Biochemical, histopathological and behavioral alterations caused by intrastriatal administration of quinolic acid to young rats

FEBS Journal ◽  
2014 ◽  
Vol 281 (8) ◽  
pp. 2061-2073 ◽  
Author(s):  
Paula Pierozan ◽  
Carolina G. Fernandes ◽  
Márcio F. Dutra ◽  
Pablo Pandolfo ◽  
Fernanda Ferreira ◽  
...  
Keyword(s):  
Behavioral Alterations ◽  
Young Rats ◽  
Intrastriatal Administration

Behavioral alterations of young rats with a history of oversedation at birth

Neurology ◽  
1964 ◽  
Vol 14 (6) ◽  
pp. 510-510 ◽  
Author(s):  
R. F. Becker ◽  
C. A. Boneau ◽  
C. A. Shearin ◽  
J. E. King
Keyword(s):  
Behavioral Alterations ◽  
Young Rats ◽  
History Of

Behavioral alterations in the short and long term after chronic exposure of silver nanoparticles to young rats

2018 ◽  
Vol 295 ◽  
pp. S204-S205
Author(s):  
J. Orzelska-Gorka ◽  
L. Struzynska ◽  
S. Talarek ◽  
E. Kedzierska ◽  
G. Biala
Keyword(s):  
Silver Nanoparticles ◽  
Chronic Exposure ◽  
Behavioral Alterations ◽  
Young Rats ◽  
Short And Long Term

scholarly journals Early Neurodevelopmental Anomalies in Young Rats from Adult female Treated with Valproic Acid

2021 ◽  
Vol 9 (2) ◽  
pp. 75
Author(s):  
Landry Martial Miguel ◽  
Archange Emmanuel Mboungou Malonga ◽  
Didier Gesril Njilo Tchatchouang ◽  
Childérick Lékana ◽  
Choupette Ravelle Dobhat-Doukakini ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Motor Coordination ◽  
Performance Testing ◽  
Female Rats ◽  
Success Rates ◽  
Behavioral Alterations ◽  
Adult Rats ◽  
Developmental Abnormalities ◽  
Sensorimotor Development ◽  
Young Rats

Background: the influence of VPA on murine fertility, and on offspring is well documented: VPA decreases the fertility rate (by 25%) and the number of fœtus. Furthermore, VPA causes behavioral alterations in rodents similar to the symptoms observed in autism.Objective: in this study we investigated the effects of exposure of non-pregnant adult rats to VPA in the offspring of these animals.Material and methods: non-pregnant adult rats were divided into 3 groups; (1) distilled water group, (2) VPA 200 mg / kg group and (3) VPA 400 mg/kg group. The products were administered orally daily for 30 days. At the end of treatments, all rats were put into monogamous mating with breeding males. The zootechnical characteristics (gestation period, litter size, mortality rate) were then noted. The young rats were then subjected to a battery of behavioral tests (reversal and anti-gravity reflexes, cliff avoidance, suspension, motor coordination and eye opening), carried out at different stages of life to assess sensorimotor development. Morphological abnormalities were also sought, as well as the mortality rate on the 28th day of life.Results: An increase in the mortality rate and a decrease in the mean lifespan were found in female rats exposed to VPA. Young rats from female rats exposed to VPA showed decreased success rates and performance in behavioral testing. Morphodevelopmental abnormalities such as adictalia or stump necrosis were found in the VPA groups. The offspring mortality rate of female rats exposed to VPA 200 mg/kg was 100%.Conclusion: VPA administered to non-pregnant adult rats causes developmental abnormalities, decreased success rates for performance testing, deformities and increased mortality in young rats from the treated rats by VPA.  

On the Fibrinolytic System in Aged Rats, and Its Reactivity to Endotoxin and Cytokines

1992 ◽  
Vol 67 (06) ◽  
pp. 697-701 ◽  
Author(s):  
J J Emeis ◽  
A Brouwer ◽  
R J Barelds ◽  
M A Horan ◽  
S K Durham ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Fibrinolytic System ◽  
Tissue Type ◽  
High Dose ◽  
Base Line ◽  
Aged Rats ◽  
Tissue Type Plasminogen Activator ◽  
Young Rats ◽  
Type Plasminogen Activator

SummaryAged rats are more susceptible to endotoxin-induced effects, including microthrombosis and platelet aggregation, than are young rats. To investigate whether changes in the fibrinolytic system might be involved, we investigated the fibrinolytic activity in plasma euglobulin fractions and tissues (lung and heart) of young (6-months old) and aged (24-months old) rats under baseline conditions and after challenge with endotoxin. Aged rats had lower plasma levels of tissue-type plasminogen activator (t-PA) and of urokinase-type PA (u-PA) activity. PA inhibitor (PAI) activity was higher in the plasma of aged rats, as was t-PA activity in lung and heart.Rats were treated with either a low dose (1 μg/kg) or a high dose (10 mg/kg) of endotoxin. Both treatments induced a transient phase of increased blood fibrinolytic activity, as evidenced by higher levels of tissue-type plasminogen activator (t-PA) activity and decreased levels of PA inhibitor (PAI) activity. Over time, the fibrinolytic activity decreased, probably due to increased levels of PA inhibitor.Both the early increase in t-PA activity, and the subsequent increase in PAI activity, were more pronounced in the aged rats, as compared with the younger rats, after the high dose of endotoxin. The aged rats also responded to an injection of interleukin-1β or tumor necrosis factor-α with a larger increase of PAI activity than did the younger rats.Together the data suggest that, compared to young rats, aged rats have a decreased base-line plasma fibrinolytic activity, while their fibrinolytic system is more responsive to challenge by endotoxin and cytokines.

Effect of KH-305 on the Nitric Oxide Synthase Activity and Erectile Dysfunction in Young Rats

2007 ◽  
Vol 36 (3) ◽  
pp. 305-310 ◽  
Author(s):  
Eun-Jeong Lee ◽  
Hee-Seok Kim ◽  
Byoung-Chul Kim ◽  
Sung-Wan Hwang ◽  
Sung-Yeoun Hwang
Keyword(s):  
Nitric Oxide ◽  
Erectile Dysfunction ◽  
Nitric Oxide Synthase ◽  
Young Rats ◽  
Oxide Synthase ◽  
Nitric Oxide Synthase Activity

Evaluation of the In Vivo Acute Toxicity and In Vitro Hemolytic and Immunomodulatory Activities of the Moringa oleifera Flower Trypsin Inhibitor (MoFTI)

2020 ◽  
Vol 27 ◽  
Author(s):  
Leydianne Leite de Siqueira Patriota ◽  
Dayane Kelly Dias do Nascimento Santos ◽  
Bárbara Rafaela da Silva Barros ◽  
Lethícia Maria de Souza Aguiar ◽  
Yasmym Araújo Silva ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Trypsin Inhibitor ◽  
Hemolytic Activity ◽  
Moringa Oleifera ◽  
Biological Activities ◽  
Hematological Parameters ◽  
Behavioral Alterations ◽  
Female Mice

Background: Protease inhibitors have been isolated from plants and present several biological activities, including immunomod-ulatory action. Objective: This work aimed to evaluate a Moringa oleifera flower trypsin inhibitor (MoFTI) for acute toxicity in mice, hemolytic activity on mice erythrocytes and immunomodulatory effects on mice splenocytes. Methods: The acute toxicity was evaluated using Swiss female mice that received a single dose of the vehicle control or MoFTI (300 mg/kg, i.p.). Behavioral alterations were observed 15–240 min after administration, and survival, weight gain, and water and food consumption were analyzed daily. Organ weights and hematological parameters were analyzed after 14 days. Hemolytic activity of MoFTI was tested using Swiss female mice erythrocytes. Splenocytes obtained from BALB/c mice were cultured in the absence or presence of MoFTI for the evaluation of cell viability and proliferation. Mitochondrial membrane potential (ΔΨm) and reactive oxygen species (ROS) levels were also determined. Furthermore, the culture supernatants were analyzed for the presence of cytokines and nitric oxide (NO). Results: MoFTI did not cause death or any adverse effects on the mice except for abdominal contortions at 15–30 min after administration. MoFTI did not exhibit a significant hemolytic effect. In addition, MoFTI did not induce apoptosis or necrosis in splenocytes and had no effect on cell proliferation. Increases in cytosolic and mitochondrial ROS release, as well as ΔΨm reduction, were observed in MoFTI-treated cells. MoFTI was observed to induce TNF-α, IFN-γ, IL-6, IL-10, and NO release. Conclusion: These results contribute to the ongoing evaluation of the antitumor potential of MoFTI and its effects on other immunological targets.

Deglucohellebrin: A Potent Agent for Glioblastoma Treatment

2020 ◽  
Vol 20 (1) ◽  
pp. 103-110
Author(s):  
Evrysthenis Vartholomatos ◽  
George A. Alexiou ◽  
Georgios S. Markopoulos ◽  
Diamanto Lazari ◽  
Olga Tsiftsoglou ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Primary Brain Tumor ◽  
Behavioral Alterations ◽  
Endemic Plant ◽  
M Cell ◽  
Apoptotic Pathway ◽  
Cycle Arrest ◽  
Dismal Prognosis ◽  
Mitochondrial Membrane Depolarization ◽  
Induced Changes

Background: Glioblastoma is the most common primary brain tumor in adults with a dismal prognosis. To date, several anticancer agents have been isolated from plants. Helleborus odorus subsp. Cyclophyllus is an endemic plant of the Balcan flora. Herewith, we investigated for the first time, the anti-glioma effect of deglucohellebrin (DGH) extracted from the roots of Helleborus. Methods: We investigated the effect of DGH in U251MG, T98G and U87G glioblastoma cell lines. We selected the T98G cells because of their inherent temozolomide resistance. Results: The IC50 value of reduced viability for DGH was 7x10-5M in U251MG cells, 5x10-5M for the T98G cells and 4x10-5M in U87G cells during 72h treatment. DGH induced G2/M cell cycle arrest, caspace-8 activation and significant mitochondrial membrane depolarization, suggesting the activation of the intrinsic, mitochondrial- dependent apoptotic pathway. DGH and temozolomide induced changes in CDs’ expression in U251MG and T98G cells. In zebrafish, DGH did not induce toxicity or behavioral alterations. Conclusion: The present study is the first to determine the anti-glioma activity of DGH. DGH may be a potent agent for glioblastoma treatment and further studies are needed.

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