Zizyphin modulates calcium signalling in human taste bud cells and fat taste perception in the mouse

2017 ◽  
Vol 31 (5) ◽  
pp. 486-494 ◽  
Author(s):  
Babar Murtaza ◽  
Meryem Berrichi ◽  
Chahid Bennamar ◽  
Thierry Tordjmann ◽  
Fatima Z. Djeziri ◽  
...  
Keyword(s):  
Calcium Signalling ◽  
Taste Perception ◽  
Taste Bud ◽  
Fat Taste ◽  
Taste Bud Cells

scholarly journals Orosensory Detection of Dietary Fatty Acids Is Altered in CB1R−/− Mice

Nutrients ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1347 ◽  
Author(s):  
Léa Brissard ◽  
Julia Leemput ◽  
Aziz Hichami ◽  
Patricia Passilly-Degrace ◽  
Guillaume Maquart ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Endocannabinoid System ◽  
Dietary Fatty Acids ◽  
Taste Perception ◽  
Taste Bud ◽  
Health Issues ◽  
Long Chain Fatty Acid ◽  
Protein Expressions ◽  
Fat Taste ◽  
Taste Bud Cells

Obesity is one of the major public health issues, and its prevalence is steadily increasing all the world over. The endocannabinoid system (ECS) has been shown to be involved in the intake of palatable food via activation of cannabinoid 1 receptor (CB1R). However, the involvement of lingual CB1R in the orosensory perception of dietary fatty acids has never been investigated. In the present study, behavioral tests on CB1R−/− and wild type (WT) mice showed that the invalidation of Cb1r gene was associated with low preference for solutions containing rapeseed oil or a long-chain fatty acid (LCFA), such as linoleic acid (LA). Administration of rimonabant, a CB1R inverse agonist, in mice also brought about a low preference for dietary fat. No difference in CD36 and GPR120 protein expressions were observed in taste bud cells (TBC) from WT and CB1R−/− mice. However, LCFA induced a higher increase in [Ca2+]i in TBC from WT mice than that in TBC from CB1R−/− mice. TBC from CB1R−/− mice also exhibited decreased Proglucagon and Glp-1r mRNA and a low GLP-1 basal level. We report that CB1R is involved in fat taste perception via calcium signaling and GLP-1 secretion.

scholarly journals Does intervention with GLP-1 receptor agonist semaglutide modulate perception of sweet taste in women with obesity: study protocol of a randomized, single-blinded, placebo-controlled clinical trial

Trials ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Mojca Jensterle ◽  
Simona Ferjan ◽  
Tadej Battelino ◽  
Jernej Kovač ◽  
Saba Battelino ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Neural Response ◽  
Taste Perception ◽  
Taste Bud ◽  
Controlled Clinical Trial ◽  
Food Cues ◽  
Link Type ◽  
Visual Food Cues ◽  
Taste Bud Cells

Abstract Background Preclinical studies demonstrated that glucagon-like peptide 1 (GLP-1) is locally synthesized in taste bud cells and that GLP-1 receptor exists on the gustatory nerves in close proximity to GLP-1-containing taste bud cells. This local paracrine GLP-1 signalling seems to be specifically involved in the perception of sweets. However, the role of GLP-1 in taste perception remains largely unaddressed in clinical studies. Whether any weight-reducing effects of GLP-1 receptor agonists are mediated through the modulation of taste perception is currently unknown. Methods and analysis This is an investigator-initiated, randomized single-blind, placebo-controlled clinical trial. We will enrol 30 women with obesity and polycystic ovary syndrome (PCOS). Participants will be randomized in a 1:1 ratio to either semaglutide 1.0 mg or placebo for 16 weeks. The primary endpoints are alteration of transcriptomic profile of tongue tissue as changes in expression level from baseline to follow-up after 16 weeks of treatment, measured by RNA sequencing, and change in taste sensitivity as detected by chemical gustometry. Secondary endpoints include change in neural response to visual food cues and to sweet-tasting substances as assessed by functional MRI, change in body weight, change in fat mass and change in eating behaviour and food intake. Discussion This is the first study to investigate the role of semaglutide on taste perception, along with a neural response to visual food cues in reward processing regions. The study may identify the tongue and the taste perception as a novel target for GLP-1 receptor agonists. Ethics and disseminations The study has been approved by the Slovene National Medical Ethics Committee and will be conducted in accordance with the Declaration of Helsinki and Good Clinical Practice guidelines. Results will be submitted for publication in an international peer-reviewed scientific journal. Trial registration ClinicalTrials.govNCT04263415. Retrospectively registered on 10 February 2020

scholarly journals Novel GPR120 agonist TUG891 modulates fat taste perception and preference and activates tongue-brain-gut axis in mice

2019 ◽  
Vol 61 (2) ◽  
pp. 133-142 ◽  
Author(s):  
Babar Murtaza ◽  
Aziz Hichami ◽  
Amira S. Khan ◽  
Bharat Shimpukade ◽  
Trond Ulven ◽  
...  
Keyword(s):  
Fatty Acid ◽  
In Vitro Release ◽  
Dietary Fatty Acids ◽  
Taste Perception ◽  
Taste Bud ◽  
Fat Taste ◽  
Glucagon Like Peptide 1 ◽  
Fat Preference ◽  
Human And Mouse

GPR120 is implicated as a lipid receptor in the oro-sensory detection of dietary fatty acids. However, the effects of GPR120 activation on dietary fat intake or obesity are not clearly understood. We investigated to determine whether the binding of TUG891, a novel GPR120 agonist, to lingual GPR120 modulates fat preference in mice. We explored the effects of TUG891 on obesity-related hormones and conducted behavioral choice tests on mice to better understand the physiologic relevance of the action of TUG891. In cultured mouse and human taste bud cells (TBCs), TUG891 induced a rapid increase in Ca2+ by acting on GPR120. A long-chain dietary fatty acid, linoleic acid (LA), also recruited Ca2+ via GPR120 in human and mouse TBCs. Both TUG891 and LA induced ERK1/2 phosphorylation and enhanced in vitro release of glucagon-like peptide-1 from cultured human and mouse TBCs. In situ application of TUG891 onto the tongue of anesthetized mice triggered the secretion of pancreatobiliary juice, probably via the tongue-brain-gut axis. Furthermore, lingual application of TUG891 altered circulating concentrations of cholecystokinin and adipokines, associated with decreased circulating LDL, in conscious mice. In behavioral tests, mice exhibited a spontaneous preference for solutions containing either TUG891 or LA instead of a control. However, addition of TUG891 to a solution containing LA significantly curtailed fatty acid preference. Our study demonstrates that TUG891 binds to lingual GPR120 receptors, activates the tongue-brain-gut axis, and modulates fat preference. These findings may support the development of new fat taste analogs that can change the approach to obesity prevention and treatment.

Some Observations on Changes in Denervated Taste Buds of Circumvallate Papillae of Rabbits

1969 ◽  
Vol 27 ◽  
pp. 308-309
Author(s):  
Sunao Fujimoto ◽  
Raymond G. Murray ◽  
Assia Murray
Keyword(s):  
Taste Buds ◽  
Taste Bud ◽  
Cell Type ◽  
Dark Cells ◽  
Type Iii ◽  
Circumvallate Papillae ◽  
Taste Bud Cells ◽  
Light Cells

Taste bud cells in circumvallate papillae of rabbit have been classified into three groups: dark cells; light cells; and type III cells. Unilateral section of the 9th nerve distal to the petrosal ganglion was performed in 18 animals, and changes of each cell type in the denervated buds were observed from 6 hours to 10 days after the operation.Degeneration of nerves is evident at 12 hours (Fig. 1) and by 2 days, nerves are completely lacking in the buds. Invasion by leucocytes into the buds is remarkable from 6 to 12 hours but then decreases. Their extrusion through the pore is seen. Shrinkage and disturbance in arrangement of cells in the buds can be seen at 2 days. Degenerated buds consisting of a few irregular cells and remnants of degenerated cells are present at 4 days, but buds apparently normal except for the loss of nerve elements are still present at 6 days.

scholarly journals Mechanism of fat taste perception: Association with diet and obesity

2016 ◽  
Vol 63 ◽  
pp. 41-49 ◽  
Author(s):  
Dongli Liu ◽  
Nicholas Archer ◽  
Konsta Duesing ◽  
Garry Hannan ◽  
Russell Keast
Keyword(s):  
Taste Perception ◽  
Fat Taste

Voltage-gated currents of taste bud cells in infant mice

2007 ◽  
Vol 58 ◽  
pp. S217
Author(s):  
Masafumi Iwamoto ◽  
Yoshitaka Ohtubo ◽  
Kiyonori Yoshii
Keyword(s):  
Taste Bud ◽  
Voltage Gated ◽  
Taste Bud Cells

scholarly journals Fatty Acid Lingual Application Activates Gustatory and Reward Brain Circuits in the Mouse

Nutrients ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 1246 ◽  
Author(s):  
Yvan Peterschmitt ◽  
Souleymane Abdoul-Azize ◽  
Babar Murtaza ◽  
Marie Barbier ◽  
Amira Khan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Dietary Lipids ◽  
Taste Perception ◽  
Neuronal Activation ◽  
Glut 1 ◽  
Brain Circuits ◽  
Nucleus Of Solitary Tract ◽  
Fat Taste ◽  
Fos Expression ◽  
Reward Pathways

The origin of spontaneous preference for dietary lipids in humans and rodents is debated, though recent compelling evidence has shown the existence of fat taste that might be considered a sixth taste quality. We investigated the implication of gustatory and reward brain circuits, triggered by linoleic acid (LA), a long-chain fatty acid. The LA was applied onto the circumvallate papillae for 30 min in conscious C57BL/6J mice, and neuronal activation was assessed using c-Fos immunohistochemistry. By using real-time reverse transcription polymerase chain reaction (RT-qPCR), we also studied the expression of mRNA encoding brain-derived neurotrophic factor (BDNF), Zif-268, and Glut-1 in some brain areas of these animals. LA induced a significant increase in c-Fos expression in the nucleus of solitary tract (NST), parabrachial nucleus (PBN), and ventroposterior medialis parvocellularis (VPMPC) of the thalamus, which are the regions known to be activated by gustatory signals. LA also triggered c-Fos expression in the central amygdala and ventral tegmental area (VTA), involved in food reward, in conjunction with emotional traits. Interestingly, we noticed a high expression of BDNF, Zif-268, and Glut-1 mRNA in the arcuate nucleus (Arc) and hippocampus (Hipp), where neuronal activation leads to memory formation. Our study demonstrates that oral lipid taste perception might trigger the activation of canonical gustatory and reward pathways.

Expression of Neurotrophic Factors and Their Receptors in Rat Posterior Taste Bud Cells

2014 ◽  
Vol 39 (2) ◽  
pp. 107-114
Author(s):  
Dong-Il Park ◽  
◽  
Ki-Myung Chung ◽  
Young-Kyung Cho ◽  
Kyung-Nyun Kim
Keyword(s):  
Neurotrophic Factors ◽  
Taste Bud ◽  
Taste Bud Cells
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