Protein post-translational modification in host defense: the antimicrobial mechanism of action of human eosinophil cationic protein native forms

FEBS Journal ◽  
2014 ◽  
Vol 281 (24) ◽  
pp. 5432-5446 ◽  
Author(s):  
Vivian A. Salazar ◽  
Jenny Rubin ◽  
Mohammed Moussaoui ◽  
David Pulido ◽  
Maria Victòria Nogués ◽  
...  
Keyword(s):  
Host Defense ◽  
Mechanism Of Action ◽  
Eosinophil Cationic Protein ◽  
Post Translational Modification ◽  
Cationic Protein ◽  
Human Eosinophil ◽  
Antimicrobial Mechanism

scholarly journals Human eosinophil cationic protein. Molecular cloning of a cytotoxin and helminthotoxin with ribonuclease activity.

1989 ◽  
Vol 170 (1) ◽  
pp. 163-176 ◽  
Author(s):  
H F Rosenberg ◽  
S J Ackerman ◽  
D G Tenen
Keyword(s):  
Amino Acid ◽  
Cellular Localization ◽  
Basic Protein ◽  
Eosinophil Cationic Protein ◽  
Ribonuclease Activity ◽  
Monocytic Differentiation ◽  
Cationic Protein ◽  
Human Eosinophil ◽  
Reading Frame ◽  
The Matrix

We have isolated a 725-bp full-length cDNA clone for the human eosinophil cationic protein (ECP). ECP is a small, basic protein found in the matrix of the eosinophil's large specific granule that has cytotoxic, helminthotoxic, and ribonuclease activity, and is a member of the ribonuclease multigene family. The cDNA sequence shows 89% sequence identity with that reported for the related granule protein, eosinophil-derived neurotoxin (EDN). The open reading frame encodes a previously unidentified 27-amino acid leader sequence preceding a 133-residue mature ECP polypeptide with a molecular mass of 15.6 kD. The encoded amino acid sequence of ECP shows 66% identity to that of EDN and 31% identity to that of human pancreatic ribonuclease, including conservation of the essential structural cysteine and cataytic lysine and histidine residues. mRNA for ECP was detected in eosinophil-enriched peripheral granulocytes and in a subclone of the promyelocytic leukemia line, HL-60, induced toward eosinophilic differentiation with IL-5. No ECP mRNA was detected in uninduced HL-60 cells, or in HL-60 cells induced toward monocytic differentiation with vitamin D3 or toward neutrophilic differentiation with DMSO. In contrast, mRNA for EDN was detected in uninduced HL-60 cells and was upregulated in HL-60 cells induced with DMSO. Despite similarities in sequence and cellular localization, these results suggest that ECP and EDN are subject to different regulatory mechanisms.

scholarly journals Kinetic and Product Distribution Analysis of Human Eosinophil Cationic Protein Indicates a Subsite Arrangement That Favors Exonuclease-type Activity

1999 ◽  
Vol 274 (22) ◽  
pp. 15605-15614 ◽  
Author(s):  
Ester Boix ◽  
Zoran Nikolovski ◽  
Gennady P. Moiseyev ◽  
Helene F. Rosenberg ◽  
Claudi M. Cuchillo ◽  
...  
Keyword(s):  
Eosinophil Cationic Protein ◽  
Product Distribution ◽  
Distribution Analysis ◽  
Cationic Protein ◽  
Human Eosinophil ◽  
Type Activity

Radioimmunoassay of Human Eosinophil Cationic Protein

1977 ◽  
Vol 37 (3) ◽  
pp. 331-335 ◽  
Author(s):  
Per Venge ◽  
Lars-Erik Roxin ◽  
Inge Olsson
Keyword(s):  
Eosinophil Cationic Protein ◽  
Cationic Protein ◽  
Human Eosinophil

Three-dimensional crystal structure of human eosinophil cationic protein (RNase 3) at 1.75 Å resolution11Edited by R. Huber

2000 ◽  
Vol 300 (5) ◽  
pp. 1297-1307 ◽  
Author(s):  
Goretti Mallorquı́-Fernández ◽  
Joan Pous ◽  
Rosa Peracaula ◽  
Joan Aymamı́ ◽  
Takashi Maeda ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Three Dimensional ◽  
Eosinophil Cationic Protein ◽  
Cationic Protein ◽  
Human Eosinophil ◽  
Dimensional Crystal

scholarly journals Effects of human eosinophil granule-derived cationic proteins on C-fiber afferents in the rat lung

2001 ◽  
Vol 91 (3) ◽  
pp. 1318-1326 ◽  
Author(s):  
Lu-Yuan Lee ◽  
Qihai Gu ◽  
Gerald J. Gleich
Keyword(s):  
Eosinophil Cationic Protein ◽  
Eosinophil Infiltration ◽  
Cationic Protein ◽  
Human Eosinophil ◽  
Lung Inflation ◽  
Cationic Proteins ◽  
C Fibers ◽  
Arterial Blood ◽  
C Fiber ◽  
Eosinophil Granule

Experiments were performed to test the hypothesis that human eosinophil granule-derived cationic proteins stimulate vagal C-fiber afferents in the lungs and elicit pulmonary chemoreflex responses in anesthetized Sprague-Dawley rats. Intratracheal instillation of eosinophil cationic protein (ECP; 1–2 mg/ml, 0.1 ml) consistently induced an irregular breathing pattern, characterized by tachypnea (change in breathing frequency of 44.7%) and small unstable tidal volume (Vt). The tachypnea, accompanied by decreased heart rate and arterial blood pressure, started within 30 s after the delivery of ECP and lasted for >30 min. These ECP-induced cardiorespiratory responses were completely prevented by perineural capsaicin treatment of both cervical vagi, which selectively blocked C-fiber conduction, suggesting the involvement of these afferents. Indeed, direct recording of single-unit activities of pulmonary C-fibers further demonstrated that the same dose of ECP evoked a pronounced and sustained (>30-min) stimulatory effect on pulmonary C-fibers. Furthermore, the sensitivity of these afferents to lung inflation was also markedly elevated after the ECP instillation, whereas the vehicle of ECP administered in the same manner had no effect. Other types of eosinophil granule cationic proteins, such as major basic protein and eosinophil peroxidase, induced very similar respiratory and cardiovascular reflex responses. In conclusion, these results show that eosinophil granule-derived cationic proteins induce a distinct stimulatory effect on vagal pulmonary C-fiber endings, which may play an important role in the airway hyperresponsiveness associated with eosinophil infiltration in the airways.

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