journals Fermentation quality and aerobic stability of Napier grass ensiled with citric acid residue and lactic acid bacteria

2021 ◽  
Vol 9 (1) ◽  
pp. 52-59
Author(s):  
Xuxiong Tao ◽  
Chongwen Ji ◽  
Sifan Chen ◽  
Jie Zhao ◽  
Siran Wang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Citric Acid ◽  
Lactic Acid Bacteria ◽  
Water Soluble ◽  
Neutral Detergent Fiber ◽  
Napier Grass ◽  
Pennisetum Purpureum ◽  
Aerobic Stability ◽  
Fermentation Quality

This study was conducted to investigate the effects of adding citric acid residue (CAR) with or without lactic acid bacteria (LAB) to Napier grass (Cenchrus purpureus; syn. Pennisetum purpureum) cv. Sumu No. 2 at ensiling on the fermentation quality and aerobic stability of the resulting silage. Treatments included: Control (Napier grass forage without additives); and Napier grass inoculated with lactic acid bacteria (Lactobacillus plantarum and L. buchneri) at 1 × 106 cfu/gfresh weight (FW) forage (LAB) or 36 g citric acid residue/kg FW forage (CAR) or a mixture of CAR and LAB (CL). Forty-five days after ensiling the silages were tested for chemical and microbial composition and an aerobic stability test was conducted. The addition of CAR with or without LAB increased the DM and lactic acid concentrations in silage and decreased pH plus acetic acid, ammonia nitrogen (NH3-N), neutral detergent fiber and cellulose concentrations relative to Control. The pH in LAB silage was lower than in Control, while lactic acid concentration was higher. During the first 2 days of aerobic exposure, all additives increased the water-soluble carbohydrate (WSC) and lactic acid concentrations and decreased pH plus NH3-N and acetic acid concentrations. Moreover, CL silages had the highest WSC and the lowest NH3-N and acetic acid concentrations during aerobic exposure. However, all additives failed to improve the aerobic stability of the silage. While CAR with or without LAB inoculant improved the fermentation quality of silage made from Napier grass, more studies are warranted to identify additives which can improve aerobic stability of the silage after opening.

scholarly journals Lentilactobacillus hilgardii Inoculum, Dry Matter Contents at Harvest and Length of Conservation Affect Fermentation Characteristics and Aerobic Stability of Corn Silage

2021 ◽  
Vol 12 ◽  
Author(s):  
Francesco Ferrero ◽  
Ernesto Tabacco ◽  
Giorgio Borreani
Keyword(s):  
Acetic Acid ◽  
Dry Matter ◽  
Corn Silage ◽  
Microbial Count ◽  
Dependent Manner ◽  
Aerobic Stability ◽  
Fermentation Quality ◽  
Silage Quality ◽  
Silage Inoculant ◽  
Positive Effect

Heterofermentative Lentilactobacillus hilgardii isolated from sugarcane silage, has recently been proposed as a silage inoculant to increase aerobic stability. Various conditions can influence the activity of LAB and their ability to alter silage quality (e.g., DM content and length of conservation). The aim of this study has been to evaluate the effect of L. hilgardii on the fermentation quality and aerobic stability of whole crop corn silage with different DM contents (from 26 to 45%), conserved for various conservation lengths (13–272 days). The silages were analyzed for their DM content, pH, fermentative profile, microbial count, and aerobic stability. L. hilgardii showed a positive effect on improving the aerobic stability of silages, due its ability to produce acetic acid, and reduced the yeast count. The acetic acid content increased as the conservation period increased and decreased as the DM content increased. The yeast count was reduced during conservation in a DM dependent manner and the inoculation with LH determined a reduction in the count of 0.48 log cfu/g. The aerobic stability increased as the conservation period increased, and the treatment with LH on average increased the aerobic stability by 19 h. The results of this experiment suggest that higher aerobic stability could be achieved in corn silages by ensiling at medium or low DM contents, or by increasing the length of conservation if a higher DM content at ensiling is needed. The inoculation with LH helps to improve the aerobic stability of corn silages by reducing the yeast count.

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