scholarly journals Addendum: EPPO Diagnostic Protocol PM 7/117 (1) Hymenoscyphus pseudoalbidus

EPPO Bulletin ◽  
2019 ◽  
Vol 50 (2) ◽  
pp. 358-358
Keyword(s):  
Diagnostic Protocol ◽  
Hymenoscyphus Pseudoalbidus

Space reconstruction of the aneurysms of the vertebrobasilary confluence based on conventional angiography: Our experience

2008 ◽  
Vol 55 (2) ◽  
pp. 75-78
Author(s):  
I.M. Nikolic ◽  
M.Lj. Rakic ◽  
E.E. Slavik ◽  
G.M. Tasic ◽  
B.M. Djurovic ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Diagnostic Procedures ◽  
Conventional Angiography ◽  
Two Dimensional Image ◽  
Diagnostic Protocol ◽  
Clinical Center ◽  
Golden Standard ◽  
Preoperative Diagnostic ◽  
Third Dimension ◽  
Initial Results

Besides current development of the new diagnostic procedures conventional angiography still represents the golden standard in the diagnosing of intracranial aneurysms. Since it gives a two-dimensional image if the presentation of the third dimension is wanted it is necessary to apply appropriate algorithm structures and computers. In this study we show our experience in the application of space reconstruction of blood vessels and aneurysms of the vertebrobasilary confluence in 6 patients operated at the Institute for Neurosurgery, Clinical Center of Serbia. Intraoperative finding in all patients matched the finding that we got by space reconstruction of the blood vessels, which was possible to observe from different angles. Postoperative course in all patients was satisfying. Upon discharge the patients were without rough lateralization of the pyramidal system. Our initial results and their practical agreement with the interoperative finding give us right to recommend this method as the standard for the preoperative diagnostic protocol.

scholarly journals AB0396 DIAGNOSTIC PROTOCOL INCLUDING MINIMALLY INVASIVE MINOR SALIVARY GLANDS BIOPSY IN SJÖGREN’S SYNDROME IN A SPANISH REFERENCE CENTER

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1497.1-1498
Author(s):  
J. Álvarez Troncoso ◽  
C. M. Oñoro López ◽  
C. Soto Abánades ◽  
E. Ruiz Bravo ◽  
L. Ramos Ruperto ◽  
...  
Keyword(s):  
Sjögren’S Syndrome ◽  
Sjögren's Syndrome ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Minor Salivary Glands ◽  
Schirmer Test ◽  
Schirmer’S Test ◽  
Diagnostic Protocol ◽  
Sjögren‘S Syndrome

Background:Sjögren’s syndrome (SjS) is a systemic autoimmune disease with a broad clinical presentation from dry syndrome to systemic extraglandular manifestations. The diagnosis can be complex since none of the markers, except anti-Ro, is sufficiently sensitive or specific. Although, minor salivary glands biopsy (MSGB), Schirmer’s test and unstimulated whole salivary flows (UWSF) are the hallmark for the diagnosis of this entity, its use is not widespread in some centers.Objectives:The aim of the study was to analyze the usefulness and safety of the diagnostic protocol for the classification of SjS and the immunological and analytical markers in dry syndrome due to SjS.Methods:Prospective observational study of a cohort of patients with sicca syndrome from a reference center. The diagnostic protocol (Schirmer’s test, UWSF and minimally invasive MSGB) was applied in the same consultation. Demographic, clinical, analytical and histological data were reviewed.Results:Over a period of 6 months, 48 patients with dry syndrome were analyzed, of which 39 women (81.2%). The main suspicion was SjS (39), followed by sarcoidosis (3), IgG4-related disease (2) and other diagnoses (4). The mean age was 59.1±4.4 years. Almost half (45.8%) reported xerostomia and 41.6% xerophthalmia. Recurrent parotidomegaly was described in 6 patients (12.5%) and arthralgias in 12 (25%). Immunologically, 23 (47.9%) presented anti-nuclear antibodies, 13 (27.1%) anti-Ro, 4 (8.3%) anti-La, 12 (25%) rheumatoid factor and 15 (31.2%) low C4. Schirmer test was positive in 32 patients (66.7%), UWSF in 22 (45.8%) and 9 (18.8%) had a focus score ≥1, although 16 (33.3%) had focal lymphocytic sialadenitis in the MSGB. A total of 21 (43,8%) patients were classified according to the 2016 ACR/EULAR criteria. 12 (57.1%) were seropositive SjS and 9 (42.9%) seronegative SjS. MSGB sensitivity was 71% and specificity 96%. Patient reported symptoms were unhelpful to differentiate SjS from other causes of dry syndrome. The number of protocols needed to diagnose a SjS was 2.28 (5.33 in seronegative SjS). Complications associated with the procedure were low (1 of 48) and mild (self-limited paraesthesia). Patients with SjS, unlike those with dry syndrome of another etiology, had more anemia (p<0.001), lymphopenia (p=0.022), ESR (p=0.030), beta-2 microglobulin (p=0.011), ANA (p<0.001), anti-ENA (p=0.006), anti-Ro (p<0.001), low C4 (p<0.001) and hypergammaglobulinemia (p=0.002).Conclusion:Immunological and histological manifestations were more predictive than clinical ones to differentiate SjS from other causes of dry syndrome. MSGB is a simple, sensitive, specific and safe procedure. The application of the diagnostic protocol (Schirmer test, UWSF and MSGB) allowed to standardize the classification of SjS and increased the diagnosis of patients with seronegative SjS.References:[1]Ramos-Casals M, Brito-Zerón P, Bombardieri S On behalf of the EULAR-Sjögren Syndrome Task Force Group, et al. EULAR recommendations for the management of Sjögren’s syndrome with topical and systemic therapies.Annals of the Rheumatic Diseases2020;79:3-18.[2]Guellec D, Cornec D, Jousse-Joulin S, et al. Diagnostic value of labial minor salivary gland biopsy for Sjögren’s syndrome: a systematic review.Autoimmun Rev. 2013;12(3):416–420.Disclosure of Interests:None declared

The diagnostic protocol in children and adolescents with syncope: a multi-centre prospective study

2009 ◽  
Vol 98 (5) ◽  
pp. 879-884 ◽  
Author(s):  
Qingyou Zhang ◽  
Junbao Du ◽  
Cheng Wang ◽  
Zhongdong Du ◽  
Li Wang ◽  
...  
Keyword(s):  
Prospective Study ◽  
Children And Adolescents ◽  
Diagnostic Protocol

Dryness Symptoms, Diagnostic Protocol and Therapeutic Management: A Report on 1,200 Patients

2001 ◽  
Vol 33 (4) ◽  
pp. 221-227 ◽  
Author(s):  
Piera Versura ◽  
Mauro Cellini ◽  
Alberto Torreggiani ◽  
Vincenzo Profazio ◽  
Benedetta Bernabini ◽  
...  
Keyword(s):  
Therapeutic Management ◽  
Diagnostic Protocol

Cryptosporidiosis: A Diagnostic Protocol

1985 ◽  
Vol 16 (2) ◽  
pp. 105-106 ◽  
Author(s):  
Diana Garza ◽  
Bernard Marino ◽  
Mary Wilson
Keyword(s):  
Diagnostic Protocol

scholarly journals Sensitive and Rapid Detection of Edwardsiellosis in Fish by a Loop-Mediated Isothermal Amplification Method

2004 ◽  
Vol 70 (1) ◽  
pp. 621-624 ◽  
Author(s):  
Ram Savan ◽  
Arisa Igarashi ◽  
Satoru Matsuoka ◽  
Masahiro Sakai
Keyword(s):  
Rapid Detection ◽  
Japanese Flounder ◽  
Isothermal Amplification ◽  
Edwardsiella Tarda ◽  
Fish Disease ◽  
Fish Pathogen ◽  
Loop Mediated Isothermal Amplification ◽  
Diagnostic Protocol ◽  
Amplification Method ◽  
Hemolysin Gene

ABSTRACT Here we report a rapid and sensitive method (using loop-mediated isothermal amplification [LAMP]) for the diagnosis of edwardsiellosis, a fish disease caused by Edwardsiella tarda, in Japanese flounder. A set of four primers was designed, and conditions for the detection were optimized for the detection of E. tarda in 45 min at 65°C. No amplification of the target hemolysin gene was detected in other related bacteria. When the LAMP primers were used, detection of edwardsiellosis in infected Japanese flounder kidney, and spleen and seawater cultures was possible. We have developed a rapid and sensitive diagnostic protocol for edwardsiellosis detection in fish. This is the first report of the application of LAMP for the diagnosis of a fish pathogen.

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