Identification, ecological evaluation and phylogenetic analysis of non-symbiotic endophytic fungi colonizing timothy grass and perennial ryegrass grown in adjacent plots

2019 ◽  
Vol 74 (1) ◽  
pp. 42-52 ◽  
Author(s):  
Sebastian Wojciech Przemieniecki ◽  
Marta Damszel ◽  
Tomasz Paweł Kurowski ◽  
Jędrzej Mastalerz ◽  
Karol Kotlarz
Keyword(s):  
Phylogenetic Analysis ◽  
Endophytic Fungi ◽  
Perennial Ryegrass ◽  
Ecological Evaluation ◽  
Timothy Grass

scholarly journals EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG

2017 ◽  
Vol 16 (2) ◽  
pp. 115
Author(s):  
Johanna Taribuka ◽  
Christanti Sumardiyono ◽  
Siti Muslimah Widyastuti ◽  
Arif Wibowo
Keyword(s):  
Biological Control ◽  
Phylogenetic Analysis ◽  
Endophytic Fungi ◽  
Trichoderma Harzianum ◽  
Biocontrol Agent ◽  
Biological Control Agent ◽  
Control Agent ◽  
Selective Medium ◽  
Trichoderma Spp ◽  
Its1 And Its2

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.

scholarly journals Incidence of Endophytic Fungi in Perennial Ryegrass in Japan.

1993 ◽  
Vol 59 (2) ◽  
pp. 180-184 ◽  
Author(s):  
Hironori KOGA ◽  
Takashi KIMIGAFUKURO ◽  
Takao TSUKIBOSHI ◽  
Tsutomu UEMATSU
Keyword(s):  
Endophytic Fungi ◽  
Perennial Ryegrass

Phylogenetic analysis and diversity of novel endophytic fungi isolated from medicinal plant Sceletium tortuosum

2018 ◽  
Vol 27 ◽  
pp. 36-43 ◽  
Author(s):  
Madira Coutlyne Manganyi ◽  
Thierry Regnier ◽  
Ajay Kumar ◽  
Cornelius Carlos Bezuidenhout ◽  
Collins Njie Ateba
Keyword(s):  
Phylogenetic Analysis ◽  
Medicinal Plant ◽  
Endophytic Fungi

scholarly journals Increasing the efficiency of cultivation of alfalfa and cereal grass mixtures on eroded slopes

1970 ◽  
pp. 93-102
Author(s):  
V.A. Olifirovych ◽  
Yu.A. Veklenko
Keyword(s):  
Perennial Ryegrass ◽  
Dry Matter ◽  
System Analysis ◽  
Dry Matter Yield ◽  
Botanical Composition ◽  
Forage Production ◽  
Timothy Grass ◽  
Calculation Results ◽  
Grass Stand ◽  
Nitrogen Phosphorus

Purpose. To highlight the features of formation of the botanical composition and the yield of dry matter from alfalfa-cereal and deer vetch-cereal mixtures, depending on the modes of use and fertilization when grown on slopes. To substantiate the technological elements of increasing the efficiency of forage production from slope meadow lands. Methods. System analysis, field, laboratory, comparative calculation. Results. The greatest yield of dry matter in the experiment was formed on the grass mixture of deer vetch with reed fescue and timothy grass during the mowing of the herbage. On average for the 2016-2018 on this grass mixture 8.37 t/ha of dry matter were obtained without fertilization, and 9.52 t/ha – with the application of phosphorus fertilizer at a dose of P60. Less by 0.94-1.14 t/ha the yield of dry matter was obtained from the cut grass stand when using a mixture of alfalfa with reed fescue and timothy grass. With pasture use of herbage, the yield of dry matter from the grass mixture based on deer vetch was also higher compared to the herbage based on alfalfa. So, on the variant without fertilization, in total for four grazing cycles, the dry matter yield from the grass mixture of deer vetch + perennial ryegrass + timothy grass + reed fescue amounted to 5.44 t/ha, and from the grass mixture of alfalfa + perennial ryegrass + timothy grass + reed fescue – 4.86 t/ha. When using nitrogen-phosphorus fertilizers (N90(30+30+30)P60), the yield of dry matter from pasture herbage increased to 6.10 t/ha and 5.90 t ha, respectively. Conclusions. So, the improved elements make it possible to form a cut grass stand on the basis of a mixture of deer vetch with timothy grass and reed fescue with dry matter yield of 9.52 t/ha. In the botanical composition of green mass of the cut grass stand of this mixture, the share of the legume component was 48.2-63.6%. For the pasture use, the grass mixture of deer vetch + perennial ryegrass + timothy grass + reed fescue is recommended, which provides 5.44-6.10 t/ha of dry matter in four grazing cycles.

scholarly journals Comparative Genomics in Perennial Ryegrass (Lolium perenne L.): Identification and Characterisation of an Orthologue for the Rice Plant Architecture-Controlling Gene OsABCG5

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Hiroshi Shinozuka ◽  
Noel O. I. Cogan ◽  
German C. Spangenberg ◽  
John W. Forster
Keyword(s):  
Gene Expression ◽  
Phylogenetic Analysis ◽  
Comparative Genomics ◽  
Gene Family ◽  
Perennial Ryegrass ◽  
Plant Architecture ◽  
Foxtail Millet ◽  
Grass Species ◽  
Plant Type ◽  
Bac Clone

Perennial ryegrass is an important pasture grass in temperate regions. As a forage biomass-generating species, plant architecture-related characters provide key objectives for breeding improvement. In silico comparative genomics analysis predicted colocation between a previously identified QTL for plant type (erect versus prostrate growth) and the ortholocus of the rice OsABCG5 gene (LpABCG5), as well as related QTLs in other Poaceae species. Sequencing of an LpABCG5-containing BAC clone identified presence of a paralogue (LpABCG6) in the vicinity of the LpABCG5 locus, in addition to three other gene-like sequences. Comparative genomics involving five other 5 grass species (rice, Brachypodium, sorghum, maize, and foxtail millet) revealed conserved microsynteny in the ABCG5 ortholocus-flanking region. Gene expression profiling and phylogenetic analysis suggested that the two paralogues are functionally distinct. Fourteen additional ABCG5 gene family members, which may interact with the LpABCG5 gene, were identified through sequencing of transcriptomes from perennial ryegrass leaf, anther, and pistils. A larger-scale phylogenetic analysis of the ABCG gene family suggested conservation between major branches of the Poaceae family. This study identified the LpABCG5 gene as a candidate for the plant type determinant, suggesting that manipulation of gene expression may provide valuable phenotypes for perennial ryegrass breeding.

Endophytic fungi affect growth of perennial ryegrass

1985 ◽  
Vol 28 (1) ◽  
pp. 165-168 ◽  
Author(s):  
G. C. M. Latch ◽  
W. F. Hunt ◽  
D. R. Musgrave
Keyword(s):  
Endophytic Fungi ◽  
Perennial Ryegrass

scholarly journals ASSESSMENT OF GRASS MIXTURES FOR MAKING STABLE LAWNS IN THE CONDITIONS OF THE KIROV REGION

2019 ◽  
Vol 2019 (4) ◽  
pp. 78-88
Author(s):  
Nadezhda Yufereva ◽  
Maria Shchannikova
Keyword(s):  
Perennial Ryegrass ◽  
Weather Conditions ◽  
Single Species ◽  
Perennial Grasses ◽  
Meadow Fescue ◽  
Timothy Grass ◽  
Red Fescue ◽  
Orchard Grass

In this article are presented the results of a field experiment to study grass mixtures based on typical lawn grasses with complementary components. All varieties studied in the experiment are from domestic selection. The experiment was conducted on the experimental field of the Vyatka State Agricultural Academy in Kirov. Weather conditions during the years of this experiment were adverse for perennial grasses. Over the seven years of using of the lawns based on red fescue 'Sigma' and meadow grass 'Dar' with the addition of the perennial ryegrass 'Karat', meadow fescue 'Kvarta' and timothy grass 'VIK 85' were completely transformed into the single-species red fescue herbages (excellent quality) and meadow grass herbages (good quality). In the herbages with the addition of the orchard grass 'Hlynovskaya' share in the seventh year of life was 24–48%. To create high-quality long-term lawns in the conditions of the Kirov region can be recommended grass mixtures based on red fescue 'Sigma' with the addition of the perennial ryegrass, meadow fescue and timothy grass. The quality of meadow grass herbages is lower than that of red fescue, however, from grass mixtures based on meadow grass with the addition of the same complementary components, it is possible to create good quality lawns. The inclusion of the orchard grass in lawn grass mixtures is not recommended, due to the reduction of the quality of lawn herbages.

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