scholarly journals Extracellular bone morphogenetic protein modulator BMPER and twisted gastrulation homolog 1 preserve arterial‐venous specification in zebrafish blood vessel development and regulate Notch signaling in endothelial cells

FEBS Journal ◽  
2018 ◽  
Vol 285 (8) ◽  
pp. 1419-1436 ◽  
Author(s):  
Jennifer Susanne Esser ◽  
Rahel Elisabeth Steiner ◽  
Meike Deckler ◽  
Hannah Schmitt ◽  
Bianca Engert ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Vessel ◽  
Notch Signaling ◽  
Bone Morphogenetic Protein ◽  
Twisted Gastrulation ◽  
Blood Vessel Development ◽  
Morphogenetic Protein ◽  
Vessel Development

scholarly journals EHBP1 and EHD2 regulate Dll4 caveolin-mediated endocytosis during blood vessel development

2020 ◽  
Author(s):  
Amelia M Webb ◽  
Caitlin R Francis ◽  
Jayson M Webb ◽  
Hayle Kincross ◽  
Keanna M Lundy ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Plasma Membrane ◽  
Blood Vessel ◽  
Notch Signaling ◽  
Blood Vessel Development ◽  
Absolute Requirement ◽  
Membrane Bound ◽  
Vessel Development ◽  
Notch Activation

ABSTRACTDespite the absolute requirement of Delta/Notch signaling to activate lateral inhibition during early blood vessel development, many mechanisms remain unclear. Here, we identify EHD2 and EHBP1 as novel regulators of Notch activation in endothelial cells through controlling endocytosis of Delta-like ligand 4 (Dll4). Knockout of EHBP1 and EHD2 in zebrafish produced a significant increase in ectopic sprouts in zebrafish intersomitic vessels during development and a reduction in downstream Notch signaling. In vitro, EHBP1 and EHD2 localized to plasma membrane-bound Dll4 and actin independently of clathrin. Disruption of caveolin endocytosis resulted in EHBP1 and EHD2 failing to organize around Dll4 as well as loss of Dll4 internalization in endothelial cells. Overall, we demonstrate that EHBP1 and EHD2 regulate Dll4 endocytosis by anchoring caveolar endocytic pits to the actin cytoskeleton.

The Notch1-Dll4 signaling pathway regulates mouse postnatal lymphatic development

Blood ◽  
2011 ◽  
Vol 118 (7) ◽  
pp. 1989-1997 ◽  
Author(s):  
Kyle Niessen ◽  
Gu Zhang ◽  
John Brady Ridgway ◽  
Hao Chen ◽  
Ganesh Kolumam ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Notch Signaling ◽  
Signaling Pathway ◽  
Lymphatic Vessels ◽  
Mouse Skin ◽  
Notch Signaling Pathway ◽  
Cell Phenotype ◽  
Mural Cell ◽  
Lymphatic Development ◽  
Vessel Development

Abstract The Notch signaling pathway plays a fundamental role during blood vessel development. Notch signaling regulates blood vessel morphogenesis by promoting arterial endothelial differentiation and pro-viding spatial and temporal control over “tip cell” phenotype during angiogenic sprouting. Components of the Notch signaling pathway have emerged as potential regulators of lymphatic development, joining the increasing examples of blood vessel regulators that are also involved in lymphatic development. However, in mammals a role for the Notch signaling pathway during lymphatic development remains to be demonstrated. In this report, we show that blockade of Notch1 and Dll4, with specific function-blocking antibodies, results in defective postnatal lymphatic development in mice. Mechanistically, Notch1-Dll4 blockade is associated with down-regulation of EphrinB2 expression, been shown to be critically involved in VEGFR3/VEGFC signaling, resulting in reduced lymphangiogenic sprouting. In addition, Notch1-Dll4 blockade leads to compromised expression of distinct lymphatic markers and to dilation of collecting lymphatic vessels with reduced and disorganized mural cell coverage. Finally, Dll4-blockade impairs wound closure and severely affects lymphangiogenesis during the wound healing in adult mouse skin. Thus, our study demonstrates for the first time in a mammalian system that Notch1-Dll4 signaling pathway regulates postnatal lymphatic development and pathologic lymphangiogenesis.

scholarly journals Twisted Gastrulation Modulates Bone Morphogenetic Protein-induced Collagen II and X Expression in Chondrocytes in Vitro and in Vivo

2006 ◽  
Vol 281 (42) ◽  
pp. 31790-31800
Author(s):  
Martina Schmidl ◽  
Nadia Adam ◽  
Cordula Surmann-Schmitt ◽  
Takako Hattori ◽  
Michael Stock ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Twisted Gastrulation ◽  
Morphogenetic Protein ◽  
Collagen Ii

scholarly journals Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting Angiogenesis

2022 ◽  
Author(s):  
Caitlin R Francis ◽  
Hayle Kincross ◽  
Erich J Kushner
Keyword(s):  
Blood Vessel ◽  
Actin Polymerization ◽  
Tissue Type ◽  
Potential Contribution ◽  
Rab Gtpases ◽  
Sprouting Angiogenesis ◽  
Apicobasal Polarity ◽  
Blood Vessel Development ◽  
Vessel Development ◽  
Sprout Formation

In early blood vessel development, trafficking programs, such as those using Rab GTPases, are tasked with delivering vesicular cargo with high spatiotemporal accuracy. However, the function of many Rab trafficking proteins remain ill-defined in endothelial tissue; therefore, their relevance to blood vessel development is unknown. Rab35 has been shown to play an enigmatic role in cellular behaviors which differs greatly between tissue-type and organism. Importantly, Rab35 has never been characterized for its potential contribution in sprouting angiogenesis; thus, our goal was to map Rab35s primary function in angiogenesis. Our results demonstrate that Rab35 is critical for sprout formation; in its absence apicobasal polarity is entirely lost in vitro and in vivo. To determine mechanism, we systematically explored established Rab35 effectors and show that none are operative in endothelial cells. However, we find that Rab35 partners with DENNd1c, an evolutionarily divergent guanine exchange factor, to localize to actin. Here, Rab35 regulates actin polymerization, which is required to setup proper apicobasal polarity during sprout formation. Our findings establish that Rab35 is a potent regulator of actin architecture during blood vessel development.

scholarly journals Transcriptomic analysis identifies novel targets for individual bone morphogenetic protein type 1 receptors in endothelial cells

2021 ◽  
Vol 35 (3) ◽  
Author(s):  
Woosoung Choi ◽  
Heon‐Woo Lee ◽  
Boryeong Pak ◽  
Orjin Han ◽  
Minjung Kim ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Bone Morphogenetic Protein ◽  
Transcriptomic Analysis ◽  
Morphogenetic Protein ◽  
Novel Targets
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