Quantitative analysis of fluorescent ligand binding to dopamine D3 receptors using live‐cell microscopy

FEBS Journal ◽  
2020 ◽  
Author(s):  
Anni Allikalt ◽  
Tõnis Laasfeld ◽  
Mihkel Ilisson ◽  
Sergei Kopanchuk ◽  
Ago Rinken
Keyword(s):  
Quantitative Analysis ◽  
Ligand Binding ◽  
Live Cell ◽  
Dopamine D3 Receptors ◽  
D3 Receptors ◽  
Live Cell Microscopy ◽  
Dopamine D3 ◽  
Cell Microscopy ◽  
Fluorescent Ligand

scholarly journals Cooperative 4Pi Excitation and Detection Yields Sevenfold Sharper Optical Sections in Live-Cell Microscopy

2004 ◽  
Vol 87 (6) ◽  
pp. 4146-4152 ◽  
Author(s):  
Hilmar Gugel ◽  
Jörg Bewersdorf ◽  
Stefan Jakobs ◽  
Johann Engelhardt ◽  
Rafael Storz ◽  
...  
Keyword(s):  
Live Cell ◽  
Live Cell Microscopy ◽  
Cell Microscopy

Characterization of aripiprazole partial agonist activity at human dopamine D3 receptors

2008 ◽  
Vol 597 (1-3) ◽  
pp. 27-33 ◽  
Author(s):  
Yoshihiro Tadori ◽  
Robert A. Forbes ◽  
Robert D. McQuade ◽  
Tetsuro Kikuchi
Keyword(s):  
Partial Agonist ◽  
Agonist Activity ◽  
Dopamine D3 Receptors ◽  
D3 Receptors ◽  
Partial Agonist Activity ◽  
Dopamine D3

scholarly journals Live-cell microscopy - tips and tools

2009 ◽  
Vol 122 (6) ◽  
pp. 753-767 ◽  
Author(s):  
M. M. Frigault ◽  
J. Lacoste ◽  
J. L. Swift ◽  
C. M. Brown
Keyword(s):  
Live Cell ◽  
Live Cell Microscopy ◽  
Cell Microscopy

scholarly journals ROLE OF DOPAMINE D3 RECEPTORS IN THE PATHOGENESIS OF GENETIC HYPERTENSION• 1635

1997 ◽  
Vol 41 ◽  
pp. 275-275 ◽  
Author(s):  
Laureano D Asico ◽  
Sara Fuchs ◽  
Domenico Accili ◽  
Gilbert M Eisner ◽  
Pedro A Jose
Keyword(s):  
Dopamine D3 Receptors ◽  
D3 Receptors ◽  
Genetic Hypertension ◽  
Dopamine D3

scholarly journals Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy

Biochemistry ◽  
2018 ◽  
Vol 57 (39) ◽  
pp. 5648-5653 ◽  
Author(s):  
Alison G. Tebo ◽  
Frederico M. Pimenta ◽  
Yu Zhang ◽  
Arnaud Gautier
Keyword(s):  
Live Cell ◽  
Chemical Genetic ◽  
Fluorescent Markers ◽  
Live Cell Microscopy ◽  
Cell Microscopy

Dynamics and Traffic for Transfecting Exogenous MicroRNA as Studied by Live-Cell Microscopy

2021 ◽  
Vol 17 (8) ◽  
pp. 1647-1653
Author(s):  
Ke Yang ◽  
Yuanyuan Wang ◽  
Bo Sun ◽  
Tian Tian ◽  
Zhu Dai ◽  
...  
Keyword(s):  
Dynamic Properties ◽  
Mean Square Displacement ◽  
Small Region ◽  
Single Particle Tracking ◽  
Live Cell ◽  
Calculation Results ◽  
Long Time ◽  
Live Cell Microscopy ◽  
Cell Microscopy

MicroRNA (miRNA) has emerged as an important gene-regulator that shows great potential in gene therapy because of its unique roles in gene-regulation. However, the knowledge on their function and transportation in vivo is still lacking, and there are limited obvious evidences to define intracellular transportation of miRNA. In this study, the dynamics of exogenous miR-21 transfected into HeLa cells was traced by live-cell microscopy. Their transportation at key time points was recorded and dynamic properties were analyzed by single particle tracking (SPT) and mean square displacement (MSD) calculation. Results showed that the exogenous miRNAs bounded to cells quickly and went through lysosome into cytosol, where they were subsequently recruited into p-body. They finally were degraded, otherwise went back to cytosol in some way. Long time observation and analysis of motion mode showed that the miRNAs were confined in a small region and their motion modes were flexible in different intracellular microenvironment after entering the cells.

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