PRC1 is a critical regulator for anaphase spindle midzone assembly and cytokinesis in mouse oocyte meiosis

FEBS Journal ◽  
2020 ◽  
Author(s):  
Xiao‐Han Li ◽  
Jia‐Qian Ju ◽  
Zhen‐Nan Pan ◽  
Hong‐Hui Wang ◽  
Xiang Wan ◽  
...  
Keyword(s):  
Mouse Oocyte ◽  
Oocyte Meiosis ◽  
Spindle Midzone

scholarly journals Chromokinesin Kif4 promotes proper anaphase in mouse oocyte meiosis

2019 ◽  
Vol 30 (14) ◽  
pp. 1691-1704 ◽  
Author(s):  
Carissa M. Heath ◽  
Sarah M. Wignall
Keyword(s):  
Family Member ◽  
Chromosome Segregation ◽  
Mouse Oocyte ◽  
Aurora B ◽  
Localization Pattern ◽  
Binding Partner ◽  
Mouse Oocytes ◽  
Oocyte Meiosis ◽  
Spindle Midzone ◽  
Insight Into

Oocytes of many species lack centrioles and therefore form acentriolar spindles. Despite the necessity of oocyte meiosis for successful reproduction, how these spindles mediate accurate chromosome segregation is poorly understood. We have gained insight into this process through studies of the kinesin-4 family member Kif4 in mouse oocytes. We found that Kif4 localizes to chromosomes through metaphase and then largely redistributes to the spindle midzone during anaphase, transitioning from stretches along microtubules to distinct ring-like structures; these structures then appear to fuse together by telophase. Kif4’s binding partner PRC1 and MgcRacGAP, a component of the centralspindlin complex, have a similar localization pattern, demonstrating dynamic spindle midzone organization in oocytes. Kif4 knockdown results in defective midzone formation and longer spindles, revealing new anaphase roles for Kif4 in mouse oocytes. Moreover, inhibition of Aurora B/C kinases results in Kif4 mislocalization and causes anaphase defects. Taken together, our work reveals essential roles for Kif4 during the meiotic divisions, furthering our understanding of mechanisms promoting accurate chromosome segregation in acentriolar oocytes.

scholarly journals Ral GTPase is essential for actin dynamics and Golgi apparatus distribution in mouse oocyte maturation

Cell Division ◽  
2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Ming-Hong Sun ◽  
Lin-Lin Hu ◽  
Chao-Ying Zhao ◽  
Xiang Lu ◽  
Yan-Ping Ren ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Oocyte Maturation ◽  
Polar Body ◽  
Mouse Oocyte ◽  
Actin Dynamics ◽  
Mouse Oocytes ◽  
Oocyte Meiosis ◽  
Ral Gtpase ◽  
Polar Body Extrusion ◽  
Cytoskeleton Dynamics

Abstract Background Ral family is a member of Ras-like GTPase superfamily, which includes RalA and RalB. RalA/B play important roles in many cell biological functions, including cytoskeleton dynamics, cell division, membrane transport, gene expression and signal transduction. However, whether RalA/B involve into the mammalian oocyte meiosis is still unclear. This study aimed to explore the roles of RalA/B during mouse oocyte maturation. Results Our results showed that RalA/B expressed at all stages of oocyte maturation, and they were enriched at the spindle periphery area after meiosis resumption. The injection of RalA/B siRNAs into the oocytes significantly disturbed the polar body extrusion, indicating the essential roles of RalA/B for oocyte maturation. We observed that in the RalA/B knockdown oocytes the actin filament fluorescence intensity was significantly increased at the both cortex and cytoplasm, and the chromosomes were failed to locate near the cortex, indicating that RalA/B regulate actin dynamics for spindle migration in mouse oocytes. Moreover, we also found that the Golgi apparatus distribution at the spindle periphery was disturbed after RalA/B depletion. Conclusions In summary, our results indicated that RalA/B affect actin dynamics for chromosome positioning and Golgi apparatus distribution in mouse oocytes.

FASCIN regulates actin assembly for spindle movement and polar body extrusion in mouse oocyte meiosis

2021 ◽  
Author(s):  
Lin‐Lin Hu ◽  
Meng‐Hao Pan ◽  
Feng‐Lian Yang ◽  
Zi‐Ao Zong ◽  
Feng Tang ◽  
...  
Keyword(s):  
Polar Body ◽  
Mouse Oocyte ◽  
Actin Assembly ◽  
Oocyte Meiosis ◽  
Polar Body Extrusion

scholarly journals OLA1 is responsible for normal spindle assembly and SAC activation in mouse oocytes

2019 ◽  
Author(s):  
Di Xie ◽  
Juan Zhang ◽  
JinLi Ding ◽  
Jing Yang ◽  
Yan Zhang
Keyword(s):  
Spindle Assembly Checkpoint ◽  
Polar Body ◽  
Germinal Vesicle ◽  
Spindle Assembly ◽  
Mouse Oocyte ◽  
Immunofluorescent Staining ◽  
Germinal Vesicle Breakdown ◽  
Oocyte Meiosis ◽  
Polar Body Extrusion ◽  
Spread Analysis

Background. OLA1 is a member of the GTPase protein family, unlike other members, it can bind and hydrolyze ATP more efficiently than GTP. OLA1 participates in cell proliferation, oxidative response and tumorigenesis. However, whether OLA1 is also required for oocyte meiosis is still unknown. Methods. In this study, the localization, expression, and functions of OLA1 in the mouse oocyte meiosis were examined. Immunofluorescent and confocal microscopy were used to explore the location pattern of OLA1 in the mouse oocyte. Moreover, nocodazole treatment was used to confirm the spindle-like location of OLA1 during mouse meiosis. Western blot was used to explore the expression pattern of OLA1 in the mouse oocyte. Microinjection of siRNA was used to explore the OLA1 functions in the mouse oocyte meiosis. In addition, chromosome spreading was used to investigate the spindle assembly checkpoint (SAC) activity. Results. Immunofluorescent staining showed that OLA1 evenly distributed in the cytoplasm at germinal vesicle (GV) stage. After meiosis resumption (GVBD), OLA1 co-localized with spindles, which was further identified by nocodazole treatment experiments. Knockdown of OLA1 impaired the germinal vesicle breakdown progression and finally resulted in a lower polar body extrusion rate. Immunofluorescence analysis indicated that knockdown of OLA1 led to abnormal spindle assembly, which was evidenced by multipolar spindles in OLA1-RNAi-oocytes. After 6 h post-GVBD in culture, an increased proportion of oocyte which has precociously entered into anaphase/telephase I (A/TI) was observed in OLA1-knockdown oocytes, suggesting that loss of OLA1 resulted in the premature segregation of homologous chromosomes. In addition, the chromosome spread analysis suggested that OLA1 knockdown induced premature anaphase onset was due to the precocious inactivation of SAC. Taken together, we concluded that OLA1 plays important role in GVBD, spindle assembly and SAC activation maintenance in oocyte meiosis.

scholarly journals Mps1 kinase-dependent Sgo2 centromere localisation mediates cohesin protection in mouse oocyte meiosis I

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Warif El Yakoubi ◽  
Eulalie Buffin ◽  
Damien Cladière ◽  
Yulia Gryaznova ◽  
Inés Berenguer ◽  
...  
Keyword(s):  
Mouse Oocyte ◽  
Oocyte Meiosis ◽  
Meiosis I

scholarly journals Kif4 Is Essential for Mouse Oocyte Meiosis

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0170650 ◽  
Author(s):  
Nicole J. Camlin ◽  
Eileen A. McLaughlin ◽  
Janet E. Holt
Keyword(s):  
Mouse Oocyte ◽  
Oocyte Meiosis

RAB8A GTPase regulates spindle migration and Golgi apparatus distribution via ROCK-mediated actin assembly in mouse oocyte meiosis†

2018 ◽  
Vol 100 (3) ◽  
pp. 711-720 ◽  
Author(s):  
Zhen-Nan Pan ◽  
Yujie Lu ◽  
Feng Tang ◽  
Meng-Hao Pan ◽  
Xiang Wan ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Mouse Oocyte ◽  
Actin Assembly ◽  
Oocyte Meiosis

Loss of PKC mu function induces cytoskeletal defects in mouse oocyte meiosis

2019 ◽  
Vol 234 (10) ◽  
pp. 18513-18523 ◽  
Author(s):  
Yu Zhang ◽  
Lan‐Lan Wu ◽  
Xiang Wan ◽  
Hong‐Hui Wang ◽  
Xiao‐Han Li ◽  
...  
Keyword(s):  
Mouse Oocyte ◽  
Oocyte Meiosis

Inhibition of HDAC6 by tubastatin A disrupts mouse oocyte meiosis via regulating histone modifications and mRNA expression

2020 ◽  
Vol 235 (10) ◽  
pp. 7030-7042 ◽  
Author(s):  
Liyan Sui ◽  
Rong Huang ◽  
Hao Yu ◽  
Sheng Zhang ◽  
Ziyi Li
Keyword(s):  
Mrna Expression ◽  
Histone Modifications ◽  
Mouse Oocyte ◽  
Oocyte Meiosis

Antioxidants Stimulate Germinal Vesicle Breakdown, But Inhibit Chromosome Formation And Spindle Assembly In Porcine Oocytes

2000 ◽  
Vol 6 (S2) ◽  
pp. 964-965
Author(s):  
Qing-Yuan Sun ◽  
Randall S. Prather ◽  
Heide Schatten
Keyword(s):  
Oocyte Maturation ◽  
Germinal Vesicle ◽  
Spindle Assembly ◽  
Mouse Oocyte ◽  
Germinal Vesicle Breakdown ◽  
Oocyte Meiosis ◽  
First Polar Body ◽  
Porcine Oocyte ◽  
Chromosome Formation

Mammalian oocytes are arrested at the diplotene stage of the first meiotic division. Release of oocytes from their follicles induces meiotic resumption characterized by germinal vesicle breakdown (GVBD), followed by the chromosome formation and metaphase I spindle organization and finally the extrusion the first polar body. Recently it was shown that cellpermeant antioxidants significantly inhibit spontaneous resumption of meiosis in mouse oocytes, which may indicate a role of oxygen radicals in oocyte maturation. The regulation of mouse oocyte meiosis resumption is different from that of large domestic animals in that GVBD is independent of Ca2+ and protein synthesis. The present study investigated the influence of two cell-permeant antioxidants, 2(3)-ter-butyl-4-hydroxyanisole (BHA) and nordihydroguaiaretic acid (NDGA), on porcine oocyte meiosis resumption, chromatin behavior and spindle assembly. Our findings revealed a different role of antioxidants in porcine oocyte meiosis resumption than in mouse oocyte maturation.

Sign in / Sign up

Export Citation Format

Share Document