scholarly journals The silicatein propeptide acts as inhibitor/modulator of self-organization during spicule axial filament formation

FEBS Journal ◽  
2013 ◽  
Vol 280 (7) ◽  
pp. 1693-1708 ◽  
Author(s):  
Werner E. G. Müller ◽  
Heinz C. Schröder ◽  
Sandra Muth ◽  
Sabine Gietzen ◽  
Michael Korzhev ◽  
...  
2021 ◽  
Author(s):  
David M Roberts ◽  
Anna Anchimiuk ◽  
Tomas G Kloosterman ◽  
Heath Murray ◽  
Ling Juan Wu ◽  
...  

SMC complexes, loaded at ParB-parS sites, are key mediators of chromosome organization in bacteria. ParA/Soj proteins interact with ParB/Spo0J in a pathway involving ATP-dependent dimerization and DNA binding, leading to chromosome segregation and SMC loading. In Bacillus subtilis, ParA/Soj also regulates DNA replication initiation, and along with ParB/Spo0J is involved in cell cycle changes during endospore formation. The first morphological stage in sporulation is the formation of an elongated chromosome structure called an axial filament. We now show that a major redistribution of SMC complexes drives axial filament formation, in a process regulated by ParA/Soj. Unexpectedly, this regulation is dependent on monomeric forms of ParA/Soj that cannot bind DNA or hydrolyse ATP. These results reveal a new role for ParA/Soj proteins in the regulation of SMC dynamics in bacteria, and yet further complexity in the web of interactions involving chromosome replication, segregation, and organization, controlled by ParAB and SMC.


Author(s):  
Ezzatollah Keyhani ◽  
Larry F. Lemanski ◽  
Sharon L. Lemanski

Energy for sperm motility is provided by both glycolytic and respiratory pathways. Mitochondria are involved in the latter pathway and conserve energy of substrate oxidation by coupling to phosphorylation. During spermatogenesis, the mitochondria undergo extensive transformation which in many species leads to the formation of a nebemkem. The nebemkem subsequently forms into a helix around the axial filament complex in the middle piece of spermatozoa.Immature spermatozoa of axolotls contain numerous small spherical mitochondria which are randomly distributed throughout the cytoplasm (Fig. 1). As maturation progresses, the mitochondria appear to migrate to the middle piece region where they become tightly packed to form a crystalline-like sheath. The cytoplasm in this region is no longer abundant (Fig. 2) and the plasma membrane is now closely apposed to the outside of the mitochondrial layer.


1994 ◽  
Vol 39 (9) ◽  
pp. 916-916
Author(s):  
Terri Gullickson

1996 ◽  
Author(s):  
Z. Kupper ◽  
W. Tschacher ◽  
H. Hoffmann
Keyword(s):  

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