scholarly journals Detection of a latent soluble form of membrane type 1 matrix metalloprotease bound with tissue inhibitor of matrix metalloproteinases-2 in periprosthetic tissues and fluids from loose arthroplasty endoprostheses

FEBS Journal ◽  
2013 ◽  
Vol 280 (24) ◽  
pp. 6541-6555 ◽  
Author(s):  
Anna Niarakis ◽  
Eleftheria Giannopoulou ◽  
Panagiota Ravazoula ◽  
Elias Panagiotopoulos ◽  
Ioannis K. Zarkadis ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Soluble Form ◽  
Matrix Metalloprotease ◽  
Tissue Inhibitor ◽  
Membrane Type

Articular Cartilage Fragmentation Improves Chondrocyte Migration by Upregulating Membrane Type 1 Matrix Metalloprotease

Cartilage ◽  
2021 ◽  
pp. 194760352110354
Author(s):  
Yunliang Lei ◽  
Jiabin Peng ◽  
Zhu Dai ◽  
Ying Liao ◽  
Quanhui Liu ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Articular Chondrocytes ◽  
Laser Scanning Microscopy ◽  
Gelatin Sponge ◽  
Confocal Laser ◽  
Matrix Metalloprotease ◽  
Membrane Type ◽  
Chondrocyte Migration

Objective This study was undertaken to elucidate the mechanism of improved chondrocyte migration after juvenile articular cartilage fragmentation. Design In vitro organ culture with rabbit cartilage fragments and cell culture with rabbit chondrocytes were performed. In part A, minced juvenile cartilage fragments (~0.5 × 0.5 × 0.5 mm) from rabbits, planted in gelatin sponge and fibrin glue, were cultured for 2, 4, or 6 weeks in vitro and compared with the cartilage chunks (~4 × 4 × 1 mm) and membrane type 1 matrix metalloprotease (MT1-MMP) inhibitor groups. Chondrocyte outgrowth was evaluated on histology and confocal laser scanning microscopy. MT1-MMP expression was compared between the cartilage fragment group and the cartilage chunks group. In part B, articular chondrocytes were harvested from juvenile rabbits, MT1-MMP was transfected into the cells, and cell migration was evaluated using the Transwell and wound healing tests. Results The histology and confocal microscopy results revealed that cell accumulation occurred at the edge of cartilage fragments, and outgrowth was better in the cartilage fragment group than those in the cartilage chunks group. Similar results were observed for MT1-MMP expression. After MT1-MMP inhibition, cells did not accumulate at the edge of the cartilage fragments, and chondrocyte outgrowth did not occur. Furthermore, overexpression of MT1-MMP enhanced the migration of articular chondrocytes. Conclusions Juvenile articular cartilage fragmentation improved chondrocyte migration by upregulating MT1-MMP.

Prognostic Role of Matrix Metalloproteinases 2, 7, 8, 9 and Their Type 1 Tissue Inhibitor in Blood Serum of Patients with Kidney Cancer

2020 ◽  
Vol 168 (5) ◽  
pp. 673-676
Author(s):  
N. E. Kushlinskii ◽  
E. S. Gershtein ◽  
A. A. Alferov ◽  
S. D. Bezhanova ◽  
V. V. Mushtenko ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Blood Serum ◽  
Kidney Cancer ◽  
Prognostic Role ◽  
Tissue Inhibitor

scholarly journals Substrate choice of membrane-type 1 matrix metalloproteinase is dictated by tissue inhibitor of metalloproteinase-2 levels

2007 ◽  
Vol 98 (4) ◽  
pp. 563-568 ◽  
Author(s):  
Tomoya Kudo ◽  
Takahisa Takino ◽  
Hisashi Miyamori ◽  
Erik W. Thompson ◽  
Hiroshi Sato
Keyword(s):  
Matrix Metalloproteinase ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Tissue Inhibitor ◽  
Substrate Choice ◽  
Membrane Type
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