Structural characterization of an on‐pathway intermediate and transition state in the folding of the N‐terminal SH2 domain from SHP2

FEBS Journal ◽  
2019 ◽  
Vol 286 (23) ◽  
pp. 4769-4777 ◽  
Author(s):  
Lorenzo Visconti ◽  
Francesca Malagrinò ◽  
Stefano Gianni ◽  
Angelo Toto
1998 ◽  
Vol 283 (4) ◽  
pp. 893-903 ◽  
Author(s):  
Fabrizio Chiti ◽  
Niccolo’ Taddei ◽  
Nico A.J van Nuland ◽  
Francesca Magherini ◽  
Massimo Stefani ◽  
...  

Author(s):  
Radu Huculeci ◽  
Fabien Kieken ◽  
Abel Garcia-Pino ◽  
Lieven Buts ◽  
Nico van Nuland ◽  
...  

2002 ◽  
Vol 9 (12) ◽  
pp. 900-905 ◽  
Author(s):  
Robert J. Mallis ◽  
Kristine N. Brazin ◽  
D. Bruce Fulton ◽  
Amy H. Andreotti

Author(s):  
Tao Xiao ◽  
Luxin Sun ◽  
Min Zhang ◽  
Zilu Li ◽  
Eric B. Haura ◽  
...  

Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.


2011 ◽  
Vol 44 (06) ◽  
Author(s):  
A Bracher ◽  
C Kozany ◽  
AK Thost ◽  
F Hausch

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
YH Wang ◽  
B Avula ◽  
N Abe ◽  
F Wei ◽  
M Wang ◽  
...  

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