scholarly journals Advanced three‐dimensional culture of equine intestinal epithelial stem cells

2017 ◽  
Vol 50 (2) ◽  
pp. 241-248 ◽  
Author(s):  
A. Stieler Stewart ◽  
J. M. Freund ◽  
L. M. Gonzalez
Keyword(s):  
Stem Cells ◽  
Three Dimensional ◽  
Intestinal Epithelial ◽  
Epithelial Stem Cells ◽  
Three Dimensional Culture

scholarly journals Isolation of epithelial stem cells from dermis by a three-dimensional culture system

2006 ◽  
Vol 98 (1) ◽  
pp. 174-184 ◽  
Author(s):  
Reinhold J. Medina ◽  
Ken Kataoka ◽  
Mikiro Takaishi ◽  
Masahiro Miyazaki ◽  
Nam-ho Huh
Keyword(s):  
Stem Cells ◽  
Culture System ◽  
Three Dimensional ◽  
Epithelial Stem Cells ◽  
Three Dimensional Culture

Effects of Three-Dimensional Culture and Growth Factors on the Chondrogenic Differentiation of Murine Embryonic Stem Cells

Stem Cells ◽  
2006 ◽  
Vol 24 (2) ◽  
pp. 284-291 ◽  
Author(s):  
Nathaniel S. Hwang ◽  
Myoung Sook Kim ◽  
Somponnat Sampattavanich ◽  
Jin Hyen Baek ◽  
Zijun Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Embryonic Stem Cells ◽  
Chondrogenic Differentiation ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells ◽  
Three Dimensional Culture

Porcine spermatogonial stem cells self-renew effectively in a three dimensional culture microenvironment

2017 ◽  
Vol 41 (12) ◽  
pp. 1316-1324 ◽  
Author(s):  
Ji Eun Park ◽  
Min Hee Park ◽  
Min Seong Kim ◽  
Yeo Reum Park ◽  
Jung Im Yun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Three Dimensional ◽  
Spermatogonial Stem Cells ◽  
Three Dimensional Culture

scholarly journals Substrate-independent immunomodulatory characteristics of mesenchymal stem cells in three-dimensional culture

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0206811 ◽  
Author(s):  
Jing Li ◽  
Tong Chen ◽  
Xiahe Huang ◽  
Yunshan Zhao ◽  
Bin Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
Three Dimensional Culture ◽  
Substrate Independent

scholarly journals Unveiling role of Sphingosine-1-phosphate receptor 2 as a brake of epithelial stem cell proliferation and a tumor suppressor in colorectal cancer

2020 ◽  
Author(s):  
Luciana Petti ◽  
Giulia Rizzo ◽  
Federica Rubbino ◽  
Sudharshan Elangovan ◽  
Piergiuseppe Colombo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Normal Mucosa ◽  
Genetically Engineered ◽  
Colorectal Carcinogenesis ◽  
Intestinal Stem Cells ◽  
Cancer Information ◽  
Intestinal Epithelial ◽  
Epithelial Stem Cells ◽  
Mucosal Regeneration

Abstract BackgroundSphingosine-1-phosphate receptor 2 (S1PR2) mediates pleiotropic functions encompassing cell proliferation, survival, and migration, which become collectively de-regulated in cancer. Information onto whether S1PR2 participates in colorectal carcinogenesis/cancer is scanty, and we set out to fill the gap.MethodsWe screened expression changes of S1PR2 in human CRC and matched normal mucosa specimens [N = 76]. We compared CRC arising in inflammation-driven and genetically engineered models in wild-type (S1PR2+/+) and S1PR2 deficient (S1PR2−/−) mice. We reconstituted S1PR2 expression in RKO cells and assessed their growth in xenografts. Functionally, we mimicked ablation of S1PR2 in normal mucosa by treating S1PR2+/+ organoids with JTE013, and characterized intestinal epithelial stem cells isolated from S1PR2−/−Lgr5-EGFP- mice.ResultsS1PR2 expression was lost in 33% of CRC; in 55%, it was significantly decreased, only 12% retaining expression comparable to normal mucosa. Both colitis-induced and genetic Apc+/min mouse models of CRC showed a higher incidence in size and number of carcinomas and/or high-grade adenomas, with increased cell proliferation in S1PR2−/− mice compared to S1PR2+/+ controls. Loss of S1PR2 impaired mucosal regeneration, ultimately promoting the expansion of intestinal stem cells. Whereas its overexpression attenuated cell cycle progression, it reduced the phosphorylation of AKT and augmented the levels of PTEN.ConclusionsIn normal colonic crypts, S1PR2 gains expression along with intestinal epithelial cells differentiation, but not in intestinal stem cells, and contrasts intestinal tumorigenesis by promoting epithelial differentiation, preventing the expansion of stem cells and braking their malignant transformation. Targeting of S1PR2 may be of therapeutic benefit for CRC expressing high Lgr5.

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