scholarly journals High-glucose-cultivated peripheral blood mononuclear cells impaired keratinocyte function via reduced IL-22 expression: implications on impaired diabetic wound healing

2015 ◽  
Vol 24 (8) ◽  
pp. 639-641 ◽  
Author(s):  
Shu-Mei Huang ◽  
Ching-Shuang Wu ◽  
David Chao ◽  
Chin-Han Wu ◽  
Ching-Chia Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Peripheral Blood Mononuclear Cells ◽  
High Glucose ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Diabetic Wound ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Diabetic Wound Healing

scholarly journals miR-19a and miR-20a and Tissue Factor Expression in Activated Human Peripheral Blood Mononuclear Cells

Thrombosis ◽  
2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Cristina Balia ◽  
Mirella Giordano ◽  
Valentina Scalise ◽  
Tommaso Neri ◽  
Gabriella Fontanini ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Peripheral Blood Mononuclear Cells ◽  
High Glucose ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Inflammatory Stimuli

Background and Aims. To investigate the behaviour of miR-19a and miR-20a, two microRNAs involved in posttranscriptional modulation of TF expression in peripheral blood mononuclear cells (PBMCs) exposed to high glucose (HG) and lipopolysaccharide (LPS), and to evaluate the involvement of angiotensin II in that process. Methods. TF Procoagulant Activity (PCA, one-stage clotting assay), antigen (Ag, ELISA), and miR-19a and miR-20a levels (specific TaqMan® MicroRNA Assays) were evaluated in PBMCs exposed to high glucose (HG, 50 mM), LPS (100 ng/mL), and Olmesartan (OLM, 10−6 M), an angiotensin II type 1 receptor antagonist. Results. HG increased TF expression and decreased both miRs as compared to control glucose conditions (11.1 mM). In HG-activated PBMCs, LPS stimulated TF expression and downregulated miR-20a, an effect reverted by OLM (10−6 M); miR-19a expression was unchanged by LPS in both CG and HG conditions. Conclusions. miR-19a and miR-20a are inhibited by inflammatory stimuli active on TF expression and their response differs by the stimulus under investigation; angiotensin II may participate in that mechanism.

scholarly journals Human peripheral blood mononuclear cells enriched in endothelial progenitor cells via quality and quantity controlled culture accelerate vascularization and wound healing in a porcine wound model

2018 ◽  
Vol 27 (7) ◽  
pp. 1068-1079 ◽  
Author(s):  
Makiko Kado ◽  
Rica Tanaka ◽  
Kayo Arita ◽  
Kayoko Okada ◽  
Rie Ito-Hirano ◽  
...  
Keyword(s):  
Wound Healing ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Skin Defect ◽  
Endothelial Progenitor ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

The transplantation of endothelial progenitor cells (EPCs) is used to promote wound angiogenesis. In patients with chronic wounds and accompanying morbidities, EPCs are often compromised in number and function. To overcome these limitations, we previously developed a quality and quantity controlled (QQ) culture system to enrich peripheral blood mononuclear cells (PBMNCs) in EPCs. To evaluate the wound healing efficacy of mononuclear cells (MNCs) harvested after QQ culture (QQMNCs), preclinical studies were performed on large animals. MNCs harvested from the blood of healthy human subjects were cultured in the presence of angiogenic cytokines and growth factors in a serum-free medium for 7 days. A total of 5 × 106 QQMNCs per full-thickness skin defect or control saline was injected into wounds induced in cyclosporine-immunosuppressed pigs. EPC colony-forming assays revealed a significantly higher number of definitive (partially differentiated) EPC colony-forming units in QQMNCs. Flow cytometry evaluation of QQMNC surface markers showed enrichment of CD34+ and CD133+ stem cell populations, significant reduction in CCR2+ cell percentages, and a greater than 10-fold increase in the percentage of anti-inflammatory M2-type macrophages (CD206+ cells) compared with PBMNCs. Wounds treated with QQMNCs had a significantly higher closure rate. Wounds were harvested, frozen, and sectioned at day 21 postoperatively. Hematoxylin and eosin staining revealed that the epithelization of QQMNC-treated wounds was more advanced than in controls. Treated wounds developed granulation tissue with more mature collagen and larger capillary networks. CD31 and human mitochondrial co-staining confirmed the presence of differentiated human cells within newly formed vessels. Real-time polymerase chain reaction (PCR) showed upregulation of interleukin 6 (IL-6), IL-10, and IL-4 in the wound bed, suggesting paracrine activity of the transplanted QQMNCs. Our data demonstrate for the first time that QQ culture of MNCs obtained from a small amount of peripheral blood yields vasculogenic and therapeutic cells effective in wound healing.

scholarly journals Secretome of Peripheral Blood Mononuclear Cells Enhances Wound Healing

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e60103 ◽  
Author(s):  
Michael Mildner ◽  
Stefan Hacker ◽  
Thomas Haider ◽  
Maria Gschwandtner ◽  
Gregor Werba ◽  
...  
Keyword(s):  
Wound Healing ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Regulatory Effects of High Glucose on the Expression of Toll-Like Receptor Gene and Cytokine Production in Human Peripheral-Blood Mononuclear Cells

2011 ◽  
Vol 179-180 ◽  
pp. 374-379 ◽  
Author(s):  
Jing Sheng Lan ◽  
Yu Kang Dong ◽  
Xing Ming Cai
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
High Glucose ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Toll Like Receptor ◽  
Peripheral Blood Mononuclear ◽  
Tnf Α ◽  
Blood Mononuclear Cells ◽  
Regulatory Effects

To investigate the regulatory effects of high glucose on the expression of Toll Like Receptor(TLR) Gene and the level of tumor necrosis factor(TNF)-α and interleukin(IL) -6 in human peripheral-blood mononuclear cells . Methods The alterations of TLR1～10 mRNA expression in human peripheral-blood mononuclear cells were quantitated using real-time quantitative-polymerasechain reaction. The level of TNF-α and IL-6 were measured by Enzyme-Linked Immuno Sorbent Assay . The anti-TLRmAb were used to block the mononuclear cells 30 min, high glucose was used to stimulate the cells. Rusults high glucose strongly up-regulated the expression of TLR3、5 mRNA but the expression of the other sub-TLRs weren’t changed .The concentrations of TNF-α、IL-6 were 86.40±8.46 and 874.66±92.84 in high glucose group, all significantly higher than that the control experiment (60.49±6.80 and 541.80±81.08 , all P<0.01), The anti-TLR3mAb and anti-TLR5mAb were used to block the mononuclear cells , The concentrations of TNF-α、IL-6 (72.41±8.52 and 700.59±84.88) were lower than that in the high glucose group, all P<0.05 . Conclusion high glucose may be endogenous ligand of TLRs and high glucose can regulate the release of inflammation cytokines from human peripheral-blood mononuclear cells through TLR signal way.

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