scholarly journals Understanding the cancer cell phenotype beyond the limitations of current omics analyses

FEBS Journal ◽  
2015 ◽  
Vol 283 (1) ◽  
pp. 54-73 ◽  
Author(s):  
Rafael Moreno-Sánchez ◽  
Emma Saavedra ◽  
Juan Carlos Gallardo-Pérez ◽  
Franklin D. Rumjanek ◽  
Sara Rodríguez-Enríquez
Keyword(s):  
Cancer Cell ◽  
Cell Phenotype

scholarly journals Tumorigenicity and Validity of Fluorescence Labelled Mesenchymal and Epithelial Human Oral Cancer Cell Lines in Nude Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Wei Xin Cai ◽  
Li Wu Zheng ◽  
Li Ma ◽  
Hong Zhang Huang ◽  
Ru Qing Yu ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Nude Mice ◽  
Tongue Cancer ◽  
Cancer Cell Lines ◽  
Fluorescent Imaging ◽  
Cell Phenotype ◽  
Human Tongue

Tumorigenicity and metastatic activity can be visually monitored in cancer cells that were labelled with stable fluorescence. The aim was to establish and validate local and distant spread of subcutaneously previously injected fluorescence transduced human tongue cancer cell lines of epithelial and mesenchymal phenotype in nude mice. A total of 32 four-week-old male athymic Balb/c nude mice were randomly allocated into 4 groups (n=8). A single dose of 0.3 mL PBS containing 1 × 107 of four different cancer cell-lines (UM1, UM1-GFP, UM2, and UM2-RFP) was injected subcutaneously into the right side of their posterolateral back. Validity assessment of the labelled cancer cells’ tumorigenicity was assessed by physical examination, imaging, and histology four weeks after the injection. The tumor take rate of cancer cells was similar in animals injected with either parental or transduced cancer cells. Transduced cancer cells in mice were easily detectable in vivo and after cryosection using fluorescent imaging. UM1 cells showed increased tumor take rate and mean tumor volume, presenting with disorganized histopathological patterns. Fluorescence labelled epithelial and mesenchymal human tongue cancer cell lines do not change in tumorigenicity or cell phenotype after injection in vivo.

scholarly journals The metabolically-modulated stem cell niche: a dynamic scenario regulating cancer cell phenotype and resistance to therapy

Cell Cycle ◽  
2014 ◽  
Vol 13 (20) ◽  
pp. 3169-3175 ◽  
Author(s):  
Elisabetta Rovida ◽  
Silvia Peppicelli ◽  
Silvia Bono ◽  
Francesca Bianchini ◽  
Ignazia Tusa ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cancer Cell ◽  
Stem Cell Niche ◽  
Cell Phenotype ◽  
Resistance To Therapy ◽  
Dynamic Scenario ◽  
Cell Niche

scholarly journals High-throughput microscopy reveals the impact of multifactorial environmental perturbations on colorectal cancer cell growth

GigaScience ◽  
2021 ◽  
Vol 10 (4) ◽  
Author(s):  
Chun-Te Chiang ◽  
Roy Lau ◽  
Ahmadreza Ghaffarizadeh ◽  
Matthew Brovold ◽  
Dipen Vyas ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Extracellular Matrix ◽  
Cell Growth ◽  
Tumor Microenvironment ◽  
Cancer Cell ◽  
Response To Therapy ◽  
Model Systems ◽  
Cell Phenotype ◽  
Environmental Perturbations ◽  
Drug Concentrations

Abstract Background Colorectal cancer (CRC) mortality is principally due to metastatic disease, with the most frequent organ of metastasis being the liver. Biochemical and mechanical factors residing in the tumor microenvironment are considered to play a pivotal role in metastatic growth and response to therapy. However, it is difficult to study the tumor microenvironment systematically owing to a lack of fully controlled model systems that can be investigated in rigorous detail. Results We present a quantitative imaging dataset of CRC cell growth dynamics influenced by in vivo–mimicking conditions. They consist of tumor cells grown in various biochemical and biomechanical microenvironmental contexts. These contexts include varying oxygen and drug concentrations, and growth on conventional stiff plastic, softer matrices, and bioengineered acellular liver extracellular matrix. Growth rate analyses under these conditions were performed via the cell phenotype digitizer (CellPD). Conclusions Our data indicate that the growth of highly aggressive HCT116 cells is affected by oxygen, substrate stiffness, and liver extracellular matrix. In addition, hypoxia has a protective effect against oxaliplatin-induced cytotoxicity on plastic and liver extracellular matrix. This expansive dataset of CRC cell growth measurements under in situ relevant environmental perturbations provides insights into critical tumor microenvironment features contributing to metastatic seeding and tumor growth. Such insights are essential to dynamical modeling and understanding the multicellular tumor-stroma dynamics that contribute to metastatic colonization. It also establishes a benchmark dataset for training and testing data-driven dynamical models of cancer cell lines and therapeutic response in a variety of microenvironmental conditions.

scholarly journals Cell-Projection Pumping of Fibroblast Contents into Osteosarcoma SAOS-2 Cells Correlates with Increased SAOS-2 Proliferation and Migration, as well as Altered Morphology

Biomolecules ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1875
Author(s):  
Swarna Mahadevan ◽  
James A Cornwell ◽  
Belal Chami ◽  
Elizabeth Kelly ◽  
Hans Zoellner
Keyword(s):  
Cancer Cell ◽  
Cell Tracking ◽  
Time Lapse ◽  
Cell Phenotype ◽  
Phenotypic Change ◽  
Histopathological Diagnosis ◽  
Daughter Cells ◽  
Dividing Cells ◽  
And Migration ◽  
Cell Profile

We earlier reported that cell-projection pumping transfers fibroblast contents to cancer cells and this alters the cancer cell phenotype. Here, we report on single-cell tracking of time lapse recordings from co-cultured fluorescent fibroblasts and SAOS-2 osteosarcoma cells, tracking 5201 cells across 7 experiments. The fluorescent lipophilic marker DiD was used to label fibroblast organelles and to trace the transfer of fibroblast cytoplasm into SAOS-2 cells. We related SAOS-2 phenotypic change to levels of fluorescence transfer from fibroblasts to SAOS-2 cells, as well as what we term ‘compensated fluorescence’, that numerically projects mother cell fluorescence post-mitosis into daughter cells. The comparison of absolute with compensated fluorescence allowed us to deduct if the phenotypic effects in mother SAOS-2 cells were inherited by their daughters. SAOS-2 receipt of fibroblast fluorescence correlated by Kendall’s tau with cell-profile area and without evidence of persistence in daughter cells (median tau = 0.51, p < 0.016); negatively and weakly with cell circularity and with evidence of persistence (median tau = −0.19, p < 0.05); and very weakly with cell migration velocity and without evidence of persistence (median tau = 0.01, p < 0.016). In addition, mitotic SAOS-2 cells had higher rates of prior fluorescence uptake (median = 64.9 units/day) than non-dividing cells (median = 35.6 units/day, p < 0.016) and there was no evidence of persistence post-mitosis. We conclude that there was an appreciable impact of cell-projection pumping on cancer cell phenotype relevant to cancer histopathological diagnosis, clinical spread and growth, with most effects being ‘reset’ by cancer cell mitosis.

scholarly journals Syndecan-2 promotes perineural invasion and cooperates with K-ras to induce an invasive pancreatic cancer cell phenotype

2012 ◽  
Vol 11 (1) ◽  
pp. 19 ◽  
Author(s):  
Tiago De Oliveira ◽  
Ivane Abiatari ◽  
Susanne Raulefs ◽  
Danguole Sauliunaite ◽  
Mert Erkan ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cell ◽  
Perineural Invasion ◽  
Pancreatic Cancer Cell ◽  
Cell Phenotype
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