scholarly journals Function of pH‐dependent transcription factor PacC in regulating development, pathogenicity, and mycotoxin biosynthesis of phytopathogenic fungi

FEBS Journal ◽  
2021 ◽  
Author(s):  
Boqiang Li ◽  
Yong Chen ◽  
Shiping Tian
Keyword(s):  
Transcription Factor ◽  
Phytopathogenic Fungi ◽  
Ph Dependent ◽  
Mycotoxin Biosynthesis

scholarly journals Effect of Environmental pH on Morphological Development of Candida albicans Is Mediated via the PacC-Related Transcription Factor Encoded by PRR2

1999 ◽  
Vol 181 (24) ◽  
pp. 7524-7530 ◽  
Author(s):  
Ana M. Ramon ◽  
Amalia Porta ◽  
William A. Fonzi
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Candida Albicans ◽  
Feedback Loop ◽  
Response Regulator ◽  
Morphological Development ◽  
Alkaline Ph ◽  
Ph Response ◽  
Related Transcription Factor ◽  
Ph Dependent

ABSTRACT The ability to respond to ambient pH is critical to the growth and virulence of the fungal pathogen Candida albicans. This response entails the differential expression of several genes affecting morphogenesis. To investigate the mechanism of pH-dependent gene expression, the C. albicans homolog of pacC, designated PRR2 (for pH response regulator), was identified and cloned. pacC encodes a zinc finger-containing transcription factor that mediates pH-dependent gene expression inAspergillus nidulans. Mutants lacking PRR2 can no longer induce the expression of alkaline-expressed genes or repress acid-expressed genes at alkaline pH. Although the mutation did not affect growth of the cells at acid or alkaline pH, the mutants exhibited medium-conditional defects in filamentation. PRR2was itself expressed in a pH-conditional manner, and its induction at alkaline pH was controlled by PRR1. PRR1 is homologous to palF, a regulator of pacC. Thus,PRR2 expression is controlled by a pH-dependent feedback loop. The results demonstrate that the pH response pathway ofAspergillus is conserved and that this pathway has been adapted to control dimorphism in C. albicans.

scholarly journals pH Signaling in Sclerotinia sclerotiorum: Identification of a pacC/RIM1 Homolog

2001 ◽  
Vol 67 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Jeffrey A. Rollins ◽  
Martin B. Dickman
Keyword(s):  
Transcription Factor ◽  
Oxalic Acid ◽  
Signaling Pathway ◽  
Zinc Finger ◽  
Sclerotinia Sclerotiorum ◽  
Culture Conditions ◽  
Phytopathogenic Fungi ◽  
Molecular Signaling ◽  
Ph Conditions ◽  
Zinc Finger Domains

ABSTRACT Sclerotinia sclerotiorum acidifies its ambient environment by producing oxalic acid. This production of oxalic acid during plant infection has been implicated as a primary determinant of pathogenicity in this and other phytopathogenic fungi. We found that ambient pH conditions affect multiple processes in S. sclerotiorum. Exposure to increasing alkaline ambient pH increased the oxalic acid accumulation independent of carbon source, sclerotial development was favored by acidic ambient pH conditions but inhibited by neutral ambient pH, and transcripts encoding the endopolygalacturonase gene pg1 accumulated maximally under acidic culture conditions. We cloned a putative transcription factor-encoding gene, pac1, that may participate in a molecular signaling pathway for regulating gene expression in response to ambient pH. The three zinc finger domains of the predicted Pac1 protein are similar in sequence and organization to the zinc finger domains of the A. nidulans pH-responsive transcription factor PacC. The promoter of pac1 contains eight PacC consensus binding sites, suggesting that this gene, like its homologs, is autoregulated. Consistent with this suggestion, the accumulation ofpac1 transcripts paralleled increases in ambient pH. Pac1 was determined to be a functional homolog of PacC by complementation of an A. nidulans pacC-null strain with pac1. Our results suggest that ambient pH is a regulatory cue for processes linked to pathogenicity, development, and virulence and that these processes may be under the molecular regulation of a conserved pH-dependent signaling pathway analogous to that in the nonpathogenic fungus A. nidulans.

scholarly journals Ectopic Expression of AhGLK1b (GOLDEN2-like Transcription Factor) in Arabidopsis Confers Dual Resistance to Fungal and Bacterial Pathogens

Genes ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 343
Author(s):  
Niaz Ali ◽  
Hua Chen ◽  
Chong Zhang ◽  
Shahid Ali Khan ◽  
Mamadou Gandeka ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Induced Defense ◽  
Bacterial Pathogens ◽  
Ectopic Expression ◽  
Bacterial Pathogen ◽  
Phytopathogenic Fungi ◽  
Plastid Development ◽  
Reading Frame ◽  
Cultivated Peanut ◽  
Resistance To Infection

GOLDEN2-LIKE (GLK) is a member of the myeloblastosis (MYB) family transcription factor and it plays an important role in the regulation of plastid development and stress tolerance. In this study, a gene named AhGLK1b was identified from a cultivated peanut showing down-regulation in response to low calcium with a complete open reading frame (ORF) of 1212 bp. The AhGLK1b has 99.26% and 96.28% sequence similarities with its orthologs in Arachis ipaensis and A. duranensis, respectively. In the peanut, the AhGLK1b was localized in the nucleus and demonstrated the highest expression in the leaf, followed by the embryo. Furthermore, the expression of AhGLK1b was induced significantly in response to a bacterial pathogen, Ralstonia solanacearum infection. Ectopic expression of AhGLK1b in Arabidopsis showed stronger resistance against important phytopathogenic fungi S. sclerotiorum. It also exhibited high resistance to infection of the bacterial pathogen Pst DC3000. AhGLK1b-expressing Arabidopsis induced defense-related genes including PR10 and Phox/Bem 1 (PBI), which are involved in multiple disease resistance. Taken together, the results suggest that AhGLK1b might be useful in providing dual resistance to fungal and bacterial pathogens as well as tolerance to abiotic stresses.

scholarly journals Identification of a Transcription Factor Controlling pH-Dependent Organic Acid Response in Aspergillus niger

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e50596 ◽  
Author(s):  
Lars Poulsen ◽  
Mikael Rørdam Andersen ◽  
Anna Eliasson Lantz ◽  
Jette Thykaer
Keyword(s):  
Transcription Factor ◽  
Aspergillus Niger ◽  
Organic Acid ◽  
Ph Dependent

scholarly journals Transcription factor control of virulence in phytopathogenic fungi

2021 ◽  
Author(s):  
Evan John ◽  
Karam B. Singh ◽  
Richard P. Oliver ◽  
Kar‐Chun Tan
Keyword(s):  
Transcription Factor ◽  
Phytopathogenic Fungi

scholarly journals Stress-Responsive Alternative Sigma Factor SigB Plays a Positive Role in the Antifungal Proficiency ofBacillus subtilis

2019 ◽  
Vol 85 (9) ◽  
Author(s):  
M. Bartolini ◽  
S. Cogliati ◽  
D. Vileta ◽  
C. Bauman ◽  
W. Ramirez ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Plant Growth ◽  
Plant Defense ◽  
Defense Responses ◽  
Phytopathogenic Fungi ◽  
Plant Diseases ◽  
Phytopathogenic Fungus ◽  
Plant Defense Responses ◽  
Content Type ◽  
Regulatory Pathways

ABSTRACTDifferentBacillusspecies with PGPR (plant growth-promoting rhizobacterium) activity produce potent biofungicides and stimulate plant defense responses against phytopathogenic fungi. However, very little is known about how these PGPRs recognize phytopathogens and exhibit the antifungal response. Here, we report the antagonistic interaction betweenBacillus subtilisand the phytopathogenic fungusFusarium verticillioides. We demonstrate that this bacterial-fungal interaction triggers the induction of the SigB transcription factor, the master regulator ofB. subtilisstress adaptation. Dual-growth experiments performed with live or dead mycelia or culture supernatants ofF. verticillioidesshowed that SigB was activated and required for the biocontrol of fungal growth. Mutations in the different regulatory pathways of SigB activation in the isogenic background revealed that only the energy-related RsbP-dependent arm of SigB activation was responsible for specific fungal detection and triggering the antagonistic response. The activation of SigB increased the expression of the operon responsible for the production of the antimicrobial cyclic lipopeptide surfactin (thesrfAoperon). SigB-deficientB. subtiliscultures produced decreased amounts of surfactin, andB. subtiliscultures defective in surfactin production (ΔsrfA) were unable to control the growth ofF. verticillioides.In vivoexperiments of seed germination efficiency and early plant growth inhibition in the presence ofF. verticillioidesconfirmed the physiological importance of SigB activity for plant bioprotection.IMPORTANCEBiological control using beneficial bacteria (PGPRs) represents an attractive and environment-friendly alternative to pesticides for controlling plant diseases. Different PGPRBacillusspecies produce potent biofungicides and stimulate plant defense responses against phytopathogenic fungi. However, very little is known about how PGPRs recognize phytopathogens and process the antifungal response. Here, we report howB. subtilistriggers the induction of the stress-responsive sigma B transcription factor and the synthesis of the lipopeptide surfactin to fight the phytopathogen. Our findings show the participation of the stress-responsive regulon of PGPRBacillusin the detection and biocontrol of a phytopathogenic fungus of agronomic impact.

scholarly journals Saline stress affects the pH-dependent regulation of the transcription factor PacC in the dermatophyte Trichophyton interdigitale

2020 ◽  
Vol 51 (4) ◽  
pp. 1585-1591
Author(s):  
Larissa Gomes da Silva ◽  
Maíra Pompeu Martins ◽  
Pablo Rodrigo Sanches ◽  
Nalu Teixeira de Aguiar Peres ◽  
Nilce Maria Martinez-Rossi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Saline Stress ◽  
Trichophyton Interdigitale ◽  
Ph Dependent

scholarly journals Histidine switch controlling pH-dependent protein folding and DNA binding in a transcription factor at the core of synthetic network devices

2016 ◽  
Vol 12 (8) ◽  
pp. 2417-2426 ◽  
Author(s):  
D. K. Deochand ◽  
I. C. Perera ◽  
R. B. Crochet ◽  
N. C. Gilbert ◽  
M. E. Newcomer ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Protein Folding ◽  
Dna Binding ◽  
Molten Globule ◽  
Dimer Interface ◽  
Synthetic Network ◽  
The Core ◽  
Dependent Protein ◽  
Ph Dependent

Reversible protonation of histidine at the dimer interface of HucR controls interconversion between molten globule and compact folded state.

Transcription factor/DNA interactions visualized by electron spectroscopic imaging

1992 ◽  
Vol 50 (1) ◽  
pp. 450-451
Author(s):  
David P. Bazett-Jones ◽  
Mark L. Brown
Keyword(s):  
Transcription Factor ◽  
Dna Sequences ◽  
Transcription Initiation ◽  
Molecular Mechanisms ◽  
Phosphorus Content ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
Dna Backbone ◽  
Two Parameters ◽  
High Level

A multisubunit RNA polymerase enzyme is ultimately responsible for transcription initiation and elongation of RNA, but recognition of the proper start site by the enzyme is regulated by general, temporal and gene-specific trans-factors interacting at promoter and enhancer DNA sequences. To understand the molecular mechanisms which precisely regulate the transcription initiation event, it is crucial to elucidate the structure of the transcription factor/DNA complexes involved. Electron spectroscopic imaging (ESI) provides the opportunity to visualize individual DNA molecules. Enhancement of DNA contrast with ESI is accomplished by imaging with electrons that have interacted with inner shell electrons of phosphorus in the DNA backbone. Phosphorus detection at this intermediately high level of resolution (≈lnm) permits selective imaging of the DNA, to determine whether the protein factors compact, bend or wrap the DNA. Simultaneously, mass analysis and phosphorus content can be measured quantitatively, using adjacent DNA or tobacco mosaic virus (TMV) as mass and phosphorus standards. These two parameters provide stoichiometric information relating the ratios of protein:DNA content.

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