scholarly journals Structural analysis of glycated human hemoglobin using native mass spectrometry

FEBS Journal ◽  
2019 ◽  
Vol 287 (6) ◽  
pp. 1247-1254
Author(s):  
Monita Muralidharan ◽  
Vijay Bhat ◽  
Amit Kumar Mandal
Keyword(s):  
Mass Spectrometry ◽  
Structural Analysis ◽  
Native Mass Spectrometry ◽  
Human Hemoglobin

Structural analysis of human hemoglobin variants by mass spectrometry

1983 ◽  
Vol 48 ◽  
pp. 209-212 ◽  
Author(s):  
Y. Wada ◽  
A. Hayashi ◽  
T. Fujita ◽  
T. Matsuo ◽  
I. Katakuse ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Structural Analysis ◽  
Human Hemoglobin ◽  
Hemoglobin Variants

scholarly journals Expanding the structural analysis capabilities on an Orbitrap-based mass spectrometer for large macromolecular complexes

The Analyst ◽  
2018 ◽  
Vol 143 (1) ◽  
pp. 100-105 ◽  
Author(s):  
Kyle L. Fort ◽  
Michiel van de Waterbeemd ◽  
Dmitriy Boll ◽  
Maria Reinhardt-Szyba ◽  
Mikhail E. Belov ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Structural Analysis ◽  
Mass Spectrometer ◽  
Biological Systems ◽  
Native Mass Spectrometry ◽  
Macromolecular Complexes ◽  
Insight Into

Native mass spectrometry can provide insight into the structure of macromolecular biological systems.

scholarly journals Comparative Structural Analysis of 20S Proteasome Ortholog Protein Complexes by Native Mass Spectrometry

2020 ◽  
Vol 6 (4) ◽  
pp. 573-588 ◽  
Author(s):  
Shay Vimer ◽  
Gili Ben-Nissan ◽  
David Morgenstern ◽  
Fanindra Kumar-Deshmukh ◽  
Caley Polkinghorn ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Structural Analysis ◽  
Protein Complexes ◽  
Native Mass Spectrometry ◽  
20S Proteasome

scholarly journals Structural analysis of glutathionyl hemoglobin using native mass spectrometry

2019 ◽  
Vol 208 (3) ◽  
pp. 107386 ◽  
Author(s):  
Monita Muralidharan ◽  
Amrita Mitra ◽  
Dibyajyoti Maity ◽  
Debnath Pal ◽  
Amit Kumar Mandal
Keyword(s):  
Mass Spectrometry ◽  
Structural Analysis ◽  
Native Mass Spectrometry

Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry

2019 ◽  
Author(s):  
Zachary VanAernum ◽  
Florian Busch ◽  
Benjamin J. Jones ◽  
Mengxuan Jia ◽  
Zibo Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Speed ◽  
Structural Information ◽  
Protein Complexes ◽  
High Sensitivity ◽  
Native Mass Spectrometry ◽  
Structural Features ◽  
Consumer Products ◽  
Cell Lysates ◽  
Protein Expression And Purification

It is important to assess the identity and purity of proteins and protein complexes during and after protein purification to ensure that samples are of sufficient quality for further biochemical and structural characterization, as well as for use in consumer products, chemical processes, and therapeutics. Native mass spectrometry (nMS) has become an important tool in protein analysis due to its ability to retain non-covalent interactions during measurements, making it possible to obtain protein structural information with high sensitivity and at high speed. Interferences from the presence of non-volatiles are typically alleviated by offline buffer exchange, which is timeconsuming and difficult to automate. We provide a protocol for rapid online buffer exchange (OBE) nMS to directly screen structural features of pre-purified proteins, protein complexes, or clarified cell lysates. Information obtained by OBE nMS can be used for fast (<5 min) quality control and can further guide protein expression and purification optimization.

Structural analysis of oligomeric fructans from excised leaves of Lolium temulentum

2020 ◽  
Author(s):  
Ian Sims ◽  
CJ Pollock ◽  
R Horgan
Keyword(s):  
Mass Spectrometry ◽  
Structural Analysis ◽  
Higher Plants ◽  
Water Soluble ◽  
Lolium Temulentum ◽  
Neutral Water ◽  
Alditol Acetates ◽  
Partially Methylated

Individual fructan tri-, tetra- and pentasaccharide isomers in neutral, water-soluble extracts from Lolium temulentum were purified and the linkages present in these isomeric oligosaccharides were analysed by combined GC-mass spectrometry of partially methylated alditol acetates. 1-Kestose and neokestose were the most abundant trisaccharides with 6-kestose present in much lower amounts. Analysis of isomers of DP 4 and 5 showed that multiple linkage types were present with structures based on all three trisaccharides. Oligosaccharides based on neokestose but with 2,6 linkages between adjacent fructose residues have not been previously detected in higher plants. © 1992.

Native Mass Spectrometry-Based Screening for Optimal Sample Preparation in Single Particle Cryo-EM

2020 ◽  
Author(s):  
Paul Dominic B. Olinares ◽  
Jin Young Kang ◽  
Eliza Llewellyn ◽  
Courtney Chiu ◽  
James Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Single Particle ◽  
Native Mass Spectrometry ◽  
Optimal Sample
Sign in / Sign up

Export Citation Format

Share Document