scholarly journals Elevated carbon dioxide accelerates the spatial turnover of soil microbial communities

2015 ◽  
Vol 22 (2) ◽  
pp. 957-964 ◽  
Author(s):  
Ye Deng ◽  
Zhili He ◽  
Jinbo Xiong ◽  
Hao Yu ◽  
Meiying Xu ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Microbial Communities ◽  
Soil Microbial Communities ◽  
Elevated Carbon Dioxide ◽  
Soil Microbial ◽  
Spatial Turnover

scholarly journals A Rapid Microtiter Plate Method To Measure Carbon Dioxide Evolved from Carbon Substrate Amendments so as To Determine the Physiological Profiles of Soil Microbial Communities by Using Whole Soil

2003 ◽  
Vol 69 (6) ◽  
pp. 3593-3599 ◽  
Author(s):  
Colin D. Campbell ◽  
Stephen J. Chapman ◽  
Clare M. Cameron ◽  
Mitchell S. Davidson ◽  
Jacqueline M. Potts
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Microbial Communities ◽  
Carbon Sources ◽  
Soil Microbial Communities ◽  
Microtiter Plate ◽  
Wastewater Sludge ◽  
Carbon Substrate ◽  
Soil Microbial ◽  
Deep Well

ABSTRACT Sole-carbon-source tests (Biolog), designed to identify bacteria, have become very popular for metabolically fingerprinting soil microbial communities, despite disadvantages associated with the use of carbon source profiles that primarily select for fast-growing bacteria. In this paper we describe the use of an alternative method that combines the advantages of the Biolog community-level physiological profile (CLPP) method, in which microtiter-based detection plates are used, with the ability to measure carbon dioxide evolution from whole soil. This method facilitates measurement over short periods of time (4 to 6 h) and does not require the extraction and culturing of organisms. Deep-well microtiter plates are used as test wells into which soil is placed. The apparatus to fill the deep-well plates and interface it with a second removable detection plate is described. Two detection systems, a simple colorimetric reaction in absorbent alkali and scintillation counting with radioactive carbon sources, are described. The methods were compared to the Biolog-CLPP system by using soils under different vegetation types and soil treated with wastewater sludge. We aimed to test the hypothesis that using whole soil would have specific advantages over using extracts in that more immediate responses to substrates could be obtained that would reflect activity rather than growth. The whole-soil method was more rapid and gave earlier detection of C source use. Also, the metabolic fingerprints obtained could discriminate between sludge treatments.

scholarly journals Elevated Carbon Dioxide Alters the Structure of Soil Microbial Communities

2012 ◽  
Vol 78 (8) ◽  
pp. 2991-2995 ◽  
Author(s):  
Ye Deng ◽  
Zhili He ◽  
Meiying Xu ◽  
Yujia Qin ◽  
Joy D. Van Nostrand ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Microbial Communities ◽  
Microbial Community Composition ◽  
Soil Microbial Communities ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Soil Microbial ◽  
Composition And Structure ◽  
Pyrosequencing Analysis ◽  
Soil Microbial Community Composition

ABSTRACTPyrosequencing analysis of 16S rRNA genes was used to examine impacts of elevated CO2(eCO2) on soil microbial communities from 12 replicates each from ambient CO2(aCO2) and eCO2settings. The results suggest that the soil microbial community composition and structure significantly altered under conditions of eCO2, which was closely associated with soil and plant properties.

Alkaline soil pH affects bulk soil, rhizosphere and root endosphere microbiomes of plants growing in a Sandhills ecosystem

2021 ◽  
Vol 97 (4) ◽  
Author(s):  
Lucas Dantas Lopes ◽  
Jingjie Hao ◽  
Daniel P Schachtman
Keyword(s):  
Microbial Communities ◽  
Plant Species ◽  
Soil Ph ◽  
Soil Microbial Communities ◽  
Bulk Soil ◽  
Strong Impact ◽  
Alkaline Soil ◽  
Alkaline Soils ◽  
Soil Microbial ◽  
Soil Rhizosphere

ABSTRACT Soil pH is a major factor shaping bulk soil microbial communities. However, it is unclear whether the belowground microbial habitats shaped by plants (e.g. rhizosphere and root endosphere) are also affected by soil pH. We investigated this question by comparing the microbial communities associated with plants growing in neutral and strongly alkaline soils in the Sandhills, which is the largest sand dune complex in the northern hemisphere. Bulk soil, rhizosphere and root endosphere DNA were extracted from multiple plant species and analyzed using 16S rRNA amplicon sequencing. Results showed that rhizosphere, root endosphere and bulk soil microbiomes were different in the contrasting soil pH ranges. The strongest impact of plant species on the belowground microbiomes was in alkaline soils, suggesting a greater selective effect under alkali stress. Evaluation of soil chemical components showed that in addition to soil pH, cation exchange capacity also had a strong impact on shaping bulk soil microbial communities. This study extends our knowledge regarding the importance of pH to microbial ecology showing that root endosphere and rhizosphere microbial communities were also influenced by this soil component, and highlights the important role that plants play particularly in shaping the belowground microbiomes in alkaline soils.

LDPE microplastics affect soil microbial communities and nitrogen cycling

2021 ◽  
Vol 773 ◽  
pp. 145640
Author(s):  
Lili Rong ◽  
Longfei Zhao ◽  
Leicheng Zhao ◽  
Zhipeng Cheng ◽  
Yiming Yao ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Nitrogen Cycling ◽  
Soil Microbial Communities ◽  
Soil Microbial

Azorella Cushion Plants and Aridity are Important Drivers of Soil Microbial Communities in Andean Ecosystems

Ecosystems ◽  
2021 ◽  
Author(s):  
Susana Rodríguez-Echeverría ◽  
Manuel Delgado-Baquerizo ◽  
José A. Morillo ◽  
Aurora Gaxiola ◽  
Marlene Manzano ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Soil Microbial Communities ◽  
Soil Microbial ◽  
Cushion Plants
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