scholarly journals Preliminary observations of a new approach to tissue repair: Peripheral blood mononuclear cells in platelet-rich plasma injected into skin graft area

2018 ◽  
Vol 27 (7) ◽  
pp. 795-797 ◽  
Author(s):  
Catuscia Orlandi ◽  
Elena Bondioli ◽  
Michela Venturi ◽  
Davide Melandri
Keyword(s):  
Skin Graft ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Tissue Repair ◽  
Mononuclear Cells ◽  
Platelet Rich Plasma ◽  
Peripheral Blood Mononuclear ◽  
New Approach ◽  
Blood Mononuclear Cells ◽  
Graft Area

Beta-Endorphin Concentrations in the Peripheral Blood Mononuclear Cells of Migraine and Tension-Type Headache Patients

Cephalalgia ◽  
1992 ◽  
Vol 12 (3) ◽  
pp. 155-157 ◽  
Author(s):  
Massimo Leone ◽  
Paola Sacerdote ◽  
Domenico D'Amico ◽  
Alberto E Panerai ◽  
Gennaro Bussone
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Prophylactic Treatment ◽  
Mononuclear Cells ◽  
Tension Type Headache ◽  
Peripheral Blood Mononuclear ◽  
New Approach ◽  
Blood Mononuclear Cells ◽  
Type Headache ◽  
Headache Patients

Levels of b-endorphin in peripheral blood mononuclear cells have been studied as a new approach to investigating opioid tone in migraine and tension-type headache. Sixty-one patients with migraine without aura, 39 with migraine with aura and 23 with episodic tension-type headache were compared with 37 healthy controls. Peripheral blood samples were taken from patients not enduring headache attacks and not undergoing prophylactic treatment. A significant reduction in peripheral blood mononuclear cell b-endorphin concentrations was observed in migraine patients with and without aura, but not in tension-type headache patients. Altered transmitter modulation to peripheral blood mononuclear cells may be the cause of this alteration, which could be part of a more diffuse opioid system derangement in migraine subjects.

G-CSF-mobilized peripheral blood mononuclear cells combined with platelet-rich plasma accelerate restoration of ovarian function in cyclophosphamide-induced POI rats†

2019 ◽  
Vol 101 (1) ◽  
pp. 91-101 ◽  
Author(s):  
Qiuyan Huang ◽  
Bo Liu ◽  
Rufang Jiang ◽  
Shengbin Liao ◽  
Zhiyao Wei ◽  
...  
Keyword(s):  
Granulosa Cell ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Cell Apoptosis ◽  
Mononuclear Cells ◽  
Ovarian Function ◽  
Platelet Rich Plasma ◽  
Female Rats ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Abstract Peripheral blood mononuclear cells (PBMCs) are rich in hematopoietic cells and mesenchymal stem cells. Platelet-rich plasma (PRP) is rich in various growth factors. PBMCs and PRP have been suggested, individually, to restore ovarian function by improving the local microenvironment. The current study investigated the effect of granulocyte colony-stimulating factor (G-CSF)-mobilized PBMCs combined with PRP on restoring ovarian function in rats with primary ovarian insufficiency (POI). Thirty adult female rats were randomly subdivided into five groups: normal control (control), cyclophosphamide (CTX) plus subsequent PBS (POI + PBS), CTX plus subsequent PRP (POI + PRP), CTX plus subsequent G-CSF-mobilized PBMCs (POI + PBMCs), and CTX plus subsequent G-CSF-mobilized PBMCs combined with PRP (POI + PBMCs + PRP). CTX exposure induced the typical POI phenotype with increased diestrus; shortened estrus; follicle arrest at all stages; decreased serum levels of estradiol-17β (E2) and anti-Mullerian hormone (AMH); and increased levels of follicle-stimulating hormone (FSH). Transplantation of mobilized PBMCs with PRP resulted in a much earlier restoration of the estrous cycle, sex hormone levels, and preantral follicle growth in POI rats. Expression of the male-specific Sry gene in the ovarian tissues of POI + PBMCs + PRP female recipient rats was evident at 5, 10, and 20 days posttransplantation along with significant increases in the expression of angiogenesis markers CD34+ and VEGF and folliculogenesis markers AMH and FSHR. Additionally, PBMCs in combination with PRP mitigated granulosa cell apoptosis by downregulating BAX and upregulating BCL-2. These results demonstrate that G-CSF-mobilized PBMCs combined with PRP accelerate the restoration of ovarian function in POI rats by increasing ovarian neovascularization, reducing granulosa cell apoptosis, and promoting folliculogenesis.

Sign in / Sign up

Export Citation Format

Share Document