Comparison of the colic incidence in a horse population with or without inclusion of germinated barley in the diet

2020 ◽  
Vol 32 (S11) ◽  
pp. 28-32 ◽  
Author(s):  
L. Troya ◽  
J. Blanco ◽  
I. Romero ◽  
M. Re
Keyword(s):  
Horse Population ◽  
Germinated Barley

Prebiotic treatment of experimental colitis with germinated barley foodstuff: A comparison with probiotic or antibiotic treatment

2001 ◽  
Vol 120 (5) ◽  
pp. A676-A676
Author(s):  
O KANAUCHI ◽  
K BREWERY ◽  
K MITSUYAMA ◽  
A ANDOH ◽  
Y ARAKI ◽  
...  
Keyword(s):  
Antibiotic Treatment ◽  
Experimental Colitis ◽  
Germinated Barley

scholarly journals Serological Survey of Leptospira Infection in Arabian Horses in Poland

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 688
Author(s):  
Bernard Wasiński ◽  
Katarzyna Paschalis-Trela ◽  
Jan Trela ◽  
Michał Czopowicz ◽  
Jerzy Kita ◽  
...  
Keyword(s):  
Companion Animals ◽  
Laboratory Diagnosis ◽  
Serological Survey ◽  
Serum Samples ◽  
Horse Population ◽  
Zoonotic Infections ◽  
Clinical Presentations ◽  
Arabian Horse ◽  
Breeding Farms

Leptospirosis is one of the most common zoonotic infections worldwide, including in most livestock, some companion animals, horses, wildlife, and humans. Epidemiological estimation of its prevalence in all species is difficult due to the variety of clinical presentations and challenges regarding laboratory diagnosis. The purpose of this study was to measure the seroprevalence of leptospiral infection in Arabian horses kept in the largest breeding farms in Poland, representing over 15% of the Polish Arabian horse population. Leptospira antibodies were detected by MAT (cut-off 1:100) in 33.2% of serum samples (204 of 615 animals) (CI 95%: 29.6–37.0%), most frequently reacting with the serovar Grippotyphosa, similar to previous reports in populations of randomly selected horses. These results indicated high Leptospira seropositivity, thus, although any form of clinical leptospirosis is rare, it may be postulated that the leptospiral exposure is widespread.

Unwanted Horse Population in Illinois: Perceptions of Horse Owners, Non-Horse Owners, and Equine Industry Stakeholders

2018 ◽  
Vol 22 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Kelsey Myers ◽  
Fatima Mohammed ◽  
Justin W. Rickard ◽  
Donald E. Meyer ◽  
Aslihan D. Spaulding
Keyword(s):  
Horse Population ◽  
Equine Industry ◽  
Horse Owners

scholarly journals GENETIC STRUCTURE OF HORSES POPULATION FOR THE GENES OF SCID AND HYPP

2017 ◽  
Vol 53 ◽  
pp. 248-253
Author(s):  
E. S. Cheburanova ◽  
O. A. Epishko ◽  
T. I. Kuzmina
Keyword(s):  
Genetic Structure ◽  
Severe Combined Immunodeficiency ◽  
Genetic Diagnosis ◽  
Hereditary Disease ◽  
Periodic Paralysis ◽  
Combined Immunodeficiency ◽  
Hyperkalemic Periodic Paralysis ◽  
Horse Population ◽  
Horse Farms ◽  
The Republic

Objective: identification of carrier animals of the hereditary anomaly SCID and HYPP of the population of horses bred on breeding horse farms of the Republic of Belarus. Methods: genetic, biological. DNA diagnosis of genotypes in the gene severe combined immunodeficiency (SCID) and hyperkaliemic periodic paralysis (HYPP) was performed using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) in the research laboratory «DNA Technology» of educational establishment «Grodno State Agrarian University». We tested 50 horses of different breeds, kept on different breeding horse farms of Belarus. DNA was isolated from buccal epithelium by perchlorate method with double purification (according to the method of Zinovieva). A study of the genetic structure of the horse population for hereditary anomalies of severe combined immunodeficiency and hyperkalemic periodic paralysis was performed using modern equipment. Amplification was carried out using the C1000 Touch thermal cycler (BioRad, USA). The visualization of the gel was carried out using the gel-documenting system Gel Doc XR + (Bio- Rad, USA). Main results. The use of modern methods for the genetic diagnosis of hereditary diseases of agricultural animals not only at birth to exclude from the process of reproduction of animal carriers of hereditary anomalies, but also reduces funding for their maintenance and cultivation. In the studies we used the PCR method based on the use of specific oligonucleotides with which the amplification of the necessary fragment of the 163 gene occurs in healthy individuals and 158 in the mutation carriers, which makes it possible to identify the hereditary SCID disease. We tested 50 animals of the upper and Arabian breeds, as well as horses with an admixture of Arabian blood, among which no carriers of the hereditary anomaly were found. In studies, the PCR-RFLP analysis method was used, based on the use of distinctive primers due to which the necessary fragment of the gene is amplified, which makes it possible to identify the hereditary HYPP disease. Amplified fragments were subjected to the action of restriction enzymes, with the help of which it is possible to determine the genotype of the animal under study. We tested 50 animals of the upper and Arabian breeds, as well as horses with an admixture of Arabian blood, among which no carriers of the hereditary anomaly were found. Conclusions. Severe combined immunodeficiency (SCID) is an autosomal recessive disease that occurs in humans, mice, horses and dogs. Carriers of this hereditary disease are born without visible anomalies, but after 2 weeks they become ill from any infection and die, since they can not develop an antigen-specific immune response. Hyperkaliemic periodic paralysis (HYPP) is an autosomal dominant disease that occurs in horses at the age of 2, when the animal begins, is strengthened to exercise. Symptoms of this disease are weakness of the muscles, periodic spasms, paralysis, which can lead to death. Presumably, there were no carriers of severe combined immunodeficiency in the Republic of Belarus, since breeding farms for breeding horses did not use biological material of Arabian and local breeds of horses for insemination. With subsequent importation of Arabian horses, as well as breeds that have an admixture of Arab blood, it is necessary to conduct DNA testing for the presence of severe combined immunodeficiency (SCID). According to the results of the conducted studies to identify the hereditary disease of hyperkalemic periodic paralysis (HYPP) in the horse population, no carriers were found, as well as sick animals. Presumably, there were no carriers of this disease on the territory of the republic, as evidenced by parallel studies conducted in other European countries, where no carrier or sick animal was found. This suggests that the disease was localized during the time, and also did not leave the territory of the American continent.

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