scholarly journals Structural basis for the Target DNA recognition and binding by the MYB domain of phosphate starvation response 1

FEBS Journal ◽  
2019 ◽  
Author(s):  
Meiqin Jiang ◽  
Lifang Sun ◽  
Michail N. Isupov ◽  
Jennifer A. Littlechild ◽  
Xiuling Wu ◽  
...  
Keyword(s):  
Dna Recognition ◽  
Phosphate Starvation ◽  
Structural Basis ◽  
Starvation Response ◽  
Phosphate Starvation Response ◽  
Target Dna ◽  
Myb Domain

scholarly journals Phosphate starvation response controls genes required to synthesize the phosphate analog arsenate

2018 ◽  
Vol 20 (5) ◽  
pp. 1782-1793 ◽  
Author(s):  
Qian Wang ◽  
Yoon-Suk Kang ◽  
Abdullah Alowaifeer ◽  
Kaixiang Shi ◽  
Xia Fan ◽  
...  
Keyword(s):  
Phosphate Starvation ◽  
Starvation Response ◽  
Phosphate Starvation Response

The phosphate-starvation response inVibrio cholerae O1 andphoB mutant under proteomic analysis: Disclosing functions involved in adaptation, survival and virulence

PROTEOMICS ◽  
2006 ◽  
Vol 6 (5) ◽  
pp. 1495-1511 ◽  
Author(s):  
Wanda Maria Almeida von Krüger ◽  
Leticia Miranda Santos Lery ◽  
Marcia Regina Soares ◽  
Fernanda Saloum de Neves-Manta ◽  
Celia Maria Batista e Silva ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Phosphate Starvation ◽  
Starvation Response ◽  
Phosphate Starvation Response

scholarly journals Structural basis for DNA recognition by the transcription regulator MetR

2016 ◽  
Vol 72 (6) ◽  
pp. 417-426 ◽  
Author(s):  
Avinash S. Punekar ◽  
Jonathan Porter ◽  
Stephen B. Carr ◽  
Simon E. V. Phillips
Keyword(s):  
Molecular Level ◽  
Dna Recognition ◽  
Data Driven ◽  
Structural Basis ◽  
Methionine Biosynthesis ◽  
Dna Complexes ◽  
Winged Helix ◽  
Target Dna ◽  
Operator Sequence ◽  
Dna Complex

MetR, a LysR-type transcriptional regulator (LTTR), has been extensively studied owing to its role in the control of methionine biosynthesis in proteobacteria. A MetR homodimer binds to a 24-base-pair operator region of themetgenes and specifically recognizes the interrupted palindromic sequence 5′-TGAA-N5-TTCA-3′. Mechanistic details underlying the interaction of MetR with its target DNA at the molecular level remain unknown. In this work, the crystal structure of the DNA-binding domain (DBD) of MetR was determined at 2.16 Å resolution. MetR-DBD adopts a winged-helix–turn–helix (wHTH) motif and shares significant fold similarity with the DBD of the LTTR protein BenM. Furthermore, a data-driven macromolecular-docking strategy was used to model the structure of MetR-DBD bound to DNA, which revealed that a bent conformation of DNA is required for the recognition helix α3 and the wing loop of the wHTH motif to interact with the major and minor grooves, respectively. Comparison of the MetR-DBD–DNA complex with the crystal structures of other LTTR-DBD–DNA complexes revealed residues that may confer operator-sequence binding specificity for MetR. Taken together, the results show that MetR-DBD uses a combination of direct base-specific interactions and indirect shape recognition of the promoter to regulate the transcription ofmetgenes.

The phosphate-starvation response ofBacillus licheniformis

PROTEOMICS ◽  
2006 ◽  
Vol 6 (12) ◽  
pp. 3582-3601 ◽  
Author(s):  
Le Thi Hoi ◽  
Birgit Voigt ◽  
Britta Jürgen ◽  
Armin Ehrenreich ◽  
Gerhard Gottschalk ◽  
...  
Keyword(s):  
Phosphate Starvation ◽  
Starvation Response ◽  
Phosphate Starvation Response

scholarly journals The effects of soil phosphorous content on microbiota are driven by the plant phosphate starvation response

2019 ◽  
Author(s):  
Omri M. Finkel ◽  
Isai Salas-González ◽  
Gabriel Castrillo ◽  
Stijn Spaepen ◽  
Theresa F. Law ◽  
...  
Keyword(s):  
Indirect Effects ◽  
Plant Immunity ◽  
Phosphate Starvation ◽  
Drop Out ◽  
Microbiota Composition ◽  
Positive Interaction ◽  
Starvation Response ◽  
Soil P ◽  
Phosphate Starvation Response ◽  
Plant Microbiota

AbstractPhosphate starvation response (PSR) in non-mycorrhizal plants comprises transcriptional reprogramming resulting in severe physiological changes to the roots and shoots and repression of plant immunity. Thus, plant-colonizing microorganisms – the plant microbiota – are exposed to direct influence by the soil’s phosphorous (P) content itself, as well as to the indirect effects of soil P on the microbial niches shaped by the plant. The individual contribution of these factors to plant microbiota assembly remains unknown. To disentangle these direct and indirect effects, we planted PSR-deficient Arabidopsis mutants in a long-term managed soil P gradient, and compared the composition of their shoot and root microbiota to wild type plants across different P concentrations. PSR-deficiency had a larger effect on the composition of both bacterial and fungal plant-associated microbiota composition than P concentrations in both roots and shoots. The fungal microbiota was more sensitive to P concentrationsper sethan bacteria, and less depended on the soil community composition.Using a 185-member bacterial synthetic community (SynCom) across a wide P concentration gradient in an agar matrix, we demonstrated a shift in the effect of bacteria on the plant from a neutral or positive interaction to a negative one, as measured by rosette size. This phenotypic shift is accompanied by changes in microbiota composition: the genusBurkholderiais specifically enriched in plant tissue under P starvation. Through a community drop-out experiment, we demonstrate that in the absence ofBurkholderiafrom the SynCom, plant shoots accumulate higher phosphate levels than shoots colonized with the full SynCom, only under P starvation, but not under P-replete conditions. Therefore, P-stressed plants allow colonization by latent opportunistic competitors found within their microbiome, thus exacerbating the plant’s P starvation.

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