scholarly journals Magnesium‐dependent folding of a picornavirus IRES element modulates RNA conformation and eIF4G interaction

FEBS Journal ◽  
2014 ◽  
Vol 281 (16) ◽  
pp. 3685-3700 ◽  
Author(s):  
Gloria Lozano ◽  
Noemi Fernandez ◽  
Encarnacion Martinez‐Salas
Keyword(s):  
Rna Conformation ◽  
Ires Element

Contribution of RNA Conformation to the Stability of a High-Affinity RNA−Protein Complex

2000 ◽  
Vol 122 (29) ◽  
pp. 7130-7131 ◽  
Author(s):  
Sarah J. Luchansky ◽  
Scott J. Nolan ◽  
Anne M. Baranger
Keyword(s):  
Protein Complex ◽  
High Affinity ◽  
Rna Conformation ◽  
The Stability

scholarly journals Effect of 2-Thiouridine on RNA Conformation

2018 ◽  
Author(s):  
A.K. Sarkar ◽  
J. Sarzynska ◽  
A. Lahiri
Keyword(s):  
Rna Conformation

scholarly journals Sensitive measurement of single-nucleotide polymorphism-induced changes of RNA conformation: application to disease studies

2012 ◽  
Vol 41 (1) ◽  
pp. 44-53 ◽  
Author(s):  
Raheleh Salari ◽  
Chava Kimchi-Sarfaty ◽  
Michael M. Gottesman ◽  
Teresa M. Przytycka
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Rna Conformation ◽  
Induced Changes

scholarly journals Click Dimers To Target HIV TAR RNA Conformation

Biochemistry ◽  
2012 ◽  
Vol 51 (11) ◽  
pp. 2331-2347 ◽  
Author(s):  
Sunil Kumar ◽  
Patrick Kellish ◽  
W. Edward Robinson ◽  
Deyun Wang ◽  
Daniel H. Appella ◽  
...  
Keyword(s):  
Rna Conformation ◽  
Tar Rna

scholarly journals Analysis of r-protein and RNA conformation of 30S subunit intermediates in bacteria

RNA ◽  
2015 ◽  
Vol 21 (7) ◽  
pp. 1323-1334 ◽  
Author(s):  
Nathan Napper ◽  
Gloria M. Culver
Keyword(s):  
Rna Conformation ◽  
30S Subunit

scholarly journals Functional interaction of translation initiation factor eIF4G with the foot-and-mouth disease virus internal ribosome entry site

2001 ◽  
Vol 82 (4) ◽  
pp. 757-763 ◽  
Author(s):  
Lanja Saleh ◽  
René C. Rust ◽  
Ralf Füllkrug ◽  
Ewald Beck ◽  
Gergis Bassili ◽  
...  
Keyword(s):  
Translation Initiation ◽  
Foot And Mouth Disease ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Entry Site ◽  
Stem Loop ◽  
Internal Ribosome Entry ◽  
Initiation Factors ◽  
Mouth Disease Virus ◽  
Ires Element

In the life-cycle of picornaviruses, the synthesis of the viral polyprotein is initiated cap-independently at the internal ribosome entry site (IRES) far downstream from the 5′ end of the viral plus-strand RNA. The cis-acting IRES RNA elements serve as binding sites for translation initiation factors that guide the ribosomes to an internal site of the viral RNA. In this study, we show that the eukaryotic translation initiation factor eIF4G interacts directly with the IRES of foot-and-mouth disease virus (FMDV). eIF4G binds mainly to the large Y-shaped stem–loop 4 RNA structure in the 3′ region of the FMDV IRES element, whereas stem–loop 5 contributes only slightly to eIF4G binding. Two subdomains of stem–loop 4 are absolutely essential for eIF4G binding, whereas another subdomain contributes to a lesser extent to binding of eIF4G. At the functional level, the translational activity of stem–loop 4 subdomain mutants correlates with the efficiency of binding of eIF4G in the UV cross-link assay. This indicates that the interaction of eIF4G with the IRES is crucial for the initiation of FMDV translation. A model for the interaction of initiation factors with the IRES element is discussed.

scholarly journals Correction to Click Dimers To Target HIV TAR RNA Conformation

Biochemistry ◽  
2013 ◽  
Vol 52 (40) ◽  
pp. 7159-7159
Author(s):  
Sunil Kumar ◽  
Patrick Kellish ◽  
W. Edward Robinson ◽  
Deyun Wang ◽  
Daniel H. Appella ◽  
...  
Keyword(s):  
Rna Conformation ◽  
Tar Rna
Sign in / Sign up

Export Citation Format

Share Document