scholarly journals Seroprevalence of equine herpesvirus‐1 (EHV‐1) and herpesvirus‐4 (EHV‐4) in Moroccan horse populations

2021 ◽  
Vol 53 (S56) ◽  
pp. 59-60
2002 ◽  
Vol 83 (3) ◽  
pp. 525-531 ◽  
Author(s):  
Jin-an Huang ◽  
Nino Ficorilli ◽  
Carol A. Hartley ◽  
George P. Allen ◽  
Michael J. Studdert

Open reading frame (ORF) 71 genes of both equine herpesvirus-1 (EHV-1) and EHV-4 encode a unique glycoprotein, which has been described to vary in molecular mass from 200 to 450 kDa. Using PCR and nucleotide sequence analysis, it was shown that the ORF 71 genes of EHV-1 and EHV-4 are polymorphic due to a variable number of reiterated sequences in two regions, designated regions A and B. Region A was threonine-rich and was located near the N terminus. Region B comprised a 38 amino acid repeat near the C terminus that expanded following cell culture adaptation. Western blot analysis of viruses showed that EHV-4 gp2 was modified by glycosylation and that variation in region A resulted in the marked differences in the molecular mass of EHV-4 gp2.


Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2851
Author(s):  
Zineb EL Brini ◽  
Ouafaa Fassi Fihri ◽  
Romain Paillot ◽  
Chafiqa Lotfi ◽  
Farid Amraoui ◽  
...  

This study reports the first equine herpesvirus-1 (EHV-1) and equine herpesvirus-4 (EHV-4) seroprevalence investigation in horse populations of Morocco in 24 years. It also aims to determine antibody titers in horses vaccinated under field conditions with a monovalent EHV-1 vaccine. Blood samples were collected from 405 horses, including 163 unvaccinated and 242 vaccinated animals. They were tested using a commercial type-specific enzyme-linked immunosorbent assay (ELISA) and a virus neutralization test (VNT). Overall, 12.8% unvaccinated, and 21.8% vaccinated horses were positive for EHV-1. All samples were positive for EHV-4 when tested with the type-specific ELISA. In the vaccinated group, the VNT revealed a mean antibody titer of 1:49 for EHV-1 and 1:45 for EHV-4.


2016 ◽  
Vol 97 (3) ◽  
pp. 747-755 ◽  
Author(s):  
P. K. Vaz ◽  
J. Horsington ◽  
C. A. Hartley ◽  
G. F. Browning ◽  
N. P. Ficorilli ◽  
...  

2009 ◽  
Vol 86 (2) ◽  
pp. 339-344 ◽  
Author(s):  
Veysel S. Ataseven ◽  
Seval B. Dağalp ◽  
Murat Güzel ◽  
Zeynep Başaran ◽  
Mehmet T. Tan ◽  
...  

1996 ◽  
Vol 70 (11) ◽  
pp. 8195-8198 ◽  
Author(s):  
J E Wellington ◽  
G P Allen ◽  
A A Gooley ◽  
D N Love ◽  
N H Packer ◽  
...  

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 425
Author(s):  
Hyung-Woo Kang ◽  
Eun-Yong Lee ◽  
Kyoung-Ki Lee ◽  
Mi-Kyeong Ko ◽  
Ji-Young Park ◽  
...  

Equine herpesvirus-1 (EHV-1) is an important pathogen in horses. It affects horses worldwide and causes substantial economic losses. In this study, for the first time, we characterized EHV-1 isolates from South Korea at the molecular level. We then aimed to determine the genetic divergences of these isolates by comparing them to sequences in databases. In total, 338 horse samples were collected, and 12 EHV-1 were isolated. We performed ORF30, ORF33, ORF68, and ORF34 genetic analysis and carried out multi-locus sequence typing (MLST) of 12 isolated EHV-1. All isolated viruses were confirmed as non-neuropathogenic type, showing N752 of ORF30 and highly conserved ORF33 (99.7–100%). Isolates were unclassified using ORF68 analysis because of a 118 bp deletion in nucleotide sequence 701–818. Seven EHV-1 isolates (16Q4, 19R166-1, 19R166-6, 19/10/15-2, 19/10/15-4, 19/10/18-2, 19/10/22-1) belonged to group 1, clade 10, based on ORF34 and MLST analysis. The remaining 5 EHV-1 isolates (15Q25-1, 15D59, 16Q5, 16Q40, 18D99) belonged to group 7, clade 6, based on ORF34 and MLST analysis.


1999 ◽  
Vol 120 (1) ◽  
pp. 15-27 ◽  
Author(s):  
C. Walker ◽  
V.M. Perotti ◽  
D.N. Love ◽  
J.M. Whalley

Sign in / Sign up

Export Citation Format

Share Document