A multifunctional DNA polymerase I involves in the maturation of Okazaki fragments during the lagging‐strand DNA synthesis in Helicobacter pylori

FEBS Journal ◽  
2020 ◽  
Author(s):  
Yi‐Wen Cheng ◽  
Cheng‐Yao Chen
Keyword(s):  
Helicobacter Pylori ◽  
Dna Synthesis ◽  
Dna Polymerase ◽  
Dna Polymerase I ◽  
Okazaki Fragments ◽  
Polymerase I ◽  
Lagging Strand

scholarly journals Crystal structures of DNA polymerase I capture novel intermediates in the DNA synthesis pathway

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Nicholas Chim ◽  
Lynnette N Jackson ◽  
Anh M Trinh ◽  
John C Chaput
Keyword(s):  
High Resolution ◽  
Dna Synthesis ◽  
Crystal Structures ◽  
Dna Polymerase ◽  
Active Site ◽  
Dna Polymerase I ◽  
Dynamic Nature ◽  
Enzyme Active Site ◽  
Polymerase I ◽  
In Cells

High resolution crystal structures of DNA polymerase intermediates are needed to study the mechanism of DNA synthesis in cells. Here we report five crystal structures of DNA polymerase I that capture new conformations for the polymerase translocation and nucleotide pre-insertion steps in the DNA synthesis pathway. We suggest that these new structures, along with previously solved structures, highlight the dynamic nature of the finger subdomain in the enzyme active site.

scholarly journals Roles of DNA polymerase I in leading and lagging-strand replication defined by a high-resolution mutation footprint of ColE1 plasmid replication

2011 ◽  
Vol 39 (16) ◽  
pp. 7020-7033 ◽  
Author(s):  
Jennifer M. Allen ◽  
David M. Simcha ◽  
Nolan G. Ericson ◽  
David L. Alexander ◽  
Jacob T. Marquette ◽  
...  
Keyword(s):  
High Resolution ◽  
Dna Polymerase ◽  
Plasmid Replication ◽  
Dna Polymerase I ◽  
Cole1 Plasmid ◽  
Polymerase I ◽  
Lagging Strand

Mechanism of initiation of in vitro DNA synthesis by the immunopurified complex between yeast DNA polymerase I and DNA primase

1986 ◽  
Vol 161 (2) ◽  
pp. 435-440 ◽  
Author(s):  
Gianfranco BADARACCO ◽  
Paola VALSASNINI ◽  
Marco FOIANI ◽  
Roberta BENFANTE ◽  
Giovanna LUCCHINI ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Dna Polymerase I ◽  
Dna Primase ◽  
Polymerase I

scholarly journals DNA polymerases, deoxyribonucleases, and recombination during meiosis in Saccharomyces cerevisiae.

1984 ◽  
Vol 4 (12) ◽  
pp. 2811-2817 ◽  
Author(s):  
M A Resnick ◽  
A Sugino ◽  
J Nitiss ◽  
T Chow
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Synthesis ◽  
Dna Polymerase ◽  
High Efficiency ◽  
Intragenic Recombination ◽  
Dna Polymerase I ◽  
Crude Extracts ◽  
Total Dna ◽  
Polymerase I ◽  
Kinetics Of

We utilized strains of Saccharomyces cerevisiae that exhibit high efficiency of synchrony of meiosis to examine several aspects of meiosis including sporulation, recombination, DNA synthesis, DNA polymerase I and II, and Mg2+-dependent alkaline DNases. The kinetics of commitment to intragenic recombination and sporulation are similar. The synthesis of DNA, as measured directly with diphenylamine, appears to precede the commitment to recombination. Both DNA polymerase I and II activities and total DNA-synthesizing activity in crude extracts increase two- to threefold before the beginning of meiotic DNA synthesis. Increases of 10- to 20-fold over mitotic levels are found for Mg2+-dependent alkaline DNase activity in crude extracts before and during the commitment to meiotic intragenic recombination. Of particular interest is the comparable increase in a nuclease under the control of the RAD52 gene; this enzyme has been identified by the use of antibody raised against a similar enzyme from Neurospora crassa. Since the RAD52 gene is essential for meiotic recombination, the nuclease is implicated in the high levels of recombination observed during meiosis. The effects observed in this report are meiosis specific since they are not observed in an alpha alpha strain.

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