Penetration and biological effects of topically applied cyclosporin A nanoparticles in a human skin organ culture inflammatory model

2012 ◽  
Vol 21 (12) ◽  
pp. 938-943 ◽  
Author(s):  
Marina Frušić-Zlotkin ◽  
Yoram Soroka ◽  
Ran Tivony ◽  
Liraz Larush ◽  
Lilian Verkhovsky ◽  
...  
Keyword(s):  
Organ Culture ◽  
Cyclosporin A ◽  
Human Skin ◽  
Biological Effects ◽  
Skin Organ Culture

scholarly journals Kinetic Cytokine Secretion Profile of LPS-Induced Inflammation in the Human Skin Organ Culture

Pharmaceutics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 299 ◽  
Author(s):  
Raanan Gvirtz ◽  
Navit Ogen-Shtern ◽  
Guy Cohen
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Comprehensive Evaluation ◽  
Ex Vivo ◽  
Cytokine Secretion ◽  
Peak Time ◽  
Local Skin ◽  
The Impact ◽  
Skin Organ Culture

Several in vitro models that mimic different aspects of local skin inflammation exist. The use of ex vivo human skin organ culture (HSOC) has been reported previously. However, comprehensive evaluation of the cytokine secretory capacity of the system and its kinetics has not been performed. Objective: the aim of the current study was to investigate the levels and secretion pattern of key cytokine from human skin tissue upon lipopolysaccharide (LPS) stimulation. HSOC maintained in an air–liquid interface was used. Epidermal and tissue viability was monitored by MTT and Lactate Dehydrogenase (LDH) activity assay, respectively. Cytokine levels were examined by ELISA and multiplex array. HSOCs were treated without or with three different LPS subtypes and the impact on IL-6 and IL-8 secretion was evaluated. The compounds enhanced the secreted levels of both cytokines. However, differences were observed in their efficacy and potency. Next, a kinetic multiplex analysis was performed on LPS-stimulated explants taken from three different donors to evaluate the cytokine secretion pattern during 0–72 h post-induction. The results revealed that the pro-inflammatory cytokines IL-6, IL-8, TNFα and IL-1β were up-regulated by LPS stimuli. IL-10, an anti-inflammatory cytokine, was also induced by LPS, but exhibited a different secretion pattern, peak time and maximal stimulation values. IL-1α and IL-15 showed donor-specific changes. Lastly, dexamethasone attenuated cytokine secretion in five independent repetitions, supporting the ability of the system to be used for drug screening. The collective results demonstrate that several cytokines can be used as valid inflammatory markers, regardless of changes in the secretion levels due to donor’s specific alterations.

Translational Use of a Standardized Full Human Skin Organ Culture Model in Autoimmune Blistering Diseases

2019 ◽  
Vol 85 (1) ◽  
pp. e56
Author(s):  
Imke A. K. Burmester ◽  
Shirin Emtenani ◽  
Jan-Gerrit Johns ◽  
Ralf J. Ludwig ◽  
Christoph M. Hammers ◽  
...  
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Culture Model ◽  
Skin Organ Culture ◽  
Autoimmune Blistering Diseases

Evaluation of topically applied copper(II) oxide nanoparticle cytotoxicity in human skin organ culture

2013 ◽  
Vol 27 (1) ◽  
pp. 292-298 ◽  
Author(s):  
Dror Cohen ◽  
Yoram Soroka ◽  
Zeev Ma’or ◽  
Miriam Oron ◽  
Meital Portugal-Cohen ◽  
...  
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Oxide Nanoparticle ◽  
Skin Organ Culture

Human Skin Organ Culture

Wound Healing ◽  
2003 ◽  
pp. 305-310 ◽  
Author(s):  
Ingrid Moll
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Skin Organ Culture

scholarly journals 086 Human skin organ culture as a clinically relevant model for studying VEGF-dependent angiogenesis ex vivo

2019 ◽  
Vol 139 (9) ◽  
pp. S229
Author(s):  
A. Luengas-Martinez ◽  
J. Hardman ◽  
D. Rutkowski ◽  
R. Paus ◽  
H. Young
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Ex Vivo ◽  
Relevant Model ◽  
Skin Organ Culture

scholarly journals Anti-inflammatory and antiradical effects of a 2% diosmin cream in a human skin organ culture as model

2018 ◽  
Vol 17 (5) ◽  
pp. 848-854 ◽  
Author(s):  
Sylvie Boisnic ◽  
Marie-Christine Branchet ◽  
Christelle Gouhier-Kodas ◽  
François Verriere ◽  
Violaine Jabbour
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Anti Inflammatory ◽  
Skin Organ Culture
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