scholarly journals Mechanism of 3D domain swapping in bovine seminal ribonuclease

FEBS Journal ◽  
2013 ◽  
Vol 281 (3) ◽  
pp. 842-850 ◽  
Author(s):  
Roberta Spadaccini ◽  
Carmine Ercole ◽  
Giuseppe Graziano ◽  
Rainer Wechselberger ◽  
Rolf Boelens ◽  
...  
Keyword(s):  
Domain Swapping ◽  
Bovine Seminal Ribonuclease

scholarly journals Binding of a substrate analog to a domain swapping protein: X-ray structure of the complex of bovine seminal ribonuclease with uridylyl(2′,5′)adenosine

1998 ◽  
Vol 7 (8) ◽  
pp. 1691-1699 ◽  
Author(s):  
Luigi Vitagliano ◽  
Salvatore Adinolfi ◽  
Antonio Riccio ◽  
Filomena Sica ◽  
Adriana Zagari ◽  
...  
Keyword(s):  
Domain Swapping ◽  
X Ray ◽  
Substrate Analog ◽  
Protein X ◽  
Bovine Seminal Ribonuclease

Onconase dimerization through 3D domain swapping: structural investigations and increase in the apoptotic effect in cancer cells*

2017 ◽  
Vol 474 (22) ◽  
pp. 3767-3781 ◽  
Author(s):  
Andrea Fagagnini ◽  
Andrea Pica ◽  
Sabrina Fasoli ◽  
Riccardo Montioli ◽  
Massimo Donadelli ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Three Dimensional ◽  
Homology Model ◽  
Rnase A ◽  
Domain Swapping ◽  
Structural Investigations ◽  
C Terminus ◽  
Apoptotic Effect ◽  
Bovine Seminal Ribonuclease ◽  
High Cytotoxic Activity

Onconase® (ONC), a protein extracted from the oocytes of the Rana pipiens frog, is a monomeric member of the secretory ‘pancreatic-type’ RNase superfamily. Interestingly, ONC is the only monomeric ribonuclease endowed with a high cytotoxic activity. In contrast with other monomeric RNases, ONC displays a high cytotoxic activity. In this work, we found that ONC spontaneously forms dimeric traces and that the dimer amount increases about four times after lyophilization from acetic acid solutions. Differently from RNase A (bovine pancreatic ribonuclease) and the bovine seminal ribonuclease, which produce N- and C-terminal domain-swapped conformers, ONC forms only one dimer, here named ONC-D. Cross-linking with divinylsulfone reveals that this dimer forms through the three-dimensional domain swapping of its N-termini, being the C-terminus blocked by a disulfide bond. Also, a homology model is proposed for ONC-D, starting from the well-known structure of RNase A N-swapped dimer and taking into account the results obtained from spectroscopic and stability analyses. Finally, we show that ONC is more cytotoxic and exerts a higher apoptotic effect in its dimeric rather than in its monomeric form, either when administered alone or when accompanied by the chemotherapeutic drug gemcitabine. These results suggest new promising implications in cancer treatment.

A potential allosteric subsite generated by domain swapping in bovine seminal ribonuclease 1 1Edited by A. R. Fersht

1999 ◽  
Vol 293 (3) ◽  
pp. 569-577 ◽  
Author(s):  
Luigi Vitagliano ◽  
Salvatore Adinolfi ◽  
Filomena Sica ◽  
Antonello Merlino ◽  
Adriana Zagari ◽  
...  
Keyword(s):  
Domain Swapping ◽  
Bovine Seminal Ribonuclease

Protein Reconstitution and 3D Domain Swapping

2002 ◽  
Vol 3 (6) ◽  
pp. 629-642 ◽  
Author(s):  
Maria Hakansson ◽  
Sara Linse
Keyword(s):  
Domain Swapping ◽  
Protein Reconstitution

scholarly journals Interactions Crucial for Three-Dimensional Domain Swapping in the HP-RNase Variant PM8

2011 ◽  
Vol 101 (2) ◽  
pp. 459-467 ◽  
Author(s):  
Pere Tubert ◽  
Douglas V. Laurents ◽  
Marc Ribó ◽  
Marta Bruix ◽  
Maria Vilanova ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Domain Swapping ◽  
Dimensional Domain

scholarly journals 3D Domain Swapping Modulates the Stability of Members of an Icosahedral Virus Group

Structure ◽  
2000 ◽  
Vol 8 (10) ◽  
pp. 1095-1103 ◽  
Author(s):  
Chunxu Qu ◽  
Lars Liljas ◽  
Natacha Opalka ◽  
Christophe Brugidou ◽  
Mark Yeager ◽  
...  
Keyword(s):  
Domain Swapping ◽  
Virus Group ◽  
Icosahedral Virus ◽  
The Stability

Active Monomeric and Dimeric Forms ofPseudomonas putidaGlyoxalase I:  Evidence for 3D Domain Swapping†

Biochemistry ◽  
1998 ◽  
Vol 37 (29) ◽  
pp. 10345-10353 ◽  
Author(s):  
André P. Saint-Jean ◽  
Kristina R. Phillips ◽  
Donald J. Creighton ◽  
Martin J. Stone
Keyword(s):  
Domain Swapping
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