Multiple-stressor effects on stream invertebrates: a mesocosm experiment manipulating nutrients, fine sediment and flow velocity

2016 ◽  
Vol 61 (4) ◽  
pp. 362-375 ◽  
Author(s):  
Vasco Elbrecht ◽  
Arne J. Beermann ◽  
Gunnar Goessler ◽  
Janis Neumann ◽  
Ralph Tollrian ◽  
...  
Keyword(s):  
Flow Velocity ◽  
Fine Sediment ◽  
Mesocosm Experiment ◽  
Stream Invertebrates ◽  
Multiple Stressor

scholarly journals Analyzing multiple stressor effects on EPT taxa in a mesocosm experiment with DNA metabarcoding

2021 ◽  
Vol 4 ◽  
Author(s):  
Marie-Thérése Werner ◽  
Arne Beermann ◽  
Vasco Elbrecht ◽  
Vera Zizka ◽  
Florian Leese
Keyword(s):  
Water Quality ◽  
Flow Velocity ◽  
Fine Sediment ◽  
Sediment Deposition ◽  
Species Level ◽  
Taxonomic Resolution ◽  
Response Patterns ◽  
Multiple Stressor ◽  
Dna Metabarcoding ◽  
Ept Taxa

Multiple stressors diversely and often adversely affect stream ecosystems around the globe. Therefore, understanding multiple stressor effects on different organisms is essential for a better ecosystem understanding, an accurate water quality assessment and improved ecosystem management. However, while multiple stressor effects should be assessed at species level this taxonomic resolution is often not achieved e.g. for stream macroinvertebrates. Due to their high abundance and diversity, species-level identification is often not feasible with morphology‑based approaches. DNA metabarcoding represents an alternative approach for studying multiple stressor interactions at species level. In an outdoor experiment over 10,000 specimens from the insect orders Ephemeroptera, Plecoptera and Trichoptera (EPT), which are routinely used as bioindicators, and their responses to stressors were studied. In the experiment salinity, fine sediment deposition and flow velocity were manipulated in a full‑factorial design in 64 mesocosms with two microhabitats each (streambed and leaf litter), resulting in eight replicates per treatment. DNA metabarcoding revealed 122 EPT Operational Taxonomic Units (OTUs), from which the most abundant 27 alone showed 14 different response patterns to the applied stressors. The high taxonomic resolution achieved by DNA metabarcoding revealed species specific stressor responses that were hidden at a lower taxonomic resolution. As a prominent example, Rhithrogena semicolorata responded negatively to fine sediment deposition and flow velocity reduction, while Ecdyonurus torrentis (both Heptageniidae, Ephemeroptera) was insensitive to experimental manipulation, highlighting different stressor responses among species within the same family (Fig. 1, Beermann et al. 2020). Even for well-studied organisms such as EPT taxa, this study shows that DNA metabarcoding has the potential to depict response patterns at species or OTU level despite high specimen abundance. Consequently, DNA metabarcoding promises to be a rewarding method when investigating and assessing multiple stressor effects on stream water quality.

Individual and combined effects of fine sediment and glyphosate herbicide on invertebrate drift and insect emergence: a stream mesocosm experiment

2016 ◽  
Vol 35 (1) ◽  
pp. 139-151 ◽  
Author(s):  
Francis S. Magbanua ◽  
Colin R. Townsend ◽  
Kimberly J. Hageman ◽  
Jeremy J. Piggott ◽  
Christoph D. Matthaei
Keyword(s):  
Fine Sediment ◽  
Mesocosm Experiment ◽  
Invertebrate Drift ◽  
Combined Effects ◽  
Insect Emergence ◽  
Stream Mesocosm ◽  
Glyphosate Herbicide

Patterns of benthic algae and cyanobacteria along twin-stressor gradients of nutrients and fine sediment: a stream mesocosm experiment

2013 ◽  
Vol 58 (9) ◽  
pp. 1849-1863 ◽  
Author(s):  
Annika Wagenhoff ◽  
Katharina Lange ◽  
Colin R. Townsend ◽  
Christoph D. Matthaei
Keyword(s):  
Benthic Algae ◽  
Fine Sediment ◽  
Mesocosm Experiment ◽  
Stream Mesocosm

Effects of fine sediment addition and removal on stream invertebrates and fish: a reach-scale experiment

2014 ◽  
Vol 59 (12) ◽  
pp. 2584-2604 ◽  
Author(s):  
Javad Ramezani ◽  
Lasse Rennebeck ◽  
Gerard P. Closs ◽  
Christoph D. Matthaei
Keyword(s):  
Fine Sediment ◽  
Stream Invertebrates ◽  
Sediment Addition ◽  
Scale Experiment

Multiple-stressor effects on stream invertebrates: DNA barcoding reveals contrasting responses of cryptic mayfly species

2016 ◽  
Vol 61 ◽  
pp. 159-169 ◽  
Author(s):  
Jan N. Macher ◽  
Romana K. Salis ◽  
Katie S. Blakemore ◽  
Ralph Tollrian ◽  
Christoph D. Matthaei ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Stream Invertebrates ◽  
Multiple Stressor
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