Effects of Lactobacillus buchneri as a silage inoculant or probiotic on in vitro organic matter digestibility, gas production and volatile fatty acids of low dry-matter whole-crop maize silage

2017 ◽  
Vol 72 (3) ◽  
pp. 534-544 ◽  
Author(s):  
C. H. S. Rabelo ◽  
F. C. Basso ◽  
E. C. Lara ◽  
L. G. O. Jorge ◽  
C. J. Härter ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Organic Matter ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Maize Silage ◽  
Lactobacillus Buchneri ◽  
Organic Matter Digestibility ◽  
Silage Inoculant

scholarly journals PENGARUH LAMA WAKTU ENSILASE RUMPUT GAJAH YANG DIBERI MOLASES ATAU LUMPUR KECAP TERHADAP FERMENTABILITAS DAN KECERNAAN IN VITRO

2021 ◽  
Vol 46 (1) ◽  
pp. 53
Author(s):  
Iman Hernaman ◽  
Lukman Purwanto ◽  
Handi Burhanuddin ◽  
Atun Budiman ◽  
Budi Ayuningsih ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Organic Matter ◽  
Incubation Time ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Soy Sauce ◽  
Elephant Grass ◽  
Grass Silage ◽  
Organic Matter Digestibility

This study aimed to evaluate in vitro of Elephant grass silage using molasses and soy sauce sludge as additives with different length of ensilage. This research was conducted experimentally using a completely randomized design (CRD) with eight treatments and three replications. The data collected was carried out by variance and Duncan's test. The elephant grass is carried out by the ensilage process using molasses and soy sauce sludge additives as much as 5% with an incubation time of 3, 6, 9, and 12 weeks. The results showed that the incubation time resulted in the concentration of volatile fatty acids, dry matter, and organic matter digestibility which were significantly different (P <0.05), but not the ammonia (N-NH3) concentration. The use of soy sauce sludge additive resulted in higher volatile fatty acid and dry matter and organic matter digestibility compared to the use of molasses with the average volatile fatty acids (139.21 vs 102.92 mM), dry matter digestibility (73.34 vs 69.18%), and organic matter digestibility (66.47 vs 61.74%). The use of 5% soy sauce sludge with an ensilage duration of 12 weeks resulted in the highest average of volatile fatty acids and dry matter and organic digestibility, respectively, namely 150.67 mM, 76.10%, and 69.26%. In conclusion, the use of soy sauce sludge as much as 5% and 12 weeks of ensilage in making elephant grass silage resulted in the best fermentability and digestibility. 

Optimalisasi Penggunaan Level Sabun Kalsium Minyak Kedelai dalam Ransum Terhadap Karakteristik Fermentasi, Populasi Mikroba dan Kecernaan Nutrien Secara In Vitro Menggunakan Cairan Rumen Sapi Bali

2018 ◽  
Vol 5 (2) ◽  
pp. 11
Author(s):  
Ali Bain ◽  
Komang G. Wiryawan ◽  
Dewi Apri Astuti ◽  
Chairrusyuhur Arman ◽  
Sri Suharti
Keyword(s):  
Fatty Acids ◽  
Organic Matter ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Microbial Population ◽  
Total Volatile ◽  
Calcium Soap ◽  
Total Bacteria ◽  
Range Test

ABSTRAKPenelitian ini bertujuan untuk mengevaluasi optimalisasi penggunaan level sabun kalsium yang berbeda dalam ransum secara in vitro terhadap karakteristik fermentasi, populasi mikroba dan kecernaan nutrien ransum, menggunakan sumber inokulum cairan rumen sapi Bali. Penelitian dilaksanakan menggunakan rancangan acak kelompok dengan 4 jenis perlakuan dalam 3 ulangan.  Ransum penelitian terdiri atas : R1, 40% rumput lapang (RL) + 60% konsentrat (K), R2 (40% RL + 60% K, mengandungn 2.5% SCa-kedelai), R3 (40%  RL + 60% K, mengandung 5% SCa-kedelai), R4 (40%  RL + 60% K, mengandung 7.5% SCa-kedelai). Variabel yang diukur terdiri atas karakteristik fermentasi in vitro (pH, N-NH3, produksi total volatile fatty acids), populasi mikroorganisme (total bakteri dan total protozoa), dan kecernaan nutrien (kecernaan bahan kering dan kecernaan bahan organik). Data dianalisis menggunakan analysis of varians  (ANOVA) dan perbedaan antara perlakuan diuji dengan Duncan’s Multiple Range Test.  Hasil penelitian menunjukkan, penambahan SCa-kedelai pada level yang berbeda dalam konsentrat tidak mempengaruhi pH, konsentrasi amonia, populasi mikroba, kecernaan bahan kering dan kecernaan bahan organik fermentasi tetapi berpengaruh sangat nyata (P<0.002) terhadap produksi total VFA. Produksi total VFA tertinggi diperoleh pada ransum perlakuan R4 (konsentrat yang mengandung 7.5% SCa-kedelai). Produksi total VFA tertinggi diperoleh pada perlakuan R4 dan produksi total VFA paling rendah terjadi pada perlakuan ransum R1 (kontrol).  Produksi total VFA  ransum R1 dan R2 dan ransum R2 dan R3 tidak berbeda nyata namun produksi total VFA ransum R3 lebih tinggi dibanding ransum kontrol. Penambahan SCa-kedelai dalam konsentrat menghasilkan produksi total VFA tetinggi namun mulai menekan populasi total bakteri. Mempertimbangkan hasil peubah fermentasi dan populasi mikroba serta biaya pembuatan produk sabun kalsium, SCa-kedelai pada level 5% merupakan level penggunaan SCa-kedelai yang terbaik dalam ransum.Kata kunci: sabun kalsium, fermentasi, in vitro, kecernaanABSTRACTThe study to evaluate the optimalization the different level of soybean oil calcium soap (CaS-soybean) in ration on in vitro fermentation using Bali cattle rumen fluid. The experiment was arranged in a complete randomized block design with 4 different ration treatments and 3 replicates. Ration treatments were R1: 40% native grass (NG) + 60% concentrate (C), R2 : 40% NG + 60% C, supplemented with 2.5% soybean oil calcium soap (CaS-soybean), and R3 (40% NG + 60% C, supplemented with 5.0% CaS-soybean and R4 (40% NG + 60% C, supplemented with 7.5% CaS-soybean). Variables measured were fermentation characteristics (pH, NH3-N, total volatile fatty acids), microbial population (total bacteria and total protozoa), and nutrient digestibility (dry matter and organic matter digestibility). Data were analyzed using analysis of variance (ANOVA) and the differences between treatments means were examined by Duncan Multiple Range Test. Results of the study  showed that the four different level of CaS-soybean in ration did not have any significant effect (P>0,05) on pH, NH3-N, total bacteria, total protozoa, dry matter and organic matter digestibility. The treatments significantly increased (P<0.05) the production of total volatile fatty acids.  The highest production of total VFA was obtained from ration R4 (concentrate containing 7.5% CaS-soybean) and the lowest was obtained from treatment R1 (control). There were no significant differences between R1 and  R2, and between R2 and R3 on the total VFA production. However, the total productions of VFA in ration R3 were higher than that of the control ration. The addition of CaS-soybean in the concentrate had increased the total production of VFA, but at the same time, it began pressing the total population of bacteria. Considering the results of the fermentation variables and microbial population as well as the cost of making calcium soap products, CaS-soybean at 5% level was selected as the best level of CaS-soybean in ration.Keywords : calcium soap, fermentation, in vitro, digestibility

Comparison of bovine rumen liquor and bovine faeces as sources of micro-organisms for the in vitro gas production technique for assessing silages of maize and maize plant fractions

1998 ◽  
Vol 1998 ◽  
pp. 60-60
Author(s):  
U.R. Altaf ◽  
R. Mauricio ◽  
F.L. Mould ◽  
T. Smith ◽  
E. Owen ◽  
...  
Keyword(s):  
Organic Matter ◽  
Gas Production ◽  
Maize Plant ◽  
Production Technique ◽  
Maize Silage ◽  
Bovine Rumen ◽  
In Vitro Gas Production ◽  
Organic Matter Digestibility ◽  
Micro Organisms

A previous study (Mauricio et al., 1998) with 12 forage substrates (straw, hay and dried grasses) showed a high correlation between rumen liquor and faeces for total gas production and in vitro organic matter digestibility (OMD). However parameters estimated using faecal inoculum were generally lower man when using rumen liquor. To confirm this observation, a second study was conducted using maize silage and silages made from maize plant fractions.

scholarly journals Effects of garlic supplementation on in vitro nutrient digestibility, rumen fermentation, and gas production

2021 ◽  
Vol 51 (2) ◽  
pp. 271-279
Author(s):  
M.R. Kekana ◽  
D. Luseba ◽  
M.C. Muyu
Keyword(s):  
Fatty Acids ◽  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Garlic Powder

Garlic contains secondary metabolites with antimicrobial properties that can alter nutrient digestibility and rumen fermentation, similar to other antimicrobial products. The objectives of the study were to evaluate the effects of garlic powder and garlic juice on in vitro nutrient digestibility, rumen fermentation, and gas production. The treatments consisted of control with no additives, garlic powder, and garlic juice at 0.5 ml and 1 ml. The digestibility of dry matter, crude protein and neutral detergent fibre were determined after 48 hours incubation. Rumen ammonia nitrogen and volatile fatty acids were determined at 12 hours and 24 hours incubation. The cumulative gas production was recorded periodically over 48 hours. The in vitro dry matter disappearance decreased with 1 ml of garlic juice compared with control. The crude protein degradability in garlic powder and garlic juice was lower than in control. Volatile fatty acids increased in all treatments. Individual volatile fatty acids were significantly different, especially propionate, whereas the acetate to propionate ratio was reduced by garlic juice, and ammonia nitrogen was reduced by garlic powder and 0.5 ml of garlic juice. The cumulative gas production increased significantly with both levels of garlic juice. The addition of garlic juice at 0.5 mL/100 ml could enhance the production of propionate, and reduce the acetate to propionate ratio, implying that the supply of hydrogen for methanogens was limited.

Comparison of bovine rumen liquor and bovine faeces as sources of micro-organisms for thein vitrogas production technique for assessing silages of maize and maize plant fractions

1998 ◽  
Vol 1998 ◽  
pp. 60-60 ◽  
Author(s):  
U.R. Altaf ◽  
R. Mauricio ◽  
F.L. Mould ◽  
T. Smith ◽  
E. Owen ◽  
...  
Keyword(s):  
Organic Matter ◽  
Gas Production ◽  
Maize Plant ◽  
Production Technique ◽  
Maize Silage ◽  
Bovine Rumen ◽  
Organic Matter Digestibility ◽  
Micro Organisms

A previous study (Mauricioet al., 1998) with 12 forage substrates (straw, hay and dried grasses) showed a high correlation between rumen liquor and faeces for total gas production andin vitroorganic matter digestibility (OMD). However parameters estimated using faecal inoculum were generally lower man when using rumen liquor. To confirm this observation, a second study was conducted using maize silage and silages made from maize plant fractions.

scholarly journals Fermentabilitas dan Kecernaan Ransum Domba yang Mengandung Limbah Roti secara In Vitro

2019 ◽  
Vol 19 (2) ◽  
pp. 85-90
Author(s):  
Asep Saripudin ◽  
Shena Nurpauza ◽  
Budi Ayuningsih ◽  
Iman Hernaman ◽  
Ana Rochana Tarmidi
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Native Grass ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Close Relationship ◽  
Completely Randomized Design ◽  
Organic Matter Digestibility

ABSTRAK. Penelitian bertujuan untuk mengetahui fermentabilitas dan kecernaan ransum domba yang mengandung limbah roti. Penelitian menggunakan rancangan acak lengkap dengan lima macam ransum perlakuan yang terdiri atas 40% rumput lapangan dan 60% konsentrat yang masing-masing mengandung limbah roti sebanyak 0 (kontrol), 10, 20, 30, dan 40%. Masing-masing perlakuan diulang sebanyak 4 kali dan data yang terkumpul dianalisis dengan analisis sidik ragam dan dilanjutkan dengan uji Duncan. Ransum perlakuan dievaluasi secara in vitro. Peubah yang diukur adalah asam lemak terbang (ALT), N-NH3, kecernaan bahan kering, dan kecernaan bahan organik. Hasil penelitian menunjukkan bahwa penggunaan limbah roti dalam ransum domba sampai 40% mampu meningkatkan konsentrasi ALT (102,63 - 143,88 mM), kecernaan bahan kering (64,66 - 78,61%) dan kecernaan bahan organik (53,41 - 65,82%). Sementara itu, terjadi kenaikan konsentrasi N-NH3 (3,87 - 4,90 mM) sampai penggunaan limbah roti 30%, namun penggunaan 40% limbah roti sama dengan ransum kontrol. Konsentrasi ALT memiliki hubungan erat dengan kecernaan bahan kering dan bahan organik dengan nilai r = 0,67 dan 0,65, pada persamaan regresi Y = 0,1591X + 50,79 dan Y = 0,1528X + 39,619. Kesimpulan, Penggunaan limbah roti di dalam ransum sebanyak 40% tidak mengganggu fermentabilitas dan kecernaan ransum secara in vitro.  (In vitro fermentability and digestibility of sheep rations containing bread waste)ABSTRACT. The study aimed to determine the fermentability and digestibility of sheep rations containing bread waste. The study used a completely randomized design with five types of treatment rations consisting of 40% native grass and 60% concentrate and each treatment containing bread waste as much as 0 (control), 10, 20, 30, and 40%. Each treatment was repeated 4 times and the data collected was analyzed by analysis of variance and continued by Duncan test. The treatment ration was evaluated by in vitro. The variables measured were volatile fatty acids (VFA), N-NH3, dry matter digestibility, and organic matter digestibility. The results showed that the use of bread waste in sheep rations up to 40% was able to increase the concentration of VFA (102.63 to 143.88 mM), dry matter (64.66 to 78.61%) and organic matter digestibility (53.41 to 65.82%). Meanwhile, there was an increase in the concentration of N-NH3 (3.87 to 4.90 mM) until 30%, but the use of 40% bread waste was the same as the control ration. The VFA concentration has a close relationship with the dry matter and organic matter digestibility with values of r = 0.67 and 0.65, in the regression equation Y = 0.1591X + 50.79 and Y = 0.1528X + 39.619. The conclusion is using of bread waste as much as 40% did not disturb the fermentability and digestibility of the ration in vitro.

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