scholarly journals Improved production of bacterial cellulose through investigation of effects of inhibitory compounds from lignocellulosic hydrolysates

GCB Bioenergy ◽  
2020 ◽  
Author(s):  
Haeun Kim ◽  
Jemin Son ◽  
Jonghwa Lee ◽  
Hah Young Yoo ◽  
Taek Lee ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Lignocellulosic Hydrolysates ◽  
Inhibitory Compounds

scholarly journals Cultivation of lipid-producing bacteria with lignocellulosic biomass: Effects of inhibitory compounds of lignocellulosic hydrolysates

2014 ◽  
Vol 161 ◽  
pp. 162-170 ◽  
Author(s):  
Baixin Wang ◽  
Yohannes H. Rezenom ◽  
Kun-Ching Cho ◽  
Janessa L. Tran ◽  
Do Gyun Lee ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Lignocellulosic Hydrolysates ◽  
Inhibitory Compounds

scholarly journals Characterization of Komagataeibacter Isolate Reveals New Prospects on Waste Stream Valorization for Bacterial Cellulose Production

2021 ◽  
Author(s):  
Pietro Cannazza ◽  
Antti Rissanen ◽  
Dieval Guizelini ◽  
Pauli Losoi ◽  
Essi Sarlin ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Minimal Medium ◽  
Crude Glycerol ◽  
Industrial Wastes ◽  
Biodiesel Production ◽  
Acetyl Coa ◽  
Metabolic Intermediate ◽  
Lignocellulosic Hydrolysates ◽  
Production Route ◽  
Yeast Hydrolysate

Komagataeibacter spp. have been used for the bioconversion of industrial wastes and lignocellulosic hydrolysates to bacterial cellulose (BC). Recently studies have demonstrated the capacity of Komagataeibacter spp. in the biotransformation of inhibitors found in lignocellulosic hydrolysates, aromatic lignin-derived monomers (LDMs) and acetate. In general, detoxification and BC synthesis from lignocellulosic inhibitors requires a carbon flow from acetyl-coA towards tricarboxylic acid and gluconeogenesis, respectively. However, the related molecular aspects have not yet been identified in Komagataeibacter spp. In this study, we isolated a cellulose producing bacteria capable of synthesizing BC in a minimal medium containing crude glycerol, a by-product from biodiesel production process. The isolate, affiliated to Komagataeibacter genus, synthesized cellulose in minimal medium containing glucose (3.3±0.3 g/L), pure glycerol (2.2±0.1 g/L) and crude glycerol (2.1±0.1 g/L). Genome assembly and annotation identified four copies of bacterial cellulose synthase operon and genes for redirecting the carbon from central metabolic pathway to gluconeogenesis. According to the genome annotations, a BC production route from acetyl-CoA, a central metabolic intermediate, was hypothesized and was validated using acetate. We identified that when K. rhaeticus ENS9b was grown in minimal medium supplemented with acetate, BC production was not observed. However, in presence of readily utilizable substrate, such as spent yeast hydrolysate, acetate supplementation improved BC synthesis.

Differential effects of major inhibitory compounds from sugarcane-based lignocellulosic hydrolysates on the physiology of yeast strains and lactic acid bacteria

2020 ◽  
Vol 42 (4) ◽  
pp. 571-582 ◽  
Author(s):  
Priscila Cola ◽  
Dielle Pierotti Procópio ◽  
Adriana Tabosa de Castro Alves ◽  
Luciana Rebelo Carnevalli ◽  
Icaro Viana Sampaio ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lignocellulosic Hydrolysates ◽  
Differential Effects ◽  
Yeast Strains ◽  
Inhibitory Compounds

Enzymatic removal of inhibitory compounds from lignocellulosic hydrolysates for biomass to bioproducts applications

2020 ◽  
Vol 36 (11) ◽  
Author(s):  
Robson Tramontina ◽  
Lívia Beatriz Brenelli ◽  
Victoria Sodré ◽  
João Paulo Franco Cairo ◽  
Beatriz Medeiros Travália ◽  
...  
Keyword(s):  
Lignocellulosic Hydrolysates ◽  
Inhibitory Compounds

Effects of inhibitory compounds in lignocellulosic hydrolysates on Mortierella isabellina growth and carbon utilization

2015 ◽  
Vol 183 ◽  
pp. 18-24 ◽  
Author(s):  
Zhenhua Ruan ◽  
Whitney Hollinshead ◽  
Christine Isaguirre ◽  
Yinjie J. Tang ◽  
Wei Liao ◽  
...  
Keyword(s):  
Carbon Utilization ◽  
Lignocellulosic Hydrolysates ◽  
Mortierella Isabellina ◽  
Inhibitory Compounds

Domestication and Screening of Saccharomyces Cerevisiae Strain Resistant to Inhibitors in Lignocellulosic Hydrolysates by Acclimatizing Inhibitory

2013 ◽  
Vol 448-453 ◽  
pp. 1581-1586 ◽  
Author(s):  
Shao Hua Luo ◽  
Yong Wen Huang ◽  
An Chen ◽  
Jia Ling Wang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Parent Strain ◽  
Ethanol Yield ◽  
Lignocellulosic Hydrolysates ◽  
Average Ability ◽  
Good Ability ◽  
Inhibitory Compounds ◽  
Theoretical Ethanol Yield ◽  
New Yeast ◽  
High Ethanol

In order to find the strains which can produce high ethanol yield as well as tolerate inhibitors on the lignocellulosic hydrolysates for developing the renewable bioenergy, the sepecial yeast must be explored. After acclimatizing 23 days and using five different acclimation media with sequential increase in the concentration of inhibitory compounds , a kind ofsaccharomyces cerevisiaestrain resistant to inhibitors was obtained . When the yeast resistant to drug and the parent strain grew in the same media which contained several inhibitory compounds 3.2 g/L acetic acid , 0.8 g/L furfural , 0.4 g/L formic acid , the new yeasts maximal ethanol yield can reach 0.428 g/g , up to 85.6% of theoretical ethanol yield. Compared with drug resistant yeast , the parent strains maximal ethanol production yield only can reach 0.246 g/g , up to 52.8% of theoretical ethanol yield . After 5 continuous ages , the average ability of producing ethanol was stable. Compared with parent strain, the yeast resistant to drug had good ability to ferment glucose and produce ethanol as well as tolerate inhibitors .The new yeast has extensive application prospect in the bioethanol production.

Early Platelet-Collagen Interactions in Whole Blood and Their Modifications by Aspirin and Dipyridamole Evaluated by a New Method (Basic Wave)

1985 ◽  
Vol 54 (04) ◽  
pp. 799-803 ◽  
Author(s):  
José Luís Pérez-Requejo ◽  
Justo Aznar ◽  
M Teresa Santos ◽  
Juana Vallés
Keyword(s):  
Whole Blood ◽  
Ex Vivo ◽  
Platelet Rich Plasma ◽  
White Blood Cells ◽  
Reversible Aggregation ◽  
Inhibitory Compounds ◽  
Rapid Generation ◽  
Stimulation Of ◽  
Collagen Stimulation

SummaryIt is shown that the supernatant of unstirred whole blood at 37° C, stimulated by 1 μg/ml of collagen for 10 sec, produces a rapid generation of pro and antiaggregatory compounds with a final proaggregatory activity which can be detected for more than 60 min on a platelet rich plasma (PRP) by turbidometric aggregometry. A reversible aggregation wave that we have called BASIC wave (for Blood Aggregation Stimulatory and Inhibitory Compounds) is recorded. The collagen stimulation of unstirred PRP produces a similar but smaller BASIC wave. BASIC’s intensity increases if erythrocytes are added to PRP but decreases if white blood cells are added instead. Aspirin abolishes “ex vivo” the ability of whole blood and PRP to generate BASIC waves and dipyridamole “in vitro” significantly reduces BASIC’s intensity in whole blood in every tested sample, but shows little effect in PRP.

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