Structure of Pseudomonas aeruginosa spermidine dehydrogenase: a polyamine oxidase with a novel heme‐binding fold

FEBS Journal ◽  
2021 ◽  
Author(s):  
Shiyou Che ◽  
Yakun Liang ◽  
Yujing Chen ◽  
Wenyue Wu ◽  
Ruihua Liu ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Polyamine Oxidase ◽  
Heme Binding

Heme dependent transcriptional regulation of the Pseudomonas aeruginosa tandem sRNA prrF1,F2 locus by the cytoplasmic heme binding protein PhuS

2020 ◽  
Author(s):  
Tyree Wilson ◽  
Susana Mouriño ◽  
Angela Wilks
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fluorescence Anisotropy ◽  
Binding Protein ◽  
Transcriptional Regulator ◽  
Opportunistic Pathogen ◽  
Dual Function ◽  
Heme Binding ◽  
Heme Uptake ◽  
Qpcr Analysis ◽  
Heme Binding Protein

Pseudomonas aeruginosa is an opportunistic pathogen requiring iron for its survival and virulence. P. aeruginosa can acquire iron from heme via the heme assimilation system (Has) and Pseudomonas heme uptake (Phu) systems. The Has and Phu systems have non-redundant roles in heme sensing and transport, respectively. However, despite their respective roles heme taken up by either the Has or Phu system is regulated at the metabolic level by the cytoplasmic heme binding protein PhuS, which controls heme flux through the iron-regulated heme oxygenase HemO. Herein, through a combination of CHIP-PCR, EMSA and fluorescence anisotropy we show PhuS binds upstream of the tandem iron-responsive sRNAs prrF1,F2. Furthermore, qPCR analysis of the PAO1 WT and ΔphuS allelic strain shows loss of PhuS abrogates the heme dependent regulation of PrrH. Taken together our data shows PhuS, in addition to its role in regulating extracellular heme metabolism also functions as a transcriptional regulator of the heme-dependent sRNA, PrrH. This dual function of PhuS is central to integrating extracellular heme utilization into the PrrF/PrrH sRNA regulatory network critical for P. aeruginosa adaptation and virulence within the host.

scholarly journals Crystal structure of the Pseudomonas aeruginosa cytoplasmic heme binding protein, Apo-PhuS

2013 ◽  
Vol 128 ◽  
pp. 131-136 ◽  
Author(s):  
Sarvind Tripathi ◽  
Maura J. O'Neill ◽  
Angela Wilks ◽  
Thomas L. Poulos
Keyword(s):  
Crystal Structure ◽  
Pseudomonas Aeruginosa ◽  
Binding Protein ◽  
Heme Binding ◽  
Heme Binding Protein
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