Survival of Ralstonia solanacearum and Ralstonia pseudosolanacearum in drain water

EPPO Bulletin ◽  
2018 ◽  
Vol 48 (1) ◽  
pp. 97-104
Author(s):  
L. H. Stevens ◽  
P. S. van der Zouwen ◽  
C. A. M. van Tongeren ◽  
P. Kastelein ◽  
J. M. van der Wolf
Keyword(s):  
Ralstonia Solanacearum ◽  
Drain Water ◽  
Ralstonia Pseudosolanacearum

Genomic and Biological Characterization of Ralstonia solanacearum Inovirus Brazil 1, an Inovirus that Alters the Pathogenicity of the Phytopathogen Ralstonia pseudosolanacearum

2021 ◽  
Author(s):  
Juliana Cristina Fraleon de Almeida ◽  
André da Silva Xavier ◽  
Renan de Souza Cascardo ◽  
Rafael Reis de Rezende ◽  
Flavia Oliveira de Souza ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Biological Characterization ◽  
Ralstonia Pseudosolanacearum

scholarly journals First Report of Bacterial Wilt of Tomato Caused by Ralstonia pseudosolanacearum (Ralstonia solanacearum phylotype I) in Cambodia

Plant Disease ◽  
2020 ◽  
Vol 104 (3) ◽  
pp. 969-969
Author(s):  
T. L. Klass ◽  
M. M. Hayes ◽  
K. H. Seng ◽  
C. An ◽  
F. Rotondo ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
First Report ◽  
Ralstonia Pseudosolanacearum ◽  
Phylotype I

scholarly journals Detection of Ralstonia pseudosolanacearum in drain water based on concentration, enrichment and the use of a duplex TaqMan PCR test

EPPO Bulletin ◽  
2020 ◽  
Vol 50 (2) ◽  
pp. 340-349
Author(s):  
N. Sedighian ◽  
O. Mendes ◽  
L. Poleij ◽  
P. Bonants ◽  
J. Wolf
Keyword(s):  
Drain Water ◽  
Water Based ◽  
Ralstonia Pseudosolanacearum ◽  
Taqman Pcr ◽  
Pcr Test

scholarly journals Phylogenetic Assignment of Ralstonia pseudosolanacearum (Ralstonia solanacearum Phylotype I) Isolated from Rosa spp.

Plant Disease ◽  
2018 ◽  
Vol 102 (11) ◽  
pp. 2258-2267 ◽  
Author(s):  
M. Bergsma-Vlami ◽  
J. L. J. van de Bilt ◽  
N. N. A. Tjou-Tam-Sin ◽  
M. Westenberg ◽  
E. T. M. Meekes ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Phylogenetic Trees ◽  
Solanum Melongena ◽  
Monophyletic Group ◽  
Phylogenetic Position ◽  
Bacterial Disease ◽  
Cut Flowers ◽  
Ralstonia Pseudosolanacearum ◽  
The One ◽  
Phylotype I

During the last two years, greenhouse cultivation of rose (Rosa spp.) in the Netherlands has been challenged by an uncommon bacterial disease. Affected plants suffered from chlorosis, stunting, wilting, and necrosis. The bacterial isolates obtained from the different Rosa spp. cultivars were all identified as phylotype I, sequevar 33 from the ‘Ralstonia solanacearum species complex’ (RSSC), actually reclassified as Ralstonia pseudosolanacearum. The work in this paper considers the genetic diversity and the phylogenetic position of 129 R. pseudosolanacearum isolates from the outbreak. This was assessed by AFLP based on four different primer combinations and MLP using partial sequences of the egl, mutS, and fliC genes. The AFLP revealed identical profiles for all the isolates, irrespective of their association with Rosa sp. propagating material, Rosa spp. plants for cut flowers, or water used in the different greenhouse cultivations. These AFLP profiles were unique and diverged from profiles of all other reference isolates in the RSSC included. Furthermore, MLP on egl, fliC, and mutS gene sequences clearly demonstrated that all R. pseudosolanacearum isolates clustered in phylotype I, as a distinct monophyletic group. Interestingly, this monophyletic group also included phylotype I strain Rs-09-161 from eggplant (Solanum melongena), isolated in 2009 in India. AFLP and MLP were both efficient in revealing the genetic divergence from the RSSC isolates included. The phylogenetic tree constructed from the AFLP profiles was, in general, in agreement with the one obtained from MLP. Both phylogenetic trees displayed a similar clustering, supported by high posterior probabilities. Both methodologies clearly demonstrated that the R. pseudosolanacearum isolates from Rosa spp. grouped in a monophyletic group inside phylotype I, with a particular correspondence to a strain present in India, as revealed in MLP.

scholarly journals Low Temperature Induces Infectious Nonresuscitatable VBNC Cells in Ralstonia Pseudosolanacearum

2021 ◽  
Author(s):  
Neha Faridi ◽  
Merwyn Packia raj Samuel ◽  
Shalini Bhatt ◽  
Ankur Agrawal ◽  
Veena Pande ◽  
...  
Keyword(s):  
Low Temperature ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Species Complex ◽  
Economic Importance ◽  
Bacterial Genus ◽  
Window Period ◽  
Ralstonia Pseudosolanacearum ◽  
Uv C

Abstract Ralstonia pseudosolanacearum and other members of Ralstonia solanacearum species complex (RSSC) causes the disease bacterial wilt in many crops of economic importance. The organism is known to form Viable But Non Culturable cells (VBNC). VBNCs resuscitate invitro during the “resuscitation window” period and are infectious Previous studies have identified nonresuscitatable VBNCs in various bacterial genus including RSSC, however their infectivity was not elucidated and described. In this work, VBNCs of two Ralstonia pseudosolanacearum strains were generated by exposing the microcosms to psychrophilic stress, UV-C radiation and 70% isopropanol. Both resuscitatable and nonresuscitatable VBNCs were observed in psychrophilic and UV-C stressed microcosms. The nonresuscitatable VBNCs generated at psychrophilic temperature were found infective. Based on resuscitation properties, nonresuscitatable VBNCs can be considered as a different VBNC type from resuscitatable VBNCs.

scholarly journals Identificação de famílias F2:3 de tomateiro homozigotas resistentes à Ralstonia pseudosolanacearum e Ralstonia solanacearum

2018 ◽  
Vol 13 (3) ◽  
pp. 270
Author(s):  
Kleyton Danilo da Silva Costa ◽  
Ana Maria Maciel dos Santos ◽  
Paulo Ricardo dos Santos ◽  
Adriano Márcio Freire Silva ◽  
Eduardo Pereira de Sousa Neto ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Ralstonia Pseudosolanacearum

Objetivou-se identificar famílias F2:3 de tomateiro homozigotas resistentes a Ralstonia pseudosolanacearum e Ralstonia solanacearum. Foram conduzidos dois experimentos em casa de vegetação da Universidade Federal Rural de Pernambuco sendo um para cada patógeno. Foram avaliados 45 tratamentos compostos pelas cultivares parentais Yoshimatsu (resistente) e IPA-7 (susceptível) e 43 famílias F2:3 obtidas a partir da geração F2. Cada tratamento foi constituído por 16 plantas. Foram avaliadas a incidência de murcha bacteriana por meio de uma escala descritiva de notas aos 20 dias após a inoculação. As famílias foram comparadas com os parentais de acordo com a frequência de plantas obtidas para cada nota. Foi realizado o teste qui quadrado (χ_c^2) obtendo-se a significância em relação a cada um dos genitores. Para a espécie R. pseudosolanacearum foram identificadas três famílias homozigotas resistentes: Família F2:3#04, Família F2:3#29 e Família F2:3#31. Com relação a espécie R. solanacearum foram identificadas duas famílias homozigotas resistentes: Família F2:3#41 e Família F2:3#42. Estas famílias indicam a possibilidade de novas linhagens resistentes que poderão ser utilizadas em programas de melhoramento.

scholarly journals Polyphasic taxonomic revision of the Ralstonia solanacearum species complex: proposal to emend the descriptions of Ralstonia solanacearum and Ralstonia syzygii and reclassify current R. syzygii strains as Ralstonia syzygii subsp. syzygii subsp. nov., R. solanacearum phylotype IV strains as Ralstonia syzygii subsp. indonesiensis subsp. nov., banana blood disease bacterium strains as Ralstonia syzygii subsp. celebesensis subsp. nov. and R. solanacearum phylotype I and III strains as Ralstonia pseudosolanacearum sp. nov.

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3087-3103 ◽  
Author(s):  
Irda Safni ◽  
Ilse Cleenwerck ◽  
Paul De Vos ◽  
Mark Fegan ◽  
Lindsay Sly ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Type Strain ◽  
Type Species ◽  
Species Complex ◽  
23S Rrna ◽  
Gene Sequences ◽  
Content Type ◽  
Blood Disease ◽  
Link Type ◽  
Ralstonia Pseudosolanacearum

The Ralstonia solanacearum species complex has long been recognized as a group of phenotypically diverse strains that can be subdivided into four phylotypes. Using a polyphasic taxonomic approach on an extensive set of strains, this study provides evidence for a taxonomic and nomenclatural revision of members of this complex. Data obtained from phylogenetic analysis of 16S-23S rRNA ITS gene sequences, 16S–23S rRNA intergenic spacer (ITS) region sequences and partial endoglucanase (egl) gene sequences and DNA–DNA hybridizations demonstrate that the R. solanacearum species complex comprises three genospecies. One of these includes the type strain of Ralstonia solanacearum and consists of strains of R. solanacearum phylotype II only. The second genospecies includes the type strain of Ralstonia syzygii and contains only phylotype IV strains. This genospecies is subdivided into three distinct groups, namely R. syzygii , the causal agent of Sumatra disease on clove trees in Indonesia, R. solanacearum phylotype IV strains isolated from different host plants mostly from Indonesia, and strains of the blood disease bacterium (BDB), the causal agent of the banana blood disease, a bacterial wilt disease in Indonesia that affects bananas and plantains. The last genospecies is composed of R. solanacearum strains that belong to phylotypes I and III. As these genospecies are also supported by phenotypic data that allow the differentiation of the three genospecies, the following taxonomic proposals are made: emendation of the descriptions of Ralstonia solanacearum and Ralstonia syzygii and descriptions of Ralstonia syzygii subsp. nov. (type strain R 001T = LMG 10661T = DSM 7385T) for the current R. syzygii strains, Ralstonia syzygii subsp. indonesiensis subsp. nov. (type strain UQRS 464T = LMG 27703T = DSM 27478T) for the current R. solanacearum phylotype IV strains, Ralstonia syzygii subsp. celebesensis subsp. nov. (type strain UQRS 627T = LMG 27706T = DSM 27477T) for the BDB strains and Ralstonia pseudosolanacearum sp. nov. (type strain UQRS 461T = LMG 9673T = NCPPB 1029T) for the strains of R. solanacearum phylotypes I and III.

scholarly journals Genetic Structure of Ralstonia solanacearum and Ralstonia pseudosolanacearum in Brazil

Plant Disease ◽  
2020 ◽  
Vol 104 (4) ◽  
pp. 1019-1025 ◽  
Author(s):  
Thaís Ribeiro Santiago ◽  
Carlos Alberto Lopes ◽  
Gustavo Caetano-Anollés ◽  
Eduardo S. G. Mizubuti
Keyword(s):  
Genetic Variability ◽  
Ralstonia Solanacearum ◽  
The Other ◽  
Potato Tubers ◽  
Migration Rates ◽  
The North ◽  
Native Soil ◽  
The World ◽  
Ralstonia Pseudosolanacearum ◽  
Geographic Regions

Bacterial wilt-causing Ralstonia threaten numerous crops throughout the world. We studied the population structure of 196 isolates of Ralstonia solanacearum and 39 isolates of Ralstonia pseudosolanacearum, which were collected from potato- and tomato-growing areas in 19 states of Brazil. Regardless of the species, three groups of isolates were identified. One group encompassed R. pseudosolanacearum isolates. The other two groups comprise isolates of R. solanacearum (phylotype II) split according to geographic regions, one made of isolates from the North and Northeast and the other made of isolates from the Central, Southeast, and South regions (CSS). Among the isolates collected in CSS, those from tomato were genetically distinct from the potato isolates. The genetic variability in the population of R. pseudosolanacearum was lower than that of R. solanacearum, suggesting that the former was introduced in Brazil. Conversely, the high genetic variability of R. solanacearum in all regions, hosts, and times supports the hypothesis that this species is autochthonous in South America, more precisely in Brazil and Peru. For R. solanacearum, higher variability and lower migration rates were observed when tomato isolates were analyzed, indicating that the variability is caused mainly by the differences of the local, native soil population. The North subpopulation was distinct from all others, possibly because of differences in environmental features of this region. The proximity of some geographic regions and the movement of potato tubers could have facilitated migration and therefore low genetic differentiation between geographic regions. Finally, geography, which also influences host distribution, affects the structure of the population of R. solanacearum in Brazil. Despite quarantine procedures in Brazil, increasing levels of trade are a threat to biosecurity, and these results emphasize the need for improving our regional efforts to prevent the dispersal of pathogens.

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