scholarly journals Readthrough of stop codons by use of aminoglycosides in cells from xeroderma pigmentosum group C patients

2015 ◽  
Vol 24 (4) ◽  
pp. 296-297 ◽  
Author(s):  
Christiane Kuschal ◽  
Sikandar G. Khan ◽  
Benedikt Enk ◽  
John J. DiGiovanna ◽  
Kenneth H. Kraemer
Keyword(s):  
Xeroderma Pigmentosum ◽  
Stop Codons ◽  
In Cells

scholarly journals 40S ribosome profiling reveals distinct roles for Tma20/Tma22 (MCT-1/DENR) and Tma64 (eIF2D) in 40S subunit recycling

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
David J. Young ◽  
Sezen Meydan ◽  
Nicholas R. Guydosh
Keyword(s):  
Protein Synthesis ◽  
Neurological Disease ◽  
Ribosome Profiling ◽  
Minor Role ◽  
Stop Codons ◽  
Genes Encoding ◽  
Ribosome Footprinting ◽  
A Minor ◽  
And Control ◽  
In Cells

AbstractThe recycling of ribosomes at stop codons for use in further rounds of translation is critical for efficient protein synthesis. Removal of the 60S subunit is catalyzed by the ATPase Rli1 (ABCE1) while removal of the 40S is thought to require Tma64 (eIF2D), Tma20 (MCT-1), and Tma22 (DENR). However, it remains unclear how these Tma proteins cause 40S removal and control reinitiation of downstream translation. Here we used a 40S ribosome footprinting strategy to directly observe intermediate steps of ribosome recycling in cells. Deletion of the genes encoding these Tma proteins resulted in broad accumulation of unrecycled 40S subunits at stop codons, directly establishing their role in 40S recycling. Furthermore, the Tma20/Tma22 heterodimer was responsible for a majority of 40S recycling events while Tma64 played a minor role. Introduction of an autism-associated mutation into TMA22 resulted in a loss of 40S recycling activity, linking ribosome recycling and neurological disease.

RNA dependent DNA polymerase in cells of xeroderma pigmentosum

1971 ◽  
Vol 44 (2) ◽  
pp. 433-438 ◽  
Author(s):  
Werner E.G. Müller ◽  
Zen-Ichi Yamazaki ◽  
Rudolf K. Zahn ◽  
Georg Brehm ◽  
Günter Korting
Keyword(s):  
Dna Polymerase ◽  
Xeroderma Pigmentosum ◽  
In Cells

UV-induced DNA repair synthesis in cells of patients with different forms of xeroderma pigmentosum and of heterozygotes

1973 ◽  
Vol 20 (3) ◽  
pp. 417-428 ◽  
Author(s):  
W.J. Kleijer ◽  
E.A. De Weerd-Kastelein ◽  
M.L. Sluyter ◽  
W. Keijzer ◽  
J. De Wit ◽  
...  
Keyword(s):  
Dna Repair ◽  
Xeroderma Pigmentosum ◽  
Repair Synthesis ◽  
Dna Repair Synthesis ◽  
In Cells
Sign in / Sign up

Export Citation Format

Share Document