scholarly journals Frequency‐specific modification of locomotor components by the white gene in Drosophila melanogaster adult flies

2020 ◽  
Author(s):  
Chengfeng Xiao ◽  
Shuang Qiu
Keyword(s):  
Drosophila Melanogaster ◽  
White Gene ◽  
Specific Modification

scholarly journals Multi-Locus Selection and the Structure of Variation at the white Gene of Drosophila melanogaster

Genetics ◽  
1996 ◽  
Vol 144 (2) ◽  
pp. 635-645 ◽  
Author(s):  
David A Kirby ◽  
Wolfgang Stephan
Keyword(s):  
Drosophila Melanogaster ◽  
White Gene ◽  
Transcriptional Unit ◽  
North American Population ◽  
Evolutionary Mechanisms ◽  
Locus Selection ◽  
Neutral Equilibrium ◽  
History Of ◽  
High Degree ◽  
Very High

Abstract We surveyed sequence variation and divergence for the entire 5972-bp transcriptional unit of the white gene in 15 lines of Drosophila melanogaster and one line of D. simulans. We found a very high degree of haplotypic structuring for the polymorphisms in the 3′ half of the gene, as opposed to the polymorphisms in the 5′ half. To determine the evolutionary mechanisms responsible for this pattern, we sequenced a 1612-bp segment of the white gene from an additional 33 lines of D. melanogaster from a European and a North American population. This 1612-bp segment encompasses an 834bp region of the white gene in which the polymorphisms form high frequency haplotypes that cannot be explained by a neutral equilibrium model of molecular evolution. The small number of recombinants in the 834bp region suggests epistatic selection as the cause of the haplotypic structuring, while an investigation of nucleotide diversity supports a directional selection hypothesis. A multi-locus selection model that combines features from both-hypotheses and takes the recent history of D. melanogaster into account may be the best explanation for these data.

scholarly journals The brown protein of Drosophila melanogaster is similar to the white protein and to components of active transport complexes.

1988 ◽  
Vol 8 (12) ◽  
pp. 5206-5215 ◽  
Author(s):  
T D Dreesen ◽  
D H Johnson ◽  
S Henikoff
Keyword(s):  
Drosophila Melanogaster ◽  
Active Transport ◽  
Compound Eye ◽  
Structural Similarity ◽  
Genomic Region ◽  
White Gene ◽  
Nucleotide Sequencing ◽  
Terminal Portion ◽  
Membrane Components ◽  
Sequence Similarities

The brown gene of Drosophila melanogaster is required for deposition of pteridine pigments in the compound eye and other tissues. We isolated a ca. 150-kilobase region including brown by microdissection and chromosome walking using cosmids. Among the cDNAs identified by hybridization to the cosmids, one class hybridized to a genomic region that is interrupted in two brown mutants, bw and In(2LR)CK, and to 2.8- and 3.0-kilobase poly(A)+ RNAs which are altered in the mutants. Nucleotide sequencing of these cDNAs revealed that the two transcripts differ as a consequence of alternative poly(A) addition and that both encode the same predicted protein of 675 amino acids. Searches of available databases for amino acid sequence similarities detected a striking overall similarity of this predicted protein to that of the D. melanogaster white gene. The N-terminal portion aligned with the HisP family of membrane-associated ATP-binding proteins, most of which are subunits of active transport complexes in bacteria, and to two regions of the multidrug resistance P-glycoprotein. The C-terminal portion showed a structural similarity to integral membrane components of the same complexes. Taken together with earlier biochemical evidence that brown and white gene products are necessary for uptake of a pteridine precursor and genetic evidence that brown and white proteins interact, our results are consistent with suggestions that these proteins are subunits of a pteridine precursor permease.

scholarly journals Local transposition of P elements in Drosophila melanogaster and recombination between duplicated elements using a site-specific recombinase.

Genetics ◽  
1994 ◽  
Vol 137 (2) ◽  
pp. 551-563 ◽  
Author(s):  
K G Golic
Keyword(s):  
Drosophila Melanogaster ◽  
Sister Chromatid ◽  
P Element ◽  
White Gene ◽  
Direct Repeats ◽  
Site Specific ◽  
P Elements ◽  
Dicentric Chromosomes ◽  
A Site ◽  
Different Levels

Abstract The transposase source delta 2-3(99B) was used to mobilize a P element located at sites on chromosomes X, 2 and 3. The transposition event most frequently recovered was a chromosome with two copies of the P element at or near the original site of insertion. These were easily recognized because the P element carried a hypomorphic white gene with a dosage dependent phenotype; flies with two copies of the gene have darker eyes than flies with one copy. The P element also carried direct repeats of the recombination target (FRT) for the FLP site-specific recombinase. The synthesis of FLP in these flies caused excision of the FRT-flanked white gene. Because the two white copies excised independently, patches of eye tissue with different levels of pigmentation were produced. Thus, the presence of two copies of the FRT-flanked white gene could be verified. When the P elements lay in the same orientation, FLP-mediated recombination between the FRTs on separated elements produced deficiencies and duplications of the flanked region. When P elements were inverted, the predominant consequence of FLP-catalyzed recombination between the inverted elements was the formation of dicentric chromosomes and acentric fragments as a result of unequal sister chromatid exchange.

scholarly journals Homology Requirements for Targeting Heterologous Sequences During P-Induced Gap Repair in Drosophila melanogaster  1

Genetics ◽  
1997 ◽  
Vol 147 (2) ◽  
pp. 689-699 ◽  
Author(s):  
Tammy Dray ◽  
Gregory B Gloor
Keyword(s):  
Drosophila Melanogaster ◽  
Point Mutations ◽  
Double Strand Breaks ◽  
Yellow Gene ◽  
P Element ◽  
White Gene ◽  
Base Pairs ◽  
Strand Breaks ◽  
Distal Point ◽  
White Locus

The effect of homology on gene targeting was studied in the context of P-element-induced double-strand breaks at the white locus of Drosophila melanogaster. Double-strand breaks were made by excision of P-whd, a P-element insertion in the white gene. A nested set of repair templates was generated that contained the 8 kilobase (kb) yellow gene embedded within varying amounts of white gene sequence. Repair with unlimited homology was also analyzed. Flies were scored phenotypically for conversion of the yellow gene to the white locus. Targeting of the yellow gene was abolished when all of the 3′ homology was removed. Increases in template homology up to 51 base pairs (bp) did not significantly promote targeting. Maximum conversion was observed with a construct containing 493 bp of homology, without a significant increase in frequency when homology extended to the tips of the chromosome. These results demonstrate that the homology requirements for targeting a large heterologous insertion are quite different than those for a point mutation. Furthermore, heterologous insertions strongly affect the homology requirements for the conversion of distal point mutations. Several aberrant conversion tracts, which arose from templates that contained reduced homology, also were examined and characterized.

scholarly journals Mutations in the white gene of Drosophila melanogaster affecting ABC transporters that determine eye colouration

1999 ◽  
Vol 1419 (2) ◽  
pp. 173-185 ◽  
Author(s):  
Susan M. Mackenzie ◽  
Michael R. Brooker ◽  
Timothy R. Gill ◽  
Graeme B. Cox ◽  
Antony J. Howells ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Abc Transporters ◽  
White Gene

Chromosomal localization of the white gene of Lucilia cuprina (Diptera; Calliphoridae) by in situ hybridization

Genome ◽  
1987 ◽  
Vol 29 (1) ◽  
pp. 72-75 ◽  
Author(s):  
D. G. Bedo ◽  
A. J. Howells
Keyword(s):  
Drosophila Melanogaster ◽  
In Situ Hybridization ◽  
Key Words ◽  
Chromosomal Localization ◽  
Polytene Chromosomes ◽  
White Gene ◽  
Lucilia Cuprina ◽  
Standard Methods ◽  
Cytological Data

The white gene of Lucilia cuprina was mapped to trichogen polytene chromosomes using in situ hybridization. A tritium-labelled riboprobe made from the first gene cloned from this species was used with techniques modified from standard methods used for Drosophila melanogaster. Cytological data limiting the location of the white gene to a small portion of 3L and complementing the in situ results are also presented. Key words: Lucilia cuprina, white gene, in situ hybridization.

Meiotic recombination is suppressed near the histone-defined border of euchromatin and heterochromatin on chromosome 2L of Drosophila melanogaster

Genome ◽  
2016 ◽  
Vol 59 (4) ◽  
pp. 289-294 ◽  
Author(s):  
Alistair B. Coulthard ◽  
Rhodri W. Taylor-Kamall ◽  
Graham Hallson ◽  
Anna Axentiev ◽  
Don A. Sinclair ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Histone Modifications ◽  
Meiotic Recombination ◽  
Pericentric Heterochromatin ◽  
P Element ◽  
White Gene ◽  
Chromosomal Proteins ◽  
Chromatin States ◽  
Genetic Property ◽  
Element Insertion

In Drosophila melanogaster, the borders between pericentric heterochromatin and euchromatin on the major chromosome arms have been defined in various ways, including chromatin-specific histone modifications, the binding patterns of heterochromatin-enriched chromosomal proteins, and various cytogenetic techniques. Elucidation of the genetic properties that independently define the different chromatin states associated with heterochromatin and euchromatin should help refine the boundary. Since meiotic recombination is present in euchromatin, but absent in heterochromatin, it constitutes a key genetic property that can be observed transitioning between chromatin states. Using P element insertion lines marked with a su(Hw) insulated mini-white gene, meiotic recombination was found to transition in a region consistent with the H3K9me2 transition observed in ovaries.

Mosaic suppressor, a gene in Drosophila that modifies retrotransposon expression and interacts with zeste.

Genetics ◽  
1994 ◽  
Vol 136 (2) ◽  
pp. 573-583
Author(s):  
A K Csink ◽  
R Linsk ◽  
J A Birchler
Keyword(s):  
Gene Expression ◽  
Drosophila Melanogaster ◽  
Low Frequency ◽  
Transcript Abundance ◽  
White Gene ◽  
Copia Retrotransposon ◽  
Transcriptional Units ◽  
Regulatory Effects

Abstract A newly identified locus in Drosophila melanogaster, Mosaic suppressor (Msu), is described. This gene modifies the expression of white-apricot (wa), which is a copia retrotransposon-induced allele of the white gene. In addition to suppressing wa in a mosaic fashion, this mutation suppresses or enhances the expression of several other retrotransposon induced white alleles. Mutations in Msu alter copia transcript abundance and may regulate the expression of several other retrotransposons. While each of the two Msu isolates is homozygous lethal, heteroallelic escapers occur at a low frequency. These escapers act not only as strong suppressors of wa, but also as a recessive enhancer of synaptic-dependent gene expression at white. The mutation described here suggests a connection between the regulation of specific transcriptional units such as retrotransposons and more global synapsis dependent regulatory effects.

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