scholarly journals Equid alphaherpesviruses 8 and 9: strain isolation, ORF30 sequencing and antiviral assay

2021 ◽  
Vol 53 (S56) ◽  
pp. 62-63
Keyword(s):  
Strain Isolation

scholarly journals Efficient Bioelectrochemical Conversion of Industrial Wastewater by Specific Strain Isolation and Community Adaptation

2019 ◽  
Vol 7 ◽  
Author(s):  
Stefanie Brunner ◽  
Tina Klessing ◽  
Andreas Dötsch ◽  
Katrin Sturm-Richter ◽  
Johannes Gescher
Keyword(s):  
Industrial Wastewater ◽  
Community Adaptation ◽  
Strain Isolation ◽  
Specific Strain

scholarly journals The evolution of hard tick-borne relapsing fever borreliae is correlated with vector species rather than geographical distance

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ranna Nakao ◽  
Kentaro Kasama ◽  
Bazartseren Boldbaatar ◽  
Yoshitoshi Ogura ◽  
Hiroki Kawabata ◽  
...  
Keyword(s):  
Core Gene ◽  
Genetic Distances ◽  
Hard Tick ◽  
Vector Species ◽  
Relapsing Fever ◽  
Strain Isolation ◽  
Ecological Features ◽  
Geographical Distances ◽  
History Of ◽  
Hokkaido Island

Abstract Background Relapsing fever (RF) borreliae are arthropod-borne spirochetes and some of them cause human diseases, which are characterized by relapsing or recurring episodes of fever. Recently, it has been classified into two groups: soft tick-borne RF (STRF) borreliae and hard tick-borne RF (HTRF) borreliae. STRF borreliae include classical RF agents and HTRF borreliae, the latter of which include B. miyamotoi, a human pathogen recently identified in Eurasia and North America. Results In this study, we determined the genome sequences of 16 HTRF borreliae strains: 15 B. miyamotoi strains (9 from Hokkaido Island, Japan, 3 from Honshu Island, Japan, and 3 from Mongolia) and a Borrelia sp. tHM16w. Chromosomal gene synteny was highly conserved among the HTRF strains sequenced in this study, even though they were isolated from different geographic regions and different tick species. Phylogenetic analysis based on core gene sequences revealed that HTRF and STRF borreliae are clearly distinguishable, with each forming a monophyletic group in the RF borreliae lineage. Moreover, the evolutionary relationships of RF borreliae are consistent with the biological and ecological features of each RF borreliae sublineage and can explain the unique characteristics of Borrelia anserina. In addition, the pairwise genetic distances between HTRF borreliae strains were well correlated with those of vector species rather than with the geographical distances between strain isolation sites. This result suggests that the genetic diversification of HTRF borreliae is attributed to the speciation of vector ticks and that this relationship might be required for efficient transmission of HTRF borreliae within vector ticks. Conclusions The results of the present study, together with those from previous investigations, support the hypothesis that the common ancestor of borreliae was transmitted by hard-bodied ticks and that only STRF borreliae switched to using soft-bodied ticks as a vector, which was followed by the emergence of Borrelia recurrentis, lice-borne RF borreliae. Our study clarifies the phylogenetic relationships between RF borreliae, and the data obtained will contribute to a better understanding of the evolutionary history of RF borreliae.

Microalgae Strain Isolation, Screening, and Identification for Biofuels and High-Value Products

2016 ◽  
pp. 63-89 ◽  
Author(s):  
Peter Neofotis ◽  
Andy Huang ◽  
William Chang ◽  
Floral Joseph ◽  
Juergen Polle
Keyword(s):  
Strain Isolation ◽  
Screening And Identification

Strain‐Isolation Bridge Structure to Improve Stretchability of Highly Sensitive Strain Sensors

2019 ◽  
Vol 4 (9) ◽  
pp. 1900309 ◽  
Author(s):  
Yangchengyi Liu ◽  
Hanghai Fan ◽  
Kan Li ◽  
Nie Zhao ◽  
Shangda Chen ◽  
...  
Keyword(s):  
Strain Sensors ◽  
Sensitive Strain ◽  
Bridge Structure ◽  
Strain Isolation ◽  
Highly Sensitive

scholarly journals Suppression of Rice Blast by Bacterial Strains Isolated from Cultivated Soda Saline-Sodic Soils

2020 ◽  
Vol 17 (14) ◽  
pp. 5248
Author(s):  
Yi Wei ◽  
Lanhui Li ◽  
Wenjun Hu ◽  
Huiyan Ju ◽  
Mingzhe Zhang ◽  
...  
Keyword(s):  
Biological Control ◽  
Rice Blast ◽  
Rice Fields ◽  
Bacterial Strains ◽  
Sodic Soils ◽  
Strain Isolation ◽  
16S Rna ◽  
Bacillus Tequilensis ◽  
Cold Tolerant ◽  
Stenotrophomonas Rhizophila

Rice blast caused by Magnaporthe oryzae is one of the most serious rice diseases worldwide. Biological control is gaining popularity as a promising method for the control of this disease; however, more effective microbial strains with strong adaptability in rice fields need to be identified. Here, we report for the first time the successful identification of biocontrol bacterial strains from frozen soils of the soda saline-sodic land. We isolated 82 bacterial strains from rice fields in the western Songnen Plain of China, one of the three major soda saline soils in the world. Five of the isolated strains exhibited strong inhibition to M. oryzae growth. The potential strains were identified as Bacillus safensis JLS5, Pseudomonas koreensis JLS8, Pseudomonas saponiphila JLS10, Stenotrophomonas rhizophila JLS11 and Bacillus tequilensis JLS12, respectively, by 16s RNA gene sequence analysis. The antagonistic assay and the artificial inoculation tests showed that JLS5 and JLS12 could effectively inhibit conidial germination and pathogenicity of the rice blast fungus, both preventively and curatively. The suppression of pathogenicity was further confirmed by greenhouse experiments, showing the effectiveness of JLS5 and JLS12 as a potential biological control agents of M. oryzae. The potential application of these cold-tolerant strains for rice blast control in cold regions is discussed. Our data suggest that soda saline-sodic soils are a rich source for biocontrol strain isolation.

scholarly journals Patients’ Perspectives on the Prevention and Treatment of Peritonitis in Peritoneal Dialysis: A Semi-Structured Interview Study

2016 ◽  
Vol 36 (6) ◽  
pp. 631-639 ◽  
Author(s):  
Denise J. Campbell ◽  
Jonathan C. Craig ◽  
David W. Mudge ◽  
Fiona G. Brown ◽  
Germaine Wong ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Structured Interview ◽  
Financial Strain ◽  
Structured Interviews ◽  
Internal Damage ◽  
Training Support ◽  
Strain Isolation ◽  
Life Threatening ◽  
Sharing Responsibility

Background Peritoneal dialysis (PD) is recommended for adults with residual kidney function and without significant comorbidities. However, peritonitis is a serious and common complication that is associated with hospitalization, pain, catheter loss, and death. This study aims to describe the beliefs, needs, and experiences of PD patients about peritonitis, to inform the training, support, and care of these patients. Methods Qualitative semi-structured interviews were conducted with 29 patients from 3 renal units in Australia who had previous or current experience of PD. The interviews were conducted between November 2014 and November 2015. Transcripts were analyzed thematically. Results We identified 4 themes: constant vigilance for prevention (conscious of vulnerability, sharing responsibility with family, demanding attention to detail, ambiguity of detecting infection, ineradicable inhabitation, jeopardizing PD success); invading harm (life-threatening, wreaking internal damage, debilitating pain, losing control and dignity); incapacitating lifestyle interference (financial strain, isolation and separation, exacerbating burden on family); and exasperation with hospitalization (dread of hospital admission, exposure to infection, gruelling follow-up schedule, exposure to harm). Conclusions Patients perceived that peritonitis could threaten their health, treatment modality, and lifestyle, which motivated vigilance and attention to hygiene. They felt a loss of control due to debilitating symptoms including pain and having to be hospitalized, and they were uncertain about how to monitor for signs of peritonitis. Providing patients with education about the causes and signs of peritonitis and addressing their concerns about lifestyle impact, financial impact, hospitalization, and peritonitis-related anxieties may improve treatment satisfaction and outcomes for patients requiring PD.

Fungal succession in the needle litter of a montane Picea abies forest investigated through strain isolation and molecular fingerprinting

2015 ◽  
Vol 13 ◽  
pp. 157-166 ◽  
Author(s):  
Zuzana Haňáčková ◽  
Ondřej Koukol ◽  
Martina Štursová ◽  
Miroslav Kolařík ◽  
Petr Baldrian
Keyword(s):  
Picea Abies ◽  
Molecular Fingerprinting ◽  
Fungal Succession ◽  
Strain Isolation ◽  
Needle Litter

scholarly journals Microbial survival of space vacuum and extreme ultraviolet irradiation: strain isolation and analysis during a rocket flight

2002 ◽  
Vol 215 (1) ◽  
pp. 163-168 ◽  
Author(s):  
Roya Saffary ◽  
Renu Nandakumar ◽  
Dennis Spencer ◽  
Frank T Robb ◽  
Joseph M Davila ◽  
...  
Keyword(s):  
Ultraviolet Irradiation ◽  
Extreme Ultraviolet ◽  
Rocket Flight ◽  
Strain Isolation ◽  
Microbial Survival ◽  
Space Vacuum
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